Experiment: KLEB16 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22460 and BWI76_RS22465 overlap by 4 nucleotides BWI76_RS22465 and BWI76_RS22470 overlap by 1 nucleotides BWI76_RS22470 and BWI76_RS22475 overlap by 8 nucleotides BWI76_RS22475 and BWI76_RS22480 are separated by 96 nucleotides
BWI76_RS22460: BWI76_RS22460 - hemin-degrading factor, at 4,683,073 to 4,684,113
_RS22460
BWI76_RS22465: BWI76_RS22465 - hemin ABC transporter substrate-binding protein, at 4,684,110 to 4,684,928
_RS22465
BWI76_RS22470: BWI76_RS22470 - transporter permease, at 4,684,928 to 4,685,923
_RS22470
BWI76_RS22475: BWI76_RS22475 - heme ABC transporter ATP-binding protein, at 4,685,916 to 4,686,695
_RS22475
BWI76_RS22480: BWI76_RS22480 - lactaldehyde reductase, at 4,686,792 to 4,687,943
_RS22480
Position (kb) 4684
4685
4686 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 4683.947 kb on - strand, within BWI76_RS22460 at 4684.023 kb on + strand at 4684.030 kb on + strand at 4684.031 kb on - strand at 4684.031 kb on - strand at 4684.067 kb on + strand at 4684.280 kb on - strand, within BWI76_RS22465 at 4684.360 kb on - strand at 4684.406 kb on + strand, within BWI76_RS22465 at 4684.519 kb on + strand, within BWI76_RS22465 at 4684.521 kb on - strand, within BWI76_RS22465 at 4684.663 kb on + strand, within BWI76_RS22465 at 4684.810 kb on - strand, within BWI76_RS22465 at 4684.872 kb on - strand at 4685.003 kb on - strand at 4685.172 kb on - strand, within BWI76_RS22470 at 4685.404 kb on + strand, within BWI76_RS22470 at 4685.513 kb on + strand, within BWI76_RS22470 at 4685.798 kb on + strand, within BWI76_RS22470 at 4686.110 kb on - strand, within BWI76_RS22475 at 4686.228 kb on + strand, within BWI76_RS22475 at 4686.468 kb on + strand, within BWI76_RS22475 at 4686.530 kb on + strand, within BWI76_RS22475 at 4686.576 kb on + strand, within BWI76_RS22475 at 4686.638 kb on + strand at 4686.654 kb on + strand at 4686.682 kb on + strand at 4686.685 kb on - strand at 4686.690 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 1 remove 4,683,947 - BWI76_RS22460 0.84 -0.9 4,684,023 + -0.6 4,684,030 + -0.6 4,684,031 - -0.7 4,684,031 - +1.0 4,684,067 + -0.4 4,684,280 - BWI76_RS22465 0.21 +0.3 4,684,360 - -1.1 4,684,406 + BWI76_RS22465 0.36 +0.6 4,684,519 + BWI76_RS22465 0.50 -0.0 4,684,521 - BWI76_RS22465 0.50 +0.4 4,684,663 + BWI76_RS22465 0.68 -0.2 4,684,810 - BWI76_RS22465 0.85 -0.1 4,684,872 - -0.4 4,685,003 - -0.0 4,685,172 - BWI76_RS22470 0.24 +0.1 4,685,404 + BWI76_RS22470 0.48 +1.6 4,685,513 + BWI76_RS22470 0.59 +1.6 4,685,798 + BWI76_RS22470 0.87 +1.3 4,686,110 - BWI76_RS22475 0.25 +0.8 4,686,228 + BWI76_RS22475 0.40 -3.0 4,686,468 + BWI76_RS22475 0.71 +0.3 4,686,530 + BWI76_RS22475 0.79 +2.9 4,686,576 + BWI76_RS22475 0.85 -0.7 4,686,638 + +0.3 4,686,654 + +0.1 4,686,682 + +1.3 4,686,685 - -1.1 4,686,690 - +0.3
Or see this region's nucleotide sequence