Experiment: KLEB16 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS24305 and BWI76_RS24310 are separated by 94 nucleotides BWI76_RS24310 and BWI76_RS24315 are separated by 120 nucleotides
BWI76_RS24305: BWI76_RS24305 - 2,5-didehydrogluconate reductase A, at 5,063,725 to 5,064,552
_RS24305
BWI76_RS24310: BWI76_RS24310 - YgiQ family radical SAM protein, at 5,064,647 to 5,066,830
_RS24310
BWI76_RS24315: BWI76_RS24315 - cell division protein FtsP, at 5,066,951 to 5,068,363
_RS24315
Position (kb) 5064
5065
5066
5067 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 5063.666 kb on + strand at 5063.674 kb on - strand at 5063.710 kb on - strand at 5063.763 kb on + strand at 5063.783 kb on - strand at 5063.910 kb on - strand, within BWI76_RS24305 at 5063.912 kb on + strand, within BWI76_RS24305 at 5063.920 kb on - strand, within BWI76_RS24305 at 5063.976 kb on + strand, within BWI76_RS24305 at 5063.976 kb on + strand, within BWI76_RS24305 at 5064.083 kb on + strand, within BWI76_RS24305 at 5064.307 kb on + strand, within BWI76_RS24305 at 5064.385 kb on + strand, within BWI76_RS24305 at 5064.385 kb on + strand, within BWI76_RS24305 at 5064.385 kb on + strand, within BWI76_RS24305 at 5064.510 kb on + strand at 5064.906 kb on + strand, within BWI76_RS24310 at 5064.959 kb on + strand, within BWI76_RS24310 at 5064.969 kb on + strand, within BWI76_RS24310 at 5064.977 kb on - strand, within BWI76_RS24310 at 5064.993 kb on + strand, within BWI76_RS24310 at 5065.165 kb on + strand, within BWI76_RS24310 at 5065.345 kb on + strand, within BWI76_RS24310 at 5065.483 kb on - strand, within BWI76_RS24310 at 5065.517 kb on - strand, within BWI76_RS24310 at 5065.816 kb on + strand, within BWI76_RS24310 at 5065.925 kb on + strand, within BWI76_RS24310 at 5065.954 kb on + strand, within BWI76_RS24310 at 5066.077 kb on + strand, within BWI76_RS24310 at 5066.173 kb on - strand, within BWI76_RS24310 at 5066.400 kb on - strand, within BWI76_RS24310 at 5066.405 kb on - strand, within BWI76_RS24310 at 5066.479 kb on - strand, within BWI76_RS24310 at 5066.546 kb on + strand, within BWI76_RS24310 at 5066.561 kb on - strand, within BWI76_RS24310 at 5066.924 kb on + strand at 5066.939 kb on + strand at 5066.939 kb on + strand at 5066.985 kb on - strand at 5067.060 kb on - strand at 5067.255 kb on + strand, within BWI76_RS24315 at 5067.263 kb on - strand at 5067.554 kb on - strand, within BWI76_RS24315 at 5067.656 kb on - strand, within BWI76_RS24315 at 5067.767 kb on + strand, within BWI76_RS24315 at 5067.775 kb on - strand, within BWI76_RS24315
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 0.1 remove 5,063,666 + +0.2 5,063,674 - -0.1 5,063,710 - -0.7 5,063,763 + +0.1 5,063,783 - -0.1 5,063,910 - BWI76_RS24305 0.22 -0.4 5,063,912 + BWI76_RS24305 0.23 -0.9 5,063,920 - BWI76_RS24305 0.24 +0.4 5,063,976 + BWI76_RS24305 0.30 -1.0 5,063,976 + BWI76_RS24305 0.30 -0.8 5,064,083 + BWI76_RS24305 0.43 -0.3 5,064,307 + BWI76_RS24305 0.70 +0.2 5,064,385 + BWI76_RS24305 0.80 +1.0 5,064,385 + BWI76_RS24305 0.80 +0.4 5,064,385 + BWI76_RS24305 0.80 -4.1 5,064,510 + +0.9 5,064,906 + BWI76_RS24310 0.12 +0.0 5,064,959 + BWI76_RS24310 0.14 +0.6 5,064,969 + BWI76_RS24310 0.15 -2.9 5,064,977 - BWI76_RS24310 0.15 -0.0 5,064,993 + BWI76_RS24310 0.16 +0.7 5,065,165 + BWI76_RS24310 0.24 +0.1 5,065,345 + BWI76_RS24310 0.32 +1.3 5,065,483 - BWI76_RS24310 0.38 +0.9 5,065,517 - BWI76_RS24310 0.40 +0.7 5,065,816 + BWI76_RS24310 0.54 +0.4 5,065,925 + BWI76_RS24310 0.59 +0.4 5,065,954 + BWI76_RS24310 0.60 +0.9 5,066,077 + BWI76_RS24310 0.65 -0.1 5,066,173 - BWI76_RS24310 0.70 +0.1 5,066,400 - BWI76_RS24310 0.80 -0.3 5,066,405 - BWI76_RS24310 0.80 +4.2 5,066,479 - BWI76_RS24310 0.84 -0.7 5,066,546 + BWI76_RS24310 0.87 -0.4 5,066,561 - BWI76_RS24310 0.88 +0.1 5,066,924 + -0.5 5,066,939 + -0.2 5,066,939 + -0.3 5,066,985 - -0.3 5,067,060 - +0.4 5,067,255 + BWI76_RS24315 0.22 -0.7 5,067,263 - -0.2 5,067,554 - BWI76_RS24315 0.43 -0.9 5,067,656 - BWI76_RS24315 0.50 +0.1 5,067,767 + BWI76_RS24315 0.58 -0.4 5,067,775 - BWI76_RS24315 0.58 -0.8
Or see this region's nucleotide sequence