Experiment: KLEB16 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14345 and BWI76_RS14350 are separated by 235 nucleotides BWI76_RS14350 and BWI76_RS14355 are separated by 294 nucleotides BWI76_RS14355 and BWI76_RS14360 are separated by 391 nucleotides
BWI76_RS14345: BWI76_RS14345 - hypothetical protein, at 2,997,377 to 3,000,175
_RS14345
BWI76_RS14350: BWI76_RS14350 - hypothetical protein, at 3,000,411 to 3,000,611
_RS14350
BWI76_RS14355: BWI76_RS14355 - Low calcium response locus protein T, at 3,000,906 to 3,000,980
_RS14355
BWI76_RS14360: BWI76_RS14360 - hypothetical protein, at 3,001,372 to 3,001,932
_RS14360
Position (kb)
3000
3001 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2999.994 kb on - strand at 3000.024 kb on - strand at 3000.271 kb on + strand at 3000.350 kb on - strand at 3000.376 kb on + strand at 3000.445 kb on - strand, within BWI76_RS14350 at 3000.494 kb on + strand, within BWI76_RS14350 at 3000.503 kb on + strand, within BWI76_RS14350 at 3000.536 kb on + strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.544 kb on - strand, within BWI76_RS14350 at 3000.580 kb on - strand, within BWI76_RS14350 at 3000.660 kb on - strand at 3000.722 kb on - strand at 3000.758 kb on + strand at 3000.813 kb on + strand at 3000.821 kb on - strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.844 kb on + strand at 3000.852 kb on - strand at 3000.852 kb on - strand at 3000.867 kb on - strand at 3000.881 kb on - strand at 3000.887 kb on + strand at 3000.895 kb on - strand at 3000.926 kb on + strand, within BWI76_RS14355 at 3000.953 kb on + strand, within BWI76_RS14355 at 3001.015 kb on - strand at 3001.048 kb on + strand at 3001.070 kb on + strand at 3001.093 kb on + strand at 3001.095 kb on + strand at 3001.095 kb on + strand at 3001.096 kb on + strand at 3001.104 kb on - strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.106 kb on + strand at 3001.114 kb on - strand at 3001.114 kb on - strand at 3001.123 kb on + strand at 3001.129 kb on + strand at 3001.135 kb on + strand at 3001.142 kb on + strand at 3001.142 kb on + strand at 3001.145 kb on - strand at 3001.183 kb on + strand at 3001.183 kb on + strand at 3001.183 kb on + strand at 3001.183 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.245 kb on + strand at 3001.247 kb on + strand at 3001.247 kb on + strand at 3001.252 kb on + strand at 3001.253 kb on - strand at 3001.253 kb on - strand at 3001.255 kb on - strand at 3001.282 kb on + strand at 3001.284 kb on + strand at 3001.284 kb on + strand at 3001.295 kb on + strand at 3001.312 kb on + strand at 3001.320 kb on - strand at 3001.344 kb on + strand at 3001.463 kb on - strand, within BWI76_RS14360 at 3001.468 kb on - strand, within BWI76_RS14360 at 3001.500 kb on - strand, within BWI76_RS14360 at 3001.905 kb on + strand at 3001.913 kb on - strand at 3001.976 kb on - strand at 3001.976 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 0.1 remove 2,999,994 - -0.4 3,000,024 - +1.0 3,000,271 + +0.2 3,000,350 - +0.7 3,000,376 + +0.1 3,000,445 - BWI76_RS14350 0.17 -0.2 3,000,494 + BWI76_RS14350 0.41 -0.6 3,000,503 + BWI76_RS14350 0.46 -1.4 3,000,536 + BWI76_RS14350 0.62 +0.2 3,000,544 - BWI76_RS14350 0.66 +0.4 3,000,544 - BWI76_RS14350 0.66 -0.5 3,000,544 - BWI76_RS14350 0.66 -1.2 3,000,544 - BWI76_RS14350 0.66 +1.3 3,000,580 - BWI76_RS14350 0.84 +0.4 3,000,660 - +1.1 3,000,722 - +1.4 3,000,758 + -2.3 3,000,813 + -2.2 3,000,821 - +0.4 3,000,844 + +0.1 3,000,844 + -0.6 3,000,844 + +0.4 3,000,844 + -0.6 3,000,852 - -0.5 3,000,852 - -0.0 3,000,867 - -1.5 3,000,881 - -2.7 3,000,887 + -0.3 3,000,895 - +1.4 3,000,926 + BWI76_RS14355 0.27 +0.7 3,000,953 + BWI76_RS14355 0.63 +0.2 3,001,015 - +2.0 3,001,048 + -1.0 3,001,070 + +1.2 3,001,093 + +0.4 3,001,095 + +0.1 3,001,095 + -0.3 3,001,096 + +0.4 3,001,104 - -0.4 3,001,106 + -1.4 3,001,106 + +0.3 3,001,106 + +0.1 3,001,106 + -2.0 3,001,114 - +1.6 3,001,114 - -0.2 3,001,123 + +0.0 3,001,129 + +2.4 3,001,135 + -0.5 3,001,142 + -1.9 3,001,142 + -1.5 3,001,145 - -0.6 3,001,183 + -0.6 3,001,183 + -1.1 3,001,183 + -0.4 3,001,183 + +1.4 3,001,245 + -3.1 3,001,245 + -0.2 3,001,245 + +2.2 3,001,245 + -0.3 3,001,245 + -0.7 3,001,245 + -1.0 3,001,245 + -0.4 3,001,245 + -1.9 3,001,245 + -0.9 3,001,245 + +0.6 3,001,245 + -0.1 3,001,245 + -1.2 3,001,247 + +0.2 3,001,247 + +0.6 3,001,252 + -1.7 3,001,253 - -0.3 3,001,253 - +1.4 3,001,255 - +1.4 3,001,282 + +0.1 3,001,284 + +0.5 3,001,284 + -0.2 3,001,295 + -0.9 3,001,312 + -1.4 3,001,320 - -2.2 3,001,344 + +1.4 3,001,463 - BWI76_RS14360 0.16 -1.2 3,001,468 - BWI76_RS14360 0.17 +1.4 3,001,500 - BWI76_RS14360 0.23 -2.3 3,001,905 + +0.4 3,001,913 - -0.2 3,001,976 - -0.4 3,001,976 - +1.4
Or see this region's nucleotide sequence