Experiment: KLEB16 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00645 and BWI76_RS00650 are separated by 148 nucleotides BWI76_RS00650 and BWI76_RS00655 overlap by 4 nucleotides
BWI76_RS00645: BWI76_RS00645 - lactaldehyde reductase, at 130,999 to 132,147
_RS00645
BWI76_RS00650: BWI76_RS00650 - rhamnose ABC transporter permease component, at 132,296 to 133,300
_RS00650
BWI76_RS00655: BWI76_RS00655 - inner-membrane translocator, at 133,297 to 134,301
_RS00655
Position (kb) 132
133
134 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 131.386 kb on + strand, within BWI76_RS00645 at 131.386 kb on + strand, within BWI76_RS00645 at 131.394 kb on - strand, within BWI76_RS00645 at 131.512 kb on - strand, within BWI76_RS00645 at 131.555 kb on - strand, within BWI76_RS00645 at 131.563 kb on - strand, within BWI76_RS00645 at 131.674 kb on + strand, within BWI76_RS00645 at 131.792 kb on - strand, within BWI76_RS00645 at 131.945 kb on - strand, within BWI76_RS00645 at 132.025 kb on - strand, within BWI76_RS00645 at 132.136 kb on + strand at 132.186 kb on + strand at 132.266 kb on - strand at 132.523 kb on + strand, within BWI76_RS00650 at 132.619 kb on + strand, within BWI76_RS00650 at 132.684 kb on + strand, within BWI76_RS00650 at 132.786 kb on - strand, within BWI76_RS00650 at 132.791 kb on - strand, within BWI76_RS00650 at 132.832 kb on + strand, within BWI76_RS00650 at 132.897 kb on + strand, within BWI76_RS00650 at 132.907 kb on + strand, within BWI76_RS00650 at 132.947 kb on + strand, within BWI76_RS00650 at 133.029 kb on - strand, within BWI76_RS00650 at 133.229 kb on - strand at 133.229 kb on - strand at 133.284 kb on - strand at 133.344 kb on + strand at 133.344 kb on + strand at 133.472 kb on - strand at 133.729 kb on + strand, within BWI76_RS00655 at 133.737 kb on - strand, within BWI76_RS00655 at 133.761 kb on + strand, within BWI76_RS00655 at 133.771 kb on - strand, within BWI76_RS00655 at 133.796 kb on + strand, within BWI76_RS00655 at 133.866 kb on - strand, within BWI76_RS00655 at 133.935 kb on - strand, within BWI76_RS00655 at 133.974 kb on - strand, within BWI76_RS00655 at 134.062 kb on - strand, within BWI76_RS00655 at 134.085 kb on + strand, within BWI76_RS00655 at 134.125 kb on - strand, within BWI76_RS00655 at 134.187 kb on + strand, within BWI76_RS00655 at 134.209 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 1 remove 131,386 + BWI76_RS00645 0.34 +0.5 131,386 + BWI76_RS00645 0.34 +0.4 131,394 - BWI76_RS00645 0.34 -0.6 131,512 - BWI76_RS00645 0.45 +0.3 131,555 - BWI76_RS00645 0.48 -0.3 131,563 - BWI76_RS00645 0.49 -0.3 131,674 + BWI76_RS00645 0.59 -0.1 131,792 - BWI76_RS00645 0.69 -0.4 131,945 - BWI76_RS00645 0.82 +0.1 132,025 - BWI76_RS00645 0.89 -1.1 132,136 + -0.2 132,186 + -3.3 132,266 - -0.6 132,523 + BWI76_RS00650 0.23 -0.3 132,619 + BWI76_RS00650 0.32 -1.3 132,684 + BWI76_RS00650 0.39 -1.3 132,786 - BWI76_RS00650 0.49 +0.4 132,791 - BWI76_RS00650 0.49 -0.8 132,832 + BWI76_RS00650 0.53 -1.8 132,897 + BWI76_RS00650 0.60 -0.2 132,907 + BWI76_RS00650 0.61 -2.4 132,947 + BWI76_RS00650 0.65 -1.3 133,029 - BWI76_RS00650 0.73 -0.4 133,229 - -0.6 133,229 - -0.5 133,284 - -0.1 133,344 + -0.3 133,344 + -0.6 133,472 - +1.9 133,729 + BWI76_RS00655 0.43 -0.8 133,737 - BWI76_RS00655 0.44 -0.2 133,761 + BWI76_RS00655 0.46 +0.3 133,771 - BWI76_RS00655 0.47 -0.7 133,796 + BWI76_RS00655 0.50 -0.1 133,866 - BWI76_RS00655 0.57 -2.3 133,935 - BWI76_RS00655 0.63 +0.6 133,974 - BWI76_RS00655 0.67 -1.2 134,062 - BWI76_RS00655 0.76 +0.6 134,085 + BWI76_RS00655 0.78 -0.6 134,125 - BWI76_RS00655 0.82 -2.4 134,187 + BWI76_RS00655 0.89 -0.9 134,209 + +0.3
Or see this region's nucleotide sequence