Experiment: KLEB16 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS17925 and BWI76_RS17930 are separated by 190 nucleotides BWI76_RS17930 and BWI76_RS17935 are separated by 41 nucleotides BWI76_RS17935 and BWI76_RS17940 overlap by 39 nucleotides BWI76_RS17940 and BWI76_RS17945 are separated by 461 nucleotides BWI76_RS17945 and BWI76_RS17950 are separated by 74 nucleotides
BWI76_RS17925: BWI76_RS17925 - peptidoglycan synthase, at 3,726,634 to 3,728,379
_RS17925
BWI76_RS17930: BWI76_RS17930 - cold-shock protein CspC, at 3,728,570 to 3,728,779
_RS17930
BWI76_RS17935: BWI76_RS17935 - DUF2627 domain-containing protein, at 3,728,821 to 3,728,937
_RS17935
BWI76_RS17940: BWI76_RS17940 - hypothetical protein, at 3,728,899 to 3,729,093
_RS17940
BWI76_RS17945: BWI76_RS17945 - hypothetical protein, at 3,729,555 to 3,729,845
_RS17945
BWI76_RS17950: BWI76_RS17950 - PhoP regulon feedback inhibition membrane protein MgrB, at 3,729,920 to 3,730,063
_RS17950
Position (kb)
3728
3729
3730 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3727.943 kb on - strand, within BWI76_RS17925 at 3727.967 kb on + strand, within BWI76_RS17925 at 3728.292 kb on + strand at 3728.333 kb on - strand at 3728.606 kb on - strand, within BWI76_RS17930 at 3728.684 kb on - strand, within BWI76_RS17930 at 3728.687 kb on - strand, within BWI76_RS17930 at 3728.733 kb on + strand, within BWI76_RS17930 at 3728.741 kb on - strand, within BWI76_RS17930 at 3728.763 kb on + strand at 3728.771 kb on - strand at 3728.771 kb on - strand at 3728.771 kb on - strand at 3728.797 kb on - strand at 3728.826 kb on - strand at 3728.860 kb on + strand, within BWI76_RS17935 at 3728.868 kb on - strand, within BWI76_RS17935 at 3728.868 kb on - strand, within BWI76_RS17935 at 3728.913 kb on - strand at 3728.943 kb on + strand, within BWI76_RS17940 at 3728.943 kb on - strand, within BWI76_RS17940 at 3728.946 kb on - strand, within BWI76_RS17940 at 3728.951 kb on - strand, within BWI76_RS17940 at 3728.951 kb on - strand, within BWI76_RS17940 at 3728.954 kb on - strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.014 kb on + strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.022 kb on - strand, within BWI76_RS17940 at 3729.041 kb on - strand, within BWI76_RS17940 at 3729.057 kb on + strand, within BWI76_RS17940 at 3729.156 kb on - strand at 3729.185 kb on - strand at 3729.202 kb on + strand at 3729.213 kb on + strand at 3729.235 kb on - strand at 3729.317 kb on + strand at 3729.317 kb on + strand at 3729.325 kb on - strand at 3729.352 kb on + strand at 3729.362 kb on + strand at 3729.362 kb on + strand at 3729.365 kb on + strand at 3729.370 kb on - strand at 3729.370 kb on - strand at 3729.388 kb on - strand at 3729.442 kb on + strand at 3729.442 kb on + strand at 3729.512 kb on + strand at 3729.527 kb on + strand at 3729.527 kb on + strand at 3729.527 kb on - strand at 3729.535 kb on - strand at 3729.542 kb on + strand at 3729.553 kb on + strand at 3729.553 kb on + strand at 3729.553 kb on + strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.561 kb on - strand at 3729.780 kb on - strand, within BWI76_RS17945 at 3729.805 kb on - strand, within BWI76_RS17945 at 3729.915 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 1 remove 3,727,943 - BWI76_RS17925 0.75 +1.4 3,727,967 + BWI76_RS17925 0.76 +0.6 3,728,292 + +2.4 3,728,333 - +0.1 3,728,606 - BWI76_RS17930 0.17 +0.4 3,728,684 - BWI76_RS17930 0.54 +0.7 3,728,687 - BWI76_RS17930 0.56 -0.4 3,728,733 + BWI76_RS17930 0.78 +4.3 3,728,741 - BWI76_RS17930 0.81 +0.8 3,728,763 + +0.1 3,728,771 - +0.7 3,728,771 - +1.2 3,728,771 - +1.7 3,728,797 - +1.2 3,728,826 - +1.3 3,728,860 + BWI76_RS17935 0.33 +1.1 3,728,868 - BWI76_RS17935 0.40 +2.1 3,728,868 - BWI76_RS17935 0.40 -0.4 3,728,913 - +1.3 3,728,943 + BWI76_RS17940 0.23 -0.3 3,728,943 - BWI76_RS17940 0.23 +0.9 3,728,946 - BWI76_RS17940 0.24 +0.6 3,728,951 - BWI76_RS17940 0.27 +0.3 3,728,951 - BWI76_RS17940 0.27 +0.8 3,728,954 - BWI76_RS17940 0.28 +0.7 3,729,014 + BWI76_RS17940 0.59 +2.0 3,729,014 + BWI76_RS17940 0.59 +0.8 3,729,014 + BWI76_RS17940 0.59 +2.0 3,729,014 + BWI76_RS17940 0.59 -0.2 3,729,014 + -1.3 3,729,014 + BWI76_RS17940 0.59 +3.7 3,729,014 + BWI76_RS17940 0.59 -0.9 3,729,022 - BWI76_RS17940 0.63 -0.1 3,729,022 - BWI76_RS17940 0.63 +0.6 3,729,022 - BWI76_RS17940 0.63 -0.0 3,729,022 - BWI76_RS17940 0.63 -0.4 3,729,022 - BWI76_RS17940 0.63 +0.5 3,729,022 - BWI76_RS17940 0.63 +1.1 3,729,041 - BWI76_RS17940 0.73 +1.2 3,729,057 + BWI76_RS17940 0.81 +2.2 3,729,156 - -1.3 3,729,185 - +0.1 3,729,202 + -1.2 3,729,213 + +0.7 3,729,235 - +1.7 3,729,317 + -1.3 3,729,317 + +1.7 3,729,325 - +0.6 3,729,352 + +0.3 3,729,362 + -0.1 3,729,362 + +0.4 3,729,365 + +0.1 3,729,370 - +0.0 3,729,370 - +2.2 3,729,388 - -1.6 3,729,442 + -0.3 3,729,442 + -0.6 3,729,512 + -0.4 3,729,527 + +0.6 3,729,527 + +1.7 3,729,527 - +0.5 3,729,535 - -2.1 3,729,542 + +0.9 3,729,553 + -0.3 3,729,553 + +1.7 3,729,553 + +1.7 3,729,561 - +0.2 3,729,561 - +0.9 3,729,561 - -1.3 3,729,561 - +0.7 3,729,561 - -0.1 3,729,561 - +0.7 3,729,561 - -0.0 3,729,780 - BWI76_RS17945 0.77 +0.3 3,729,805 - BWI76_RS17945 0.86 -2.6 3,729,915 - +0.9
Or see this region's nucleotide sequence