Experiment: KLEB17 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01055 and BWI76_RS01060 are separated by 18 nucleotides BWI76_RS01060 and BWI76_RS01065 are separated by 134 nucleotides
BWI76_RS01055: BWI76_RS01055 - LysR family transcriptional regulator, at 215,873 to 216,793
_RS01055
BWI76_RS01060: BWI76_RS01060 - guanosine-5'-triphosphate,3'-diphosphate pyrophosphatase, at 216,812 to 218,311
_RS01060
BWI76_RS01065: BWI76_RS01065 - ATP-dependent RNA helicase RhlB, at 218,446 to 219,711
_RS01065
Position (kb)
216
217
218
219 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1
2
3
4
5 at 215.819 kb on + strand at 215.825 kb on - strand at 215.825 kb on - strand at 215.886 kb on + strand at 215.888 kb on + strand at 215.888 kb on + strand at 215.894 kb on - strand at 215.896 kb on - strand at 215.897 kb on + strand at 215.901 kb on + strand at 215.903 kb on + strand at 215.916 kb on - strand at 215.923 kb on + strand at 215.923 kb on + strand at 215.931 kb on - strand at 215.931 kb on - strand at 215.931 kb on - strand at 215.933 kb on + strand at 215.933 kb on + strand at 215.933 kb on + strand at 215.941 kb on - strand at 215.941 kb on - strand at 215.941 kb on - strand at 215.941 kb on - strand at 215.951 kb on + strand at 215.953 kb on + strand at 215.958 kb on + strand at 215.964 kb on + strand at 215.964 kb on + strand at 215.964 kb on + strand at 215.972 kb on - strand, within BWI76_RS01055 at 215.972 kb on - strand, within BWI76_RS01055 at 215.977 kb on - strand, within BWI76_RS01055 at 216.009 kb on + strand, within BWI76_RS01055 at 216.017 kb on + strand, within BWI76_RS01055 at 216.036 kb on + strand, within BWI76_RS01055 at 216.044 kb on - strand, within BWI76_RS01055 at 216.073 kb on + strand, within BWI76_RS01055 at 216.113 kb on + strand, within BWI76_RS01055 at 216.121 kb on + strand, within BWI76_RS01055 at 216.121 kb on + strand, within BWI76_RS01055 at 216.121 kb on + strand, within BWI76_RS01055 at 216.122 kb on + strand, within BWI76_RS01055 at 216.129 kb on - strand, within BWI76_RS01055 at 216.129 kb on - strand, within BWI76_RS01055 at 216.129 kb on - strand, within BWI76_RS01055 at 216.129 kb on - strand, within BWI76_RS01055 at 216.129 kb on - strand, within BWI76_RS01055 at 216.129 kb on - strand, within BWI76_RS01055 at 216.130 kb on - strand, within BWI76_RS01055 at 216.130 kb on - strand, within BWI76_RS01055 at 216.176 kb on + strand, within BWI76_RS01055 at 216.181 kb on + strand, within BWI76_RS01055 at 216.184 kb on - strand, within BWI76_RS01055 at 216.184 kb on - strand, within BWI76_RS01055 at 216.184 kb on - strand, within BWI76_RS01055 at 216.184 kb on - strand, within BWI76_RS01055 at 216.189 kb on + strand, within BWI76_RS01055 at 216.191 kb on - strand, within BWI76_RS01055 at 216.227 kb on + strand, within BWI76_RS01055 at 216.335 kb on - strand, within BWI76_RS01055 at 216.367 kb on + strand, within BWI76_RS01055 at 216.390 kb on - strand, within BWI76_RS01055 at 216.445 kb on - strand, within BWI76_RS01055 at 216.743 kb on + strand at 216.869 kb on - strand at 217.032 kb on + strand, within BWI76_RS01060 at 217.032 kb on + strand, within BWI76_RS01060 at 217.227 kb on - strand, within BWI76_RS01060 at 217.430 kb on - strand, within BWI76_RS01060 at 217.434 kb on - strand, within