Experiment: KLEB16 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS11050 and BWI76_RS11055 are separated by 12 nucleotides BWI76_RS11055 and BWI76_RS11060 are separated by 190 nucleotides BWI76_RS11060 and BWI76_RS11065 are separated by 141 nucleotides
BWI76_RS11050: BWI76_RS11050 - 23S rRNA pseudouridine(955/2504/2580) synthase, at 2,332,413 to 2,333,408
_RS11050
BWI76_RS11055: BWI76_RS11055 - hydrolase, at 2,333,421 to 2,334,566
_RS11055
BWI76_RS11060: BWI76_RS11060 - septum formation inhibitor Maf, at 2,334,757 to 2,335,341
_RS11060
BWI76_RS11065: BWI76_RS11065 - hypothetical protein, at 2,335,483 to 2,336,004
_RS11065
Position (kb)
2333
2334
2335 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 2332.541 kb on + strand, within BWI76_RS11050 at 2332.547 kb on - strand, within BWI76_RS11050 at 2332.547 kb on - strand, within BWI76_RS11050 at 2332.549 kb on - strand, within BWI76_RS11050 at 2332.549 kb on - strand, within BWI76_RS11050 at 2332.585 kb on + strand, within BWI76_RS11050 at 2332.699 kb on + strand, within BWI76_RS11050 at 2332.806 kb on + strand, within BWI76_RS11050 at 2332.813 kb on + strand, within BWI76_RS11050 at 2332.821 kb on - strand, within BWI76_RS11050 at 2332.989 kb on - strand, within BWI76_RS11050 at 2333.186 kb on - strand, within BWI76_RS11050 at 2333.211 kb on - strand, within BWI76_RS11050 at 2333.570 kb on + strand, within BWI76_RS11055 at 2333.838 kb on + strand, within BWI76_RS11055 at 2334.197 kb on + strand, within BWI76_RS11055 at 2334.286 kb on - strand, within BWI76_RS11055 at 2334.373 kb on - strand, within BWI76_RS11055 at 2334.548 kb on - strand at 2334.685 kb on + strand at 2334.914 kb on - strand, within BWI76_RS11060 at 2335.003 kb on - strand at 2335.153 kb on + strand, within BWI76_RS11060
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 1 remove 2,332,541 + BWI76_RS11050 0.13 -2.5 2,332,547 - BWI76_RS11050 0.13 -2.8 2,332,547 - BWI76_RS11050 0.13 -3.0 2,332,549 - BWI76_RS11050 0.14 -0.4 2,332,549 - BWI76_RS11050 0.14 -1.3 2,332,585 + BWI76_RS11050 0.17 -0.1 2,332,699 + BWI76_RS11050 0.29 +0.4 2,332,806 + BWI76_RS11050 0.39 +0.5 2,332,813 + BWI76_RS11050 0.40 -3.9 2,332,821 - BWI76_RS11050 0.41 +0.4 2,332,989 - BWI76_RS11050 0.58 +3.0 2,333,186 - BWI76_RS11050 0.78 +0.7 2,333,211 - BWI76_RS11050 0.80 -2.3 2,333,570 + BWI76_RS11055 0.13 +0.4 2,333,838 + BWI76_RS11055 0.36 -0.1 2,334,197 + BWI76_RS11055 0.68 +1.0 2,334,286 - BWI76_RS11055 0.75 +0.8 2,334,373 - BWI76_RS11055 0.83 +1.3 2,334,548 - +0.5 2,334,685 + +0.9 2,334,914 - BWI76_RS11060 0.27 +1.1 2,335,003 - -1.1 2,335,153 + BWI76_RS11060 0.68 +0.8
Or see this region's nucleotide sequence