Experiment: KLEB16 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02845 and BWI76_RS02850 are separated by 94 nucleotides BWI76_RS02850 and BWI76_RS02855 are separated by 36 nucleotides BWI76_RS02855 and BWI76_RS02860 are separated by 419 nucleotides
BWI76_RS02845: BWI76_RS02845 - hypothetical protein, at 583,181 to 583,732
_RS02845
BWI76_RS02850: BWI76_RS02850 - metalloprotease PmbA, at 583,827 to 585,179
_RS02850
BWI76_RS02855: BWI76_RS02855 - cytochrome B562, at 585,216 to 585,602
_RS02855
BWI76_RS02860: BWI76_RS02860 - glycine dehydrogenase, at 586,022 to 586,360
_RS02860
Position (kb) 583
584
585
586 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 582.963 kb on + strand at 583.801 kb on + strand at 583.866 kb on - strand at 583.876 kb on + strand at 583.886 kb on - strand at 584.057 kb on - strand, within BWI76_RS02850 at 584.202 kb on - strand, within BWI76_RS02850 at 584.204 kb on + strand, within BWI76_RS02850 at 584.441 kb on + strand, within BWI76_RS02850 at 584.449 kb on - strand, within BWI76_RS02850 at 584.509 kb on + strand, within BWI76_RS02850 at 584.516 kb on - strand, within BWI76_RS02850 at 584.800 kb on + strand, within BWI76_RS02850 at 584.808 kb on - strand, within BWI76_RS02850 at 585.139 kb on + strand at 585.305 kb on - strand, within BWI76_RS02855 at 585.328 kb on - strand, within BWI76_RS02855 at 585.480 kb on - strand, within BWI76_RS02855 at 585.568 kb on - strand at 585.981 kb on + strand at 585.981 kb on + strand at 586.011 kb on - strand at 586.055 kb on - strand at 586.112 kb on + strand, within BWI76_RS02860
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 10 remove 582,963 + -0.3 583,801 + -0.4 583,866 - +2.7 583,876 + -0.2 583,886 - -0.4 584,057 - BWI76_RS02850 0.17 -0.4 584,202 - BWI76_RS02850 0.28 +1.6 584,204 + BWI76_RS02850 0.28 +0.5 584,441 + BWI76_RS02850 0.45 +1.0 584,449 - BWI76_RS02850 0.46 +0.7 584,509 + BWI76_RS02850 0.50 +2.7 584,516 - BWI76_RS02850 0.51 -4.0 584,800 + BWI76_RS02850 0.72 -0.4 584,808 - BWI76_RS02850 0.73 -0.1 585,139 + +0.5 585,305 - BWI76_RS02855 0.23 -0.6 585,328 - BWI76_RS02855 0.29 -0.8 585,480 - BWI76_RS02855 0.68 -1.7 585,568 - +0.0 585,981 + -0.2 585,981 + -0.3 586,011 - +0.2 586,055 - -0.8 586,112 + BWI76_RS02860 0.27 +1.2
Or see this region's nucleotide sequence