Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS04650

Experiment: KLEB16 MOI 0.1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS04640 and BWI76_RS04645 are separated by 37 nucleotidesBWI76_RS04645 and BWI76_RS04650 are separated by 33 nucleotidesBWI76_RS04650 and BWI76_RS04655 are separated by 11 nucleotidesBWI76_RS04655 and BWI76_RS04660 are separated by 10 nucleotides BWI76_RS04640: BWI76_RS04640 - LysR family transcriptional regulator, at 966,808 to 967,704 _RS04640 BWI76_RS04645: BWI76_RS04645 - 3-dehydroquinase, at 967,742 to 968,497 _RS04645 BWI76_RS04650: BWI76_RS04650 - MFS transporter, at 968,531 to 969,745 _RS04650 BWI76_RS04655: BWI76_RS04655 - shikimate dehydrogenase, at 969,757 to 970,620 _RS04655 BWI76_RS04660: BWI76_RS04660 - 2,4-dienoyl-CoA reductase, at 970,631 to 972,664 _RS04660 Position (kb) 968 969 970Strain fitness (log2 ratio) -4 -3 -2 -1 0 1 2at 967.571 kb on + strand, within BWI76_RS04640at 967.610 kb on + strand, within BWI76_RS04640at 967.633 kb on - strandat 967.644 kb on + strandat 967.716 kb on + strandat 967.719 kb on - strandat 967.719 kb on - strandat 967.719 kb on - strandat 967.725 kb on - strandat 967.802 kb on + strandat 967.817 kb on + strandat 967.954 kb on - strand, within BWI76_RS04645at 967.954 kb on - strand, within BWI76_RS04645at 967.993 kb on + strand, within BWI76_RS04645at 968.047 kb on - strand, within BWI76_RS04645at 968.083 kb on - strand, within BWI76_RS04645at 968.114 kb on - strand, within BWI76_RS04645at 968.154 kb on - strand, within BWI76_RS04645at 968.174 kb on + strand, within BWI76_RS04645at 968.333 kb on - strand, within BWI76_RS04645at 968.426 kb on + strandat 968.481 kb on - strandat 968.738 kb on + strand, within BWI76_RS04650at 968.770 kb on - strand, within BWI76_RS04650at 968.794 kb on - strand, within BWI76_RS04650at 968.818 kb on - strand, within BWI76_RS04650at 968.990 kb on - strand, within BWI76_RS04650at 969.100 kb on + strand, within BWI76_RS04650at 969.100 kb on + strand, within BWI76_RS04650at 969.108 kb on - strand, within BWI76_RS04650at 969.137 kb on - strand, within BWI76_RS04650at 969.272 kb on + strand, within BWI76_RS04650at 969.347 kb on - strand, within BWI76_RS04650at 969.347 kb on - strand, within BWI76_RS04650at 969.347 kb on - strand, within BWI76_RS04650at 969.352 kb on + strand, within BWI76_RS04650at 969.662 kb on - strandat 969.778 kb on - strandat 969.825 kb on + strandat 969.825 kb on + strandat 969.921 kb on + strand, within BWI76_RS04655at 970.025 kb on - strandat 970.287 kb on - strand, within BWI76_RS04655at 970.356 kb on + strand, within BWI76_RS04655at 970.458 kb on - strand, within BWI76_RS04655at 970.545 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB16 MOI 0.1
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967,571 + BWI76_RS04640 0.85 -0.3
967,610 + BWI76_RS04640 0.89 +0.3
967,633 - -0.5
967,644 + +0.5
967,716 + -0.7
967,719 - +0.5
967,719 - -2.0
967,719 - +0.8
967,725 - +0.6
967,802 + -1.3
967,817 + +1.0
967,954 - BWI76_RS04645 0.28 -3.9
967,954 - BWI76_RS04645 0.28 +0.0
967,993 + BWI76_RS04645 0.33 +0.2
968,047 - BWI76_RS04645 0.40 -0.5
968,083 - BWI76_RS04645 0.45 -1.3
968,114 - BWI76_RS04645 0.49 -0.5
968,154 - BWI76_RS04645 0.54 -1.0
968,174 + BWI76_RS04645 0.57 -1.0
968,333 - BWI76_RS04645 0.78 -0.3
968,426 + -1.3
968,481 - -1.0
968,738 + BWI76_RS04650 0.17 -0.4
968,770 - BWI76_RS04650 0.20 +0.5
968,794 - BWI76_RS04650 0.22 -1.4
968,818 - BWI76_RS04650 0.24 +0.1
968,990 - BWI76_RS04650 0.38 +0.3
969,100 + BWI76_RS04650 0.47 -1.0
969,100 + BWI76_RS04650 0.47 +0.3
969,108 - BWI76_RS04650 0.47 +0.6
969,137 - BWI76_RS04650 0.50 +0.2
969,272 + BWI76_RS04650 0.61 +0.6
969,347 - BWI76_RS04650 0.67 +1.6
969,347 - BWI76_RS04650 0.67 +2.2
969,347 - BWI76_RS04650 0.67 +0.5
969,352 + BWI76_RS04650 0.68 -0.6
969,662 - +0.1
969,778 - -0.4
969,825 + +0.8
969,825 + -2.4
969,921 + BWI76_RS04655 0.19 -0.2
970,025 - +0.6
970,287 - BWI76_RS04655 0.61 -1.5
970,356 + BWI76_RS04655 0.69 +1.2
970,458 - BWI76_RS04655 0.81 +0.3
970,545 - +0.9

Or see this region's nucleotide sequence