Experiment: KLEB16 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02940 and BWI76_RS02945 are separated by 260 nucleotides BWI76_RS02945 and BWI76_RS02950 are separated by 79 nucleotides
BWI76_RS02940: BWI76_RS02940 - RpiR family transcriptional regulator, at 601,371 to 602,114
_RS02940
BWI76_RS02945: BWI76_RS02945 - PTS sugar transporter subunit IIB, at 602,375 to 602,683
_RS02945
BWI76_RS02950: BWI76_RS02950 - 6-phospho-beta-glucosidase, at 602,763 to 604,070
_RS02950
Position (kb) 602
603 Strain fitness (log2 ratio) -2
-1
0
1 at 601.395 kb on - strand at 601.442 kb on - strand at 601.446 kb on - strand, within BWI76_RS02940 at 601.535 kb on + strand, within BWI76_RS02940 at 601.699 kb on + strand, within BWI76_RS02940 at 601.887 kb on - strand, within BWI76_RS02940 at 602.079 kb on + strand at 602.344 kb on - strand at 602.382 kb on + strand at 602.390 kb on - strand at 602.575 kb on + strand at 602.669 kb on + strand at 602.731 kb on + strand at 602.811 kb on - strand at 602.811 kb on - strand at 602.827 kb on + strand at 602.827 kb on + strand at 602.835 kb on - strand at 602.927 kb on + strand, within BWI76_RS02950 at 603.340 kb on + strand, within BWI76_RS02950 at 603.402 kb on + strand, within BWI76_RS02950 at 603.673 kb on + strand, within BWI76_RS02950
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 1 remove 601,395 - +0.5 601,442 - +0.7 601,446 - BWI76_RS02940 0.10 -1.2 601,535 + BWI76_RS02940 0.22 +0.1 601,699 + BWI76_RS02940 0.44 -1.9 601,887 - BWI76_RS02940 0.69 +0.1 602,079 + +0.5 602,344 - +0.6 602,382 + +0.4 602,390 - -1.8 602,575 + -0.4 602,669 + -1.3 602,731 + +0.3 602,811 - +0.9 602,811 - -0.8 602,827 + +1.0 602,827 + +0.2 602,835 - -0.0 602,927 + BWI76_RS02950 0.13 +0.2 603,340 + BWI76_RS02950 0.44 -0.5 603,402 + BWI76_RS02950 0.49 -0.1 603,673 + BWI76_RS02950 0.70 -2.0
Or see this region's nucleotide sequence