Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS22360

Experiment: KLEB16 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS22350 and BWI76_RS22355 overlap by 10 nucleotidesBWI76_RS22355 and BWI76_RS22360 are separated by 0 nucleotidesBWI76_RS22360 and BWI76_RS22365 overlap by 4 nucleotides BWI76_RS22350: BWI76_RS22350 - hydrogenase nickel incorporation protein HypB, at 4,658,330 to 4,659,202 _RS22350 BWI76_RS22355: BWI76_RS22355 - hydrogenase assembly chaperone, at 4,659,193 to 4,659,465 _RS22355 BWI76_RS22360: BWI76_RS22360 - hydrogenase formation protein HypD, at 4,659,466 to 4,660,587 _RS22360 BWI76_RS22365: BWI76_RS22365 - hydrogenase expression/formation protein HypE, at 4,660,584 to 4,661,594 _RS22365 Position (kb) 4659 4660 4661Strain fitness (log2 ratio) -2 -1 0 1 2at 4658.470 kb on + strand, within BWI76_RS22350at 4658.478 kb on - strand, within BWI76_RS22350at 4658.745 kb on - strand, within BWI76_RS22350at 4658.745 kb on - strand, within BWI76_RS22350at 4658.950 kb on + strand, within BWI76_RS22350at 4659.156 kb on + strandat 4659.163 kb on - strandat 4659.254 kb on - strand, within BWI76_RS22355at 4659.334 kb on + strand, within BWI76_RS22355at 4659.936 kb on - strand, within BWI76_RS22360at 4660.068 kb on + strand, within BWI76_RS22360at 4660.101 kb on - strand, within BWI76_RS22360at 4660.135 kb on - strand, within BWI76_RS22360at 4660.196 kb on - strand, within BWI76_RS22360at 4660.407 kb on + strand, within BWI76_RS22360at 4660.615 kb on - strandat 4660.678 kb on - strandat 4661.187 kb on + strand, within BWI76_RS22365at 4661.235 kb on + strand, within BWI76_RS22365at 4661.416 kb on - strand, within BWI76_RS22365at 4661.524 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB16 MOI 1
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4,658,470 + BWI76_RS22350 0.16 +1.9
4,658,478 - BWI76_RS22350 0.17 -0.9
4,658,745 - BWI76_RS22350 0.48 -0.6
4,658,745 - BWI76_RS22350 0.48 -0.6
4,658,950 + BWI76_RS22350 0.71 -1.2
4,659,156 + -0.5
4,659,163 - +0.3
4,659,254 - BWI76_RS22355 0.22 +1.1
4,659,334 + BWI76_RS22355 0.52 +0.5
4,659,936 - BWI76_RS22360 0.42 +0.0
4,660,068 + BWI76_RS22360 0.54 -1.0
4,660,101 - BWI76_RS22360 0.57 -0.8
4,660,135 - BWI76_RS22360 0.60 -1.3
4,660,196 - BWI76_RS22360 0.65 -2.0
4,660,407 + BWI76_RS22360 0.84 -0.6
4,660,615 - -0.3
4,660,678 - +0.8
4,661,187 + BWI76_RS22365 0.60 -0.1
4,661,235 + BWI76_RS22365 0.64 -0.5
4,661,416 - BWI76_RS22365 0.82 +0.1
4,661,524 + +1.2

Or see this region's nucleotide sequence