Experiment: KLEB16 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22650 and BWI76_RS22655 are separated by 122 nucleotides BWI76_RS22655 and BWI76_RS22660 overlap by 7 nucleotides BWI76_RS22660 and BWI76_RS22665 overlap by 20 nucleotides
BWI76_RS22650: BWI76_RS22650 - lipoprotein NlpD, at 4,721,254 to 4,722,372
_RS22650
BWI76_RS22655: BWI76_RS22655 - protein-L-isoaspartate O-methyltransferase, at 4,722,495 to 4,723,121
_RS22655
BWI76_RS22660: BWI76_RS22660 - 5'/3'-nucleotidase SurE, at 4,723,115 to 4,723,876
_RS22660
BWI76_RS22665: BWI76_RS22665 - tRNA pseudouridine(13) synthase TruD, at 4,723,857 to 4,724,906
_RS22665
Position (kb)
4723
4724 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 4722.199 kb on + strand, within BWI76_RS22650 at 4722.242 kb on + strand, within BWI76_RS22650 at 4722.308 kb on - strand at 4722.378 kb on - strand at 4722.421 kb on + strand at 4722.775 kb on - strand, within BWI76_RS22655 at 4722.799 kb on + strand, within BWI76_RS22655 at 4722.807 kb on - strand, within BWI76_RS22655 at 4722.807 kb on - strand, within BWI76_RS22655 at 4722.901 kb on - strand, within BWI76_RS22655 at 4722.933 kb on + strand, within BWI76_RS22655 at 4722.941 kb on - strand at 4723.281 kb on + strand, within BWI76_RS22660 at 4723.289 kb on - strand, within BWI76_RS22660 at 4723.368 kb on + strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.376 kb on - strand, within BWI76_RS22660 at 4723.463 kb on + strand, within BWI76_RS22660 at 4723.637 kb on + strand, within BWI76_RS22660 at 4723.657 kb on + strand, within BWI76_RS22660 at 4723.665 kb on - strand, within BWI76_RS22660 at 4723.685 kb on - strand, within BWI76_RS22660 at 4723.831 kb on - strand at 4724.108 kb on - strand, within BWI76_RS22665 at 4724.504 kb on + strand, within BWI76_RS22665 at 4724.504 kb on + strand, within BWI76_RS22665 at 4724.512 kb on - strand, within BWI76_RS22665 at 4724.614 kb on - strand, within BWI76_RS22665 at 4724.841 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 1 remove 4,722,199 + BWI76_RS22650 0.84 +1.6 4,722,242 + BWI76_RS22650 0.88 +3.8 4,722,308 - +3.5 4,722,378 - +0.7 4,722,421 + -0.4 4,722,775 - BWI76_RS22655 0.45 -0.0 4,722,799 + BWI76_RS22655 0.48 -0.5 4,722,807 - BWI76_RS22655 0.50 -0.1 4,722,807 - BWI76_RS22655 0.50 +0.0 4,722,901 - BWI76_RS22655 0.65 +0.3 4,722,933 + BWI76_RS22655 0.70 +1.9 4,722,941 - +0.6 4,723,281 + BWI76_RS22660 0.22 +0.5 4,723,289 - BWI76_RS22660 0.23 -1.8 4,723,368 + BWI76_RS22660 0.33 -0.5 4,723,376 - BWI76_RS22660 0.34 +0.3 4,723,376 - BWI76_RS22660 0.34 -0.2 4,723,376 - BWI76_RS22660 0.34 +0.5 4,723,463 + BWI76_RS22660 0.46 +0.7 4,723,637 + BWI76_RS22660 0.69 +1.4 4,723,657 + BWI76_RS22660 0.71 +0.2 4,723,665 - BWI76_RS22660 0.72 -0.0 4,723,685 - BWI76_RS22660 0.75 +0.3 4,723,831 - +1.4 4,724,108 - BWI76_RS22665 0.24 +2.5 4,724,504 + BWI76_RS22665 0.62 +0.8 4,724,504 + BWI76_RS22665 0.62 +0.5 4,724,512 - BWI76_RS22665 0.62 -0.2 4,724,614 - BWI76_RS22665 0.72 -0.4 4,724,841 + -0.1
Or see this region's nucleotide sequence