BWI76_RS01060 at 217.435 kb on - strand, within BWI76_RS01060 at 217.442 kb on - strand, within BWI76_RS01060 at 217.717 kb on + strand, within BWI76_RS01060 at 217.720 kb on - strand, within BWI76_RS01060 at 217.835 kb on - strand, within BWI76_RS01060 at 217.993 kb on - strand, within BWI76_RS01060 at 218.297 kb on - strand at 218.299 kb on + strand at 218.321 kb on - strand at 218.332 kb on + strand at 218.340 kb on - strand at 218.448 kb on + strand at 218.451 kb on - strand at 218.533 kb on - strand at 218.533 kb on - strand at 218.965 kb on + strand, within BWI76_RS01065 at 218.966 kb on + strand, within BWI76_RS01065 at 219.065 kb on + strand, within BWI76_RS01065 at 219.124 kb on - strand, within BWI76_RS01065 at 219.124 kb on - strand, within BWI76_RS01065 at 219.254 kb on - strand, within BWI76_RS01065
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB17 MOI 1 remove 215,819 + +0.8 215,825 - -0.2 215,825 - +0.4 215,886 + -1.2 215,888 + +0.9 215,888 + -1.3 215,894 - -0.3 215,896 - -0.6 215,897 + -4.2 215,901 + +1.5 215,903 + -2.0 215,916 - -0.8 215,923 + -3.1 215,923 + +0.6 215,931 - +0.6 215,931 - +4.9 215,931 - -0.6 215,933 + -0.6 215,933 + -1.4 215,933 + -0.8 215,941 - -2.4 215,941 - +0.4 215,941 - +1.1 215,941 - +0.1 215,951 + -0.1 215,953 + -0.9 215,958 + -0.3 215,964 + -2.8 215,964 + +0.9 215,964 + -1.5 215,972 - BWI76_RS01055 0.11 +0.1 215,972 - BWI76_RS01055 0.11 -3.8 215,977 - BWI76_RS01055 0.11 -0.9 216,009 + BWI76_RS01055 0.15 -0.9 216,017 + BWI76_RS01055 0.16 +0.1 216,036 + BWI76_RS01055 0.18 -2.0 216,044 - BWI76_RS01055 0.19 +0.8 216,073 + BWI76_RS01055 0.22 -3.1 216,113 + BWI76_RS01055 0.26 +0.4 216,121 + BWI76_RS01055 0.27 +2.2 216,121 + BWI76_RS01055 0.27 +0.5 216,121 + BWI76_RS01055 0.27 +0.4 216,122 + BWI76_RS01055 0.27 -0.7 216,129 - BWI76_RS01055 0.28 -0.6 216,129 - BWI76_RS01055 0.28 -1.8 216,129 - BWI76_RS01055 0.28 -2.6 216,129 - BWI76_RS01055 0.28 -1.9 216,129 - BWI76_RS01055 0.28 -0.8 216,129 - BWI76_RS01055 0.28 -1.7 216,130 - BWI76_RS01055 0.28 -3.0 216,130 - BWI76_RS01055 0.28 -1.9 216,176 + BWI76_RS01055 0.33 -0.5 216,181 + BWI76_RS01055 0.33 +1.6 216,184 - BWI76_RS01055 0.34 -2.0 216,184 - BWI76_RS01055 0.34 -0.7 216,184 - BWI76_RS01055 0.34 -2.7 216,184 - BWI76_RS01055 0.34 -0.2 216,189 + BWI76_RS01055 0.34 -4.9 216,191 - BWI76_RS01055 0.35 +0.9 216,227 + BWI76_RS01055 0.38 -0.6 216,335 - BWI76_RS01055 0.50 -1.8 216,367 + BWI76_RS01055 0.54 -0.5 216,390 - BWI76_RS01055 0.56 +0.7 216,445 - BWI76_RS01055 0.62 +0.9 216,743 + -0.6 216,869 - -2.1 217,032 + BWI76_RS01060 0.15 -1.4 217,032 + BWI76_RS01060 0.15 -0.9 217,227 - BWI76_RS01060 0.28 -2.2 217,430 - BWI76_RS01060 0.41 -2.1 217,434 - BWI76_RS01060 0.41 -1.8 217,435 - BWI76_RS01060 0.42 -3.4 217,442 - BWI76_RS01060 0.42 -1.6 217,717 + BWI76_RS01060 0.60 -1.3 217,720 - BWI76_RS01060 0.61 -1.6 217,835 - BWI76_RS01060 0.68 -2.5 217,993 - BWI76_RS01060 0.79 -0.6 218,297 - +0.4 218,299 + -1.0 218,321 - -0.2 218,332 + +0.5 218,340 - +1.8 218,448 + -0.2 218,451 - +0.3 218,533 - +1.0 218,533 - -0.9 218,965 + BWI76_RS01065 0.41 -1.3 218,966 + BWI76_RS01065 0.41 -3.9 219,065 + BWI76_RS01065 0.49 -1.3 219,124 - BWI76_RS01065 0.54 -2.0 219,124 - BWI76_RS01065 0.54 -3.4 219,254 - BWI76_RS01065 0.64 -0.5
Or see this region's nucleotide sequence