Experiment: KLEB16 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS22350 and BWI76_RS22355 overlap by 10 nucleotides BWI76_RS22355 and BWI76_RS22360 are separated by 0 nucleotides BWI76_RS22360 and BWI76_RS22365 overlap by 4 nucleotides
BWI76_RS22350: BWI76_RS22350 - hydrogenase nickel incorporation protein HypB, at 4,658,330 to 4,659,202
_RS22350
BWI76_RS22355: BWI76_RS22355 - hydrogenase assembly chaperone, at 4,659,193 to 4,659,465
_RS22355
BWI76_RS22360: BWI76_RS22360 - hydrogenase formation protein HypD, at 4,659,466 to 4,660,587
_RS22360
BWI76_RS22365: BWI76_RS22365 - hydrogenase expression/formation protein HypE, at 4,660,584 to 4,661,594
_RS22365
Position (kb)
4659
4660
4661 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4658.470 kb on + strand, within BWI76_RS22350 at 4658.478 kb on - strand, within BWI76_RS22350 at 4658.745 kb on - strand, within BWI76_RS22350 at 4658.745 kb on - strand, within BWI76_RS22350 at 4658.950 kb on + strand, within BWI76_RS22350 at 4659.156 kb on + strand at 4659.163 kb on - strand at 4659.254 kb on - strand, within BWI76_RS22355 at 4659.334 kb on + strand, within BWI76_RS22355 at 4659.936 kb on - strand, within BWI76_RS22360 at 4660.068 kb on + strand, within BWI76_RS22360 at 4660.101 kb on - strand, within BWI76_RS22360 at 4660.135 kb on - strand, within BWI76_RS22360 at 4660.196 kb on - strand, within BWI76_RS22360 at 4660.407 kb on + strand, within BWI76_RS22360 at 4660.615 kb on - strand at 4660.678 kb on - strand at 4661.187 kb on + strand, within BWI76_RS22365 at 4661.235 kb on + strand, within BWI76_RS22365 at 4661.416 kb on - strand, within BWI76_RS22365 at 4661.524 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 1 remove 4,658,470 + BWI76_RS22350 0.16 +1.9 4,658,478 - BWI76_RS22350 0.17 -0.9 4,658,745 - BWI76_RS22350 0.48 -0.6 4,658,745 - BWI76_RS22350 0.48 -0.6 4,658,950 + BWI76_RS22350 0.71 -1.2 4,659,156 + -0.5 4,659,163 - +0.3 4,659,254 - BWI76_RS22355 0.22 +1.1 4,659,334 + BWI76_RS22355 0.52 +0.5 4,659,936 - BWI76_RS22360 0.42 +0.0 4,660,068 + BWI76_RS22360 0.54 -1.0 4,660,101 - BWI76_RS22360 0.57 -0.8 4,660,135 - BWI76_RS22360 0.60 -1.3 4,660,196 - BWI76_RS22360 0.65 -2.0 4,660,407 + BWI76_RS22360 0.84 -0.6 4,660,615 - -0.3 4,660,678 - +0.8 4,661,187 + BWI76_RS22365 0.60 -0.1 4,661,235 + BWI76_RS22365 0.64 -0.5 4,661,416 - BWI76_RS22365 0.82 +0.1 4,661,524 + +1.2
Or see this region's nucleotide sequence