Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03945

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03940 and BWI76_RS03945 are separated by 11 nucleotidesBWI76_RS03945 and BWI76_RS03950 are separated by 136 nucleotides BWI76_RS03940: BWI76_RS03940 - alcohol dehydrogenase, at 814,109 to 815,131 _RS03940 BWI76_RS03945: BWI76_RS03945 - altronate oxidoreductase, at 815,143 to 816,588 _RS03945 BWI76_RS03950: BWI76_RS03950 - sigma-54 interacting domain-containing protein, at 816,725 to 819,490 _RS03950 Position (kb) 815 816 817Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3 4at 814.243 kb on + strand, within BWI76_RS03940at 814.245 kb on + strand, within BWI76_RS03940at 814.265 kb on + strand, within BWI76_RS03940at 814.308 kb on + strand, within BWI76_RS03940at 814.356 kb on + strand, within BWI76_RS03940at 814.361 kb on + strand, within BWI76_RS03940at 814.369 kb on - strandat 814.391 kb on + strand, within BWI76_RS03940at 814.391 kb on + strand, within BWI76_RS03940at 814.391 kb on + strand, within BWI76_RS03940at 814.392 kb on - strand, within BWI76_RS03940at 814.398 kb on - strand, within BWI76_RS03940at 814.399 kb on - strand, within BWI76_RS03940at 814.399 kb on - strand, within BWI76_RS03940at 814.399 kb on - strand, within BWI76_RS03940at 814.399 kb on - strand, within BWI76_RS03940at 814.399 kb on - strand, within BWI76_RS03940at 814.399 kb on - strand, within BWI76_RS03940at 814.401 kb on + strand, within BWI76_RS03940at 814.409 kb on - strand, within BWI76_RS03940at 814.472 kb on - strand, within BWI76_RS03940at 814.540 kb on + strand, within BWI76_RS03940at 814.549 kb on - strand, within BWI76_RS03940at 814.630 kb on - strand, within BWI76_RS03940at 814.640 kb on - strand, within BWI76_RS03940at 814.692 kb on + strand, within BWI76_RS03940at 814.700 kb on - strand, within BWI76_RS03940at 814.702 kb on + strand, within BWI76_RS03940at 814.737 kb on + strand, within BWI76_RS03940at 814.745 kb on - strand, within BWI76_RS03940at 814.781 kb on + strand, within BWI76_RS03940at 814.835 kb on + strand, within BWI76_RS03940at 814.894 kb on + strand, within BWI76_RS03940at 814.902 kb on - strand, within BWI76_RS03940at 814.902 kb on - strand, within BWI76_RS03940at 814.902 kb on - strand, within BWI76_RS03940at 815.012 kb on + strand, within BWI76_RS03940at 815.028 kb on + strand, within BWI76_RS03940at 815.205 kb on - strandat 815.214 kb on - strandat 815.332 kb on + strand, within BWI76_RS03945at 815.456 kb on + strand, within BWI76_RS03945at 815.464 kb on - strand, within BWI76_RS03945at 815.692 kb on + strand, within BWI76_RS03945at 815.728 kb on + strand, within BWI76_RS03945at 815.751 kb on - strand, within BWI76_RS03945at 815.835 kb on + strand, within BWI76_RS03945at 816.026 kb on - strand, within BWI76_RS03945at 816.298 kb on - strand, within BWI76_RS03945at 816.414 kb on - strand, within BWI76_RS03945at 816.427 kb on + strand, within BWI76_RS03945at 816.444 kb on + strandat 816.910 kb on + strandat 816.976 kb on - strandat 817.081 kb on + strand, within BWI76_RS03950at 817.081 kb on + strand, within BWI76_RS03950at 817.081 kb on + strand, within BWI76_RS03950at 817.104 kb on - strand, within BWI76_RS03950at 817.104 kb on - strand, within BWI76_RS03950at 817.262 kb on - strand, within BWI76_RS03950at 817.274 kb on + strand, within BWI76_RS03950at 817.389 kb on + strand, within BWI76_RS03950at 817.492 kb on - strand, within BWI76_RS03950

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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814,243 + BWI76_RS03940 0.13 -0.2
814,245 + BWI76_RS03940 0.13 +1.8
814,265 + BWI76_RS03940 0.15 +0.6
814,308 + BWI76_RS03940 0.19 +1.0
814,356 + BWI76_RS03940 0.24 -0.8
814,361 + BWI76_RS03940 0.25 +0.8
814,369 - +1.8
814,391 + BWI76_RS03940 0.28 -4.6
814,391 + BWI76_RS03940 0.28 -0.2
814,391 + BWI76_RS03940 0.28 +0.2
814,392 - BWI76_RS03940 0.28 -1.6
814,398 - BWI76_RS03940 0.28 +1.0
814,399 - BWI76_RS03940 0.28 -0.8
814,399 - BWI76_RS03940 0.28 -0.5
814,399 - BWI76_RS03940 0.28 -2.7
814,399 - BWI76_RS03940 0.28 +0.7
814,399 - BWI76_RS03940 0.28 -3.5
814,399 - BWI76_RS03940 0.28 +0.2
814,401 + BWI76_RS03940 0.29 -1.4
814,409 - BWI76_RS03940 0.29 -1.2
814,472 - BWI76_RS03940 0.35 -0.4
814,540 + BWI76_RS03940 0.42 -1.2
814,549 - BWI76_RS03940 0.43 -1.7
814,630 - BWI76_RS03940 0.51 +0.1
814,640 - BWI76_RS03940 0.52 -0.1
814,692 + BWI76_RS03940 0.57 +0.0
814,700 - BWI76_RS03940 0.58 +0.3
814,702 + BWI76_RS03940 0.58 -3.3
814,737 + BWI76_RS03940 0.61 -0.7
814,745 - BWI76_RS03940 0.62 +1.2
814,781 + BWI76_RS03940 0.66 -0.4
814,835 + BWI76_RS03940 0.71 -0.2
814,894 + BWI76_RS03940 0.77 +1.4
814,902 - BWI76_RS03940 0.78 -0.5
814,902 - BWI76_RS03940 0.78 -0.6
814,902 - BWI76_RS03940 0.78 -1.8
815,012 + BWI76_RS03940 0.88 -0.1
815,028 + BWI76_RS03940 0.90 +4.2
815,205 - -1.7
815,214 - -0.8
815,332 + BWI76_RS03945 0.13 -3.3
815,456 + BWI76_RS03945 0.22 +2.8
815,464 - BWI76_RS03945 0.22 -0.0
815,692 + BWI76_RS03945 0.38 +1.6
815,728 + BWI76_RS03945 0.40 -2.2
815,751 - BWI76_RS03945 0.42 +0.9
815,835 + BWI76_RS03945 0.48 +0.2
816,026 - BWI76_RS03945 0.61 -0.1
816,298 - BWI76_RS03945 0.80 +0.8
816,414 - BWI76_RS03945 0.88 +1.0
816,427 + BWI76_RS03945 0.89 -1.6
816,444 + +0.1
816,910 + -1.2
816,976 - +0.3
817,081 + BWI76_RS03950 0.13 +4.6
817,081 + BWI76_RS03950 0.13 +0.7
817,081 + BWI76_RS03950 0.13 +0.9
817,104 - BWI76_RS03950 0.14 +1.9
817,104 - BWI76_RS03950 0.14 -1.7
817,262 - BWI76_RS03950 0.19 -2.4
817,274 + BWI76_RS03950 0.20 +0.6
817,389 + BWI76_RS03950 0.24 +0.6
817,492 - BWI76_RS03950 0.28 -0.5

Or see this region's nucleotide sequence