Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS20665

Experiment: KLEB16 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS20650 and BWI76_RS20655 are separated by 42 nucleotidesBWI76_RS20655 and BWI76_RS20660 are separated by 42 nucleotidesBWI76_RS20660 and BWI76_RS20665 overlap by 4 nucleotidesBWI76_RS20665 and BWI76_RS20670 are separated by 82 nucleotides BWI76_RS20650: BWI76_RS20650 - phosphoenolpyruvate--protein phosphotransferase, at 4,320,863 to 4,322,590 _RS20650 BWI76_RS20655: BWI76_RS20655 - glucose-specific phosphotransferase enzyme IIA component, at 4,322,633 to 4,323,142 _RS20655 BWI76_RS20660: BWI76_RS20660 - hypothetical protein, at 4,323,185 to 4,323,424 _RS20660 BWI76_RS20665: BWI76_RS20665 - bifunctional pyridoxal kinase/hydroxymethylpyrimidine kinase, at 4,323,421 to 4,324,287 _RS20665 BWI76_RS20670: BWI76_RS20670 - transcriptional regulator PtsJ, at 4,324,370 to 4,325,668 _RS20670 Position (kb) 4323 4324 4325Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4322.527 kb on + strandat 4323.795 kb on + strand, within BWI76_RS20665at 4323.959 kb on + strand, within BWI76_RS20665at 4323.967 kb on - strand, within BWI76_RS20665at 4324.019 kb on + strand, within BWI76_RS20665at 4324.114 kb on - strand, within BWI76_RS20665at 4324.152 kb on + strand, within BWI76_RS20665at 4324.189 kb on + strand, within BWI76_RS20665at 4324.220 kb on + strandat 4324.515 kb on + strand, within BWI76_RS20670at 4324.533 kb on + strand, within BWI76_RS20670at 4325.054 kb on - strand, within BWI76_RS20670at 4325.140 kb on + strand, within BWI76_RS20670

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB16 MOI 10
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4,322,527 + -1.4
4,323,795 + BWI76_RS20665 0.43 -2.1
4,323,959 + BWI76_RS20665 0.62 +0.2
4,323,967 - BWI76_RS20665 0.63 +1.8
4,324,019 + BWI76_RS20665 0.69 -0.8
4,324,114 - BWI76_RS20665 0.80 -3.7
4,324,152 + BWI76_RS20665 0.84 -1.0
4,324,189 + BWI76_RS20665 0.89 -0.5
4,324,220 + +0.8
4,324,515 + BWI76_RS20670 0.11 -0.1
4,324,533 + BWI76_RS20670 0.13 -0.2
4,325,054 - BWI76_RS20670 0.53 +0.0
4,325,140 + BWI76_RS20670 0.59 +0.1

Or see this region's nucleotide sequence