Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01925 and BWI76_RS01930 are separated by 197 nucleotides BWI76_RS01930 and BWI76_RS01935 are separated by 141 nucleotides BWI76_RS01935 and BWI76_RS01940 are separated by 2 nucleotides
BWI76_RS01925: BWI76_RS01925 - aromatic amino acid aminotransferase, at 392,296 to 393,489
_RS01925
BWI76_RS01930: BWI76_RS01930 - acid phosphatase/phosphotransferase, at 393,687 to 394,400
_RS01930
BWI76_RS01935: BWI76_RS01935 - thiamin phosphate synthase, at 394,542 to 394,958
_RS01935
BWI76_RS01940: BWI76_RS01940 - hypothetical protein, at 394,961 to 395,314
_RS01940
Position (kb) 393
394
395 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1 at 392.840 kb on + strand, within BWI76_RS01925 at 392.860 kb on + strand, within BWI76_RS01925 at 392.988 kb on - strand, within BWI76_RS01925 at 393.187 kb on + strand, within BWI76_RS01925 at 393.212 kb on - strand, within BWI76_RS01925 at 393.263 kb on + strand, within BWI76_RS01925 at 393.273 kb on + strand, within BWI76_RS01925 at 393.280 kb on - strand, within BWI76_RS01925 at 393.281 kb on + strand, within BWI76_RS01925 at 393.369 kb on + strand, within BWI76_RS01925 at 393.371 kb on - strand at 393.371 kb on - strand at 393.371 kb on - strand at 393.390 kb on + strand at 393.457 kb on - strand at 393.457 kb on - strand at 393.463 kb on + strand at 393.484 kb on + strand at 393.493 kb on - strand at 393.504 kb on + strand at 393.512 kb on - strand at 393.531 kb on + strand at 393.531 kb on + strand at 393.531 kb on + strand at 393.539 kb on - strand at 393.583 kb on - strand at 393.699 kb on + strand at 393.706 kb on + strand at 393.823 kb on - strand, within BWI76_RS01930 at 393.823 kb on - strand, within BWI76_RS01930 at 393.838 kb on - strand, within BWI76_RS01930 at 394.227 kb on + strand, within BWI76_RS01930 at 394.285 kb on + strand, within BWI76_RS01930 at 394.369 kb on + strand at 394.377 kb on - strand at 394.473 kb on + strand at 394.488 kb on - strand at 394.560 kb on - strand at 394.637 kb on + strand, within BWI76_RS01935 at 394.858 kb on - strand, within BWI76_RS01935 at 394.862 kb on - strand, within BWI76_RS01935 at 394.934 kb on - strand at 394.994 kb on + strand at 395.004 kb on + strand, within BWI76_RS01940 at 395.009 kb on + strand, within BWI76_RS01940 at 395.044 kb on + strand, within BWI76_RS01940 at 395.321 kb on + strand at 395.347 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 392,840 + BWI76_RS01925 0.46 +0.5 392,860 + BWI76_RS01925 0.47 +1.1 392,988 - BWI76_RS01925 0.58 -1.6 393,187 + BWI76_RS01925 0.75 -0.1 393,212 - BWI76_RS01925 0.77 +0.0 393,263 + BWI76_RS01925 0.81 -0.2 393,273 + BWI76_RS01925 0.82 +0.3 393,280 - BWI76_RS01925 0.82 +0.1 393,281 + BWI76_RS01925 0.82 +0.1 393,369 + BWI76_RS01925 0.90 -0.8 393,371 - +0.1 393,371 - -2.9 393,371 - +1.5 393,390 + +0.7 393,457 - -0.8 393,457 - +0.6 393,463 + -4.5 393,484 + +0.3 393,493 - -1.4 393,504 + +0.3 393,512 - +0.0 393,531 + -1.6 393,531 + +0.9 393,531 + -0.8 393,539 - +0.4 393,583 - +1.4 393,699 + +0.9 393,706 + -0.7 393,823 - BWI76_RS01930 0.19 +0.0 393,823 - BWI76_RS01930 0.19 -0.7 393,838 - BWI76_RS01930 0.21 -2.1 394,227 + BWI76_RS01930 0.76 -1.8 394,285 + BWI76_RS01930 0.84 -2.7 394,369 + +0.5 394,377 - +0.2 394,473 + -1.9 394,488 - -1.1 394,560 - +0.6 394,637 + BWI76_RS01935 0.23 -1.3 394,858 - BWI76_RS01935 0.76 -0.4 394,862 - BWI76_RS01935 0.77 -2.5 394,934 - -0.6 394,994 + -0.8 395,004 + BWI76_RS01940 0.12 +0.5 395,009 + BWI76_RS01940 0.14 -0.7 395,044 + BWI76_RS01940 0.23 -1.2 395,321 + -0.1 395,347 - -4.2
Or see this region's nucleotide sequence