Experiment: KLEB16 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23120 and BWI76_RS23125 are separated by 14 nucleotides BWI76_RS23125 and BWI76_RS23130 are separated by 15 nucleotides BWI76_RS23130 and BWI76_RS23135 overlap by 11 nucleotides BWI76_RS23135 and BWI76_RS23140 are separated by 19 nucleotides BWI76_RS23140 and BWI76_RS23145 are separated by 24 nucleotides
BWI76_RS23120: BWI76_RS23120 - propanediol dehydratase medium subunit, at 4,820,386 to 4,821,045
_RS23120
BWI76_RS23125: BWI76_RS23125 - propanediol dehydratase small subunit, at 4,821,060 to 4,821,581
_RS23125
BWI76_RS23130: BWI76_RS23130 - diol dehydratase reactivase subunit alpha, at 4,821,597 to 4,823,429
_RS23130
BWI76_RS23135: BWI76_RS23135 - propanediol dehydratase, at 4,823,419 to 4,823,769
_RS23135
BWI76_RS23140: BWI76_RS23140 - carboxysome shell protein, at 4,823,789 to 4,824,064
_RS23140
BWI76_RS23145: BWI76_RS23145 - propanediol utilization protein, at 4,824,089 to 4,824,550
_RS23145
Position (kb) 4821
4822
4823
4824 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 4820.851 kb on + strand, within BWI76_RS23120 at 4820.920 kb on + strand, within BWI76_RS23120 at 4821.096 kb on + strand at 4821.290 kb on + strand, within BWI76_RS23125 at 4821.448 kb on - strand, within BWI76_RS23125 at 4821.582 kb on + strand at 4821.919 kb on + strand, within BWI76_RS23130 at 4821.951 kb on - strand, within BWI76_RS23130 at 4822.237 kb on - strand, within BWI76_RS23130 at 4822.237 kb on - strand, within BWI76_RS23130 at 4822.242 kb on + strand, within BWI76_RS23130 at 4822.466 kb on - strand, within BWI76_RS23130 at 4822.533 kb on + strand, within BWI76_RS23130 at 4822.538 kb on + strand at 4822.609 kb on + strand, within BWI76_RS23130 at 4822.646 kb on - strand, within BWI76_RS23130 at 4822.955 kb on - strand, within BWI76_RS23130 at 4823.457 kb on - strand, within BWI76_RS23135 at 4823.491 kb on + strand, within BWI76_RS23135 at 4823.559 kb on + strand, within BWI76_RS23135 at 4823.567 kb on - strand, within BWI76_RS23135 at 4823.914 kb on - strand, within BWI76_RS23140 at 4823.914 kb on - strand, within BWI76_RS23140 at 4824.082 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 0.1 remove 4,820,851 + BWI76_RS23120 0.70 -0.9 4,820,920 + BWI76_RS23120 0.81 +0.4 4,821,096 + -1.9 4,821,290 + BWI76_RS23125 0.44 -1.4 4,821,448 - BWI76_RS23125 0.74 -0.0 4,821,582 + +0.0 4,821,919 + BWI76_RS23130 0.18 -1.0 4,821,951 - BWI76_RS23130 0.19 -0.1 4,822,237 - BWI76_RS23130 0.35 -1.5 4,822,237 - BWI76_RS23130 0.35 -1.1 4,822,242 + BWI76_RS23130 0.35 -1.2 4,822,466 - BWI76_RS23130 0.47 -1.3 4,822,533 + BWI76_RS23130 0.51 -0.4 4,822,538 + -3.0 4,822,609 + BWI76_RS23130 0.55 -0.9 4,822,646 - BWI76_RS23130 0.57 -2.4 4,822,955 - BWI76_RS23130 0.74 -0.2 4,823,457 - BWI76_RS23135 0.11 +0.3 4,823,491 + BWI76_RS23135 0.21 -0.9 4,823,559 + BWI76_RS23135 0.40 -0.3 4,823,567 - BWI76_RS23135 0.42 -2.0 4,823,914 - BWI76_RS23140 0.45 +0.7 4,823,914 - BWI76_RS23140 0.45 +0.4 4,824,082 + -0.4
Or see this region's nucleotide sequence