Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS03145

Experiment: KLEB17 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS03140 and BWI76_RS03145 are separated by 233 nucleotidesBWI76_RS03145 and BWI76_RS03150 are separated by 164 nucleotides BWI76_RS03140: BWI76_RS03140 - alcohol dehydrogenase, at 647,173 to 648,192 _RS03140 BWI76_RS03145: BWI76_RS03145 - tRNA-Leu, at 648,426 to 648,510 _RS03145 BWI76_RS03150: BWI76_RS03150 - site-specific recombinase, phage integrase family protein, at 648,675 to 649,937 _RS03150 Position (kb) 648 649Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2 3at 647.610 kb on + strand, within BWI76_RS03140at 647.632 kb on - strand, within BWI76_RS03140at 647.891 kb on + strand, within BWI76_RS03140at 647.899 kb on - strand, within BWI76_RS03140at 647.945 kb on + strand, within BWI76_RS03140at 647.954 kb on + strand, within BWI76_RS03140at 647.954 kb on + strand, within BWI76_RS03140at 647.992 kb on + strand, within BWI76_RS03140at 647.992 kb on + strand, within BWI76_RS03140at 648.090 kb on - strand, within BWI76_RS03140at 648.195 kb on - strandat 648.217 kb on + strandat 648.237 kb on - strandat 648.241 kb on + strandat 648.285 kb on - strandat 648.288 kb on - strandat 648.309 kb on - strandat 648.685 kb on + strandat 648.691 kb on + strandat 648.695 kb on + strandat 648.703 kb on - strandat 648.710 kb on + strandat 648.841 kb on + strand, within BWI76_RS03150at 648.867 kb on - strand, within BWI76_RS03150at 648.869 kb on + strand, within BWI76_RS03150at 648.877 kb on - strand, within BWI76_RS03150at 648.877 kb on - strand, within BWI76_RS03150at 648.906 kb on + strand, within BWI76_RS03150at 648.906 kb on + strand, within BWI76_RS03150at 648.913 kb on + strand, within BWI76_RS03150at 648.921 kb on - strand, within BWI76_RS03150at 648.963 kb on + strand, within BWI76_RS03150at 649.056 kb on - strand, within BWI76_RS03150at 649.097 kb on + strand, within BWI76_RS03150at 649.097 kb on + strand, within BWI76_RS03150at 649.097 kb on + strand, within BWI76_RS03150at 649.112 kb on + strand, within BWI76_RS03150at 649.151 kb on + strand, within BWI76_RS03150at 649.159 kb on - strand, within BWI76_RS03150at 649.206 kb on + strand, within BWI76_RS03150at 649.214 kb on - strand, within BWI76_RS03150at 649.225 kb on + strand, within BWI76_RS03150at 649.225 kb on + strand, within BWI76_RS03150at 649.233 kb on - strand, within BWI76_RS03150at 649.233 kb on - strand, within BWI76_RS03150at 649.248 kb on - strand, within BWI76_RS03150at 649.277 kb on + strand, within BWI76_RS03150at 649.287 kb on + strand, within BWI76_RS03150at 649.295 kb on - strand, within BWI76_RS03150at 649.329 kb on + strand, within BWI76_RS03150at 649.363 kb on + strand, within BWI76_RS03150at 649.371 kb on - strand, within BWI76_RS03150at 649.489 kb on - strand, within BWI76_RS03150at 649.500 kb on - strand, within BWI76_RS03150at 649.500 kb on - strand, within BWI76_RS03150

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB17 MOI 1
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647,610 + BWI76_RS03140 0.43 +0.3
647,632 - BWI76_RS03140 0.45 +0.7
647,891 + BWI76_RS03140 0.70 +2.3
647,899 - BWI76_RS03140 0.71 -3.9
647,945 + BWI76_RS03140 0.76 +1.6
647,954 + BWI76_RS03140 0.77 -1.1
647,954 + BWI76_RS03140 0.77 +1.7
647,992 + BWI76_RS03140 0.80 -0.3
647,992 + BWI76_RS03140 0.80 -0.4
648,090 - BWI76_RS03140 0.90 +0.3
648,195 - +1.0
648,217 + -3.3
648,237 - +0.6
648,241 + -2.6
648,285 - -3.2
648,288 - +0.4
648,309 - +1.5
648,685 + -0.9
648,691 + +0.8
648,695 + +0.7
648,703 - +1.0
648,710 + +1.2
648,841 + BWI76_RS03150 0.13 -2.4
648,867 - BWI76_RS03150 0.15 +0.1
648,869 + BWI76_RS03150 0.15 -3.8
648,877 - BWI76_RS03150 0.16 -0.0
648,877 - BWI76_RS03150 0.16 +1.5
648,906 + BWI76_RS03150 0.18 -1.1
648,906 + BWI76_RS03150 0.18 +3.3
648,913 + BWI76_RS03150 0.19 -0.9
648,921 - BWI76_RS03150 0.19 +0.2
648,963 + BWI76_RS03150 0.23 +0.6
649,056 - BWI76_RS03150 0.30 -1.9
649,097 + BWI76_RS03150 0.33 +0.9
649,097 + BWI76_RS03150 0.33 +0.2
649,097 + BWI76_RS03150 0.33 -1.9
649,112 + BWI76_RS03150 0.35 -0.1
649,151 + BWI76_RS03150 0.38 -0.5
649,159 - BWI76_RS03150 0.38 -0.8
649,206 + BWI76_RS03150 0.42 -1.9
649,214 - BWI76_RS03150 0.43 +0.4
649,225 + BWI76_RS03150 0.44 -0.2
649,225 + BWI76_RS03150 0.44 +0.2
649,233 - BWI76_RS03150 0.44 +1.2
649,233 - BWI76_RS03150 0.44 +1.3
649,248 - BWI76_RS03150 0.45 +1.3
649,277 + BWI76_RS03150 0.48 +2.4
649,287 + BWI76_RS03150 0.48 +1.1
649,295 - BWI76_RS03150 0.49 +0.3
649,329 + BWI76_RS03150 0.52 -0.7
649,363 + BWI76_RS03150 0.54 -0.6
649,371 - BWI76_RS03150 0.55 +0.3
649,489 - BWI76_RS03150 0.64 -1.8
649,500 - BWI76_RS03150 0.65 +1.1
649,500 - BWI76_RS03150 0.65 +0.7

Or see this region's nucleotide sequence