Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02315 and BWI76_RS02320 overlap by 25 nucleotides BWI76_RS02320 and BWI76_RS02325 are separated by 94 nucleotides
BWI76_RS02315: BWI76_RS02315 - protein-disulfide reductase DsbD, at 475,843 to 477,600
_RS02315
BWI76_RS02320: BWI76_RS02320 - divalent ion tolerance protein CutA, at 477,576 to 477,899
_RS02320
BWI76_RS02325: BWI76_RS02325 - anaerobic C4-dicarboxylate transporter, at 477,994 to 479,295
_RS02325
Position (kb) 477
478 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 476.586 kb on + strand, within BWI76_RS02315 at 476.686 kb on + strand, within BWI76_RS02315 at 476.694 kb on - strand, within BWI76_RS02315 at 476.786 kb on - strand, within BWI76_RS02315 at 476.878 kb on + strand, within BWI76_RS02315 at 477.006 kb on - strand, within BWI76_RS02315 at 477.252 kb on + strand, within BWI76_RS02315 at 477.353 kb on - strand, within BWI76_RS02315 at 477.423 kb on + strand, within BWI76_RS02315 at 477.453 kb on + strand at 477.461 kb on - strand at 477.573 kb on - strand at 477.681 kb on + strand, within BWI76_RS02320 at 477.793 kb on - strand, within BWI76_RS02320 at 477.837 kb on + strand, within BWI76_RS02320 at 477.844 kb on - strand, within BWI76_RS02320 at 477.860 kb on + strand, within BWI76_RS02320 at 477.868 kb on - strand at 477.881 kb on - strand at 477.929 kb on + strand at 477.972 kb on - strand at 478.046 kb on - strand at 478.060 kb on + strand at 478.098 kb on + strand at 478.201 kb on - strand, within BWI76_RS02325 at 478.211 kb on - strand, within BWI76_RS02325 at 478.336 kb on + strand, within BWI76_RS02325 at 478.346 kb on + strand, within BWI76_RS02325 at 478.472 kb on + strand, within BWI76_RS02325 at 478.480 kb on - strand, within BWI76_RS02325 at 478.538 kb on - strand, within BWI76_RS02325 at 478.543 kb on + strand, within BWI76_RS02325 at 478.548 kb on + strand, within BWI76_RS02325 at 478.556 kb on - strand, within BWI76_RS02325 at 478.617 kb on - strand, within BWI76_RS02325 at 478.671 kb on - strand, within BWI76_RS02325 at 478.677 kb on - strand, within BWI76_RS02325 at 478.794 kb on + strand, within BWI76_RS02325
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 476,586 + BWI76_RS02315 0.42 +0.2 476,686 + BWI76_RS02315 0.48 -0.4 476,694 - BWI76_RS02315 0.48 -2.6 476,786 - BWI76_RS02315 0.54 -0.5 476,878 + BWI76_RS02315 0.59 +0.4 477,006 - BWI76_RS02315 0.66 +1.0 477,252 + BWI76_RS02315 0.80 +0.7 477,353 - BWI76_RS02315 0.86 +0.1 477,423 + BWI76_RS02315 0.90 +1.3 477,453 + -0.9 477,461 - +0.1 477,573 - -0.6 477,681 + BWI76_RS02320 0.32 -2.3 477,793 - BWI76_RS02320 0.67 -2.3 477,837 + BWI76_RS02320 0.81 -1.9 477,844 - BWI76_RS02320 0.83 -1.4 477,860 + BWI76_RS02320 0.88 -1.9 477,868 - -0.1 477,881 - -0.2 477,929 + +0.4 477,972 - -0.3 478,046 - +0.9 478,060 + -1.3 478,098 + -0.2 478,201 - BWI76_RS02325 0.16 +0.3 478,211 - BWI76_RS02325 0.17 -0.8 478,336 + BWI76_RS02325 0.26 -2.6 478,346 + BWI76_RS02325 0.27 +1.5 478,472 + BWI76_RS02325 0.37 +1.6 478,480 - BWI76_RS02325 0.37 +0.2 478,538 - BWI76_RS02325 0.42 -0.1 478,543 + BWI76_RS02325 0.42 -0.9 478,548 + BWI76_RS02325 0.43 -2.4 478,556 - BWI76_RS02325 0.43 +0.7 478,617 - BWI76_RS02325 0.48 +1.4 478,671 - BWI76_RS02325 0.52 -2.7 478,677 - BWI76_RS02325 0.52 -1.0 478,794 + BWI76_RS02325 0.61 -3.8
Or see this region's nucleotide sequence