Experiment: KLEB16 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02720 and BWI76_RS02725 are separated by 84 nucleotides BWI76_RS02725 and BWI76_RS02730 are separated by 52 nucleotides BWI76_RS02730 and BWI76_RS02735 are separated by 191 nucleotides BWI76_RS02735 and BWI76_RS02740 overlap by 11 nucleotides
BWI76_RS02720: BWI76_RS02720 - NAD(P)-dependent oxidoreductase, at 551,086 to 551,934
_RS02720
BWI76_RS02725: BWI76_RS02725 - HxlR family transcriptional regulator, at 552,019 to 552,402
_RS02725
BWI76_RS02730: BWI76_RS02730 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 552,455 to 554,398
_RS02730
BWI76_RS02735: BWI76_RS02735 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 554,590 to 555,333
_RS02735
BWI76_RS02740: BWI76_RS02740 - putative transcriptional regulator, at 555,323 to 555,880
_RS02740
Position (kb) 552
553
554
555 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 551.543 kb on - strand, within BWI76_RS02720 at 551.604 kb on - strand, within BWI76_RS02720 at 551.666 kb on + strand, within BWI76_RS02720 at 551.854 kb on - strand at 551.901 kb on - strand at 551.937 kb on + strand at 552.264 kb on - strand, within BWI76_RS02725 at 552.409 kb on + strand at 552.437 kb on - strand at 552.688 kb on + strand, within BWI76_RS02730 at 553.210 kb on + strand, within BWI76_RS02730 at 553.225 kb on + strand, within BWI76_RS02730 at 553.524 kb on + strand, within BWI76_RS02730 at 553.902 kb on + strand, within BWI76_RS02730 at 553.991 kb on + strand, within BWI76_RS02730 at 554.159 kb on + strand, within BWI76_RS02730 at 554.203 kb on - strand, within BWI76_RS02730 at 554.227 kb on + strand at 554.227 kb on + strand at 554.267 kb on + strand at 554.380 kb on - strand at 554.390 kb on - strand at 554.431 kb on + strand at 554.567 kb on + strand at 554.567 kb on + strand at 554.736 kb on - strand, within BWI76_RS02735 at 554.803 kb on - strand, within BWI76_RS02735 at 554.963 kb on - strand, within BWI76_RS02735 at 555.079 kb on - strand, within BWI76_RS02735 at 555.173 kb on - strand, within BWI76_RS02735 at 555.207 kb on - strand, within BWI76_RS02735
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 10 remove 551,543 - BWI76_RS02720 0.54 -0.4 551,604 - BWI76_RS02720 0.61 +0.5 551,666 + BWI76_RS02720 0.68 -1.2 551,854 - -0.1 551,901 - +2.3 551,937 + +1.3 552,264 - BWI76_RS02725 0.64 -0.4 552,409 + +1.3 552,437 - +0.1 552,688 + BWI76_RS02730 0.12 -2.6 553,210 + BWI76_RS02730 0.39 -2.2 553,225 + BWI76_RS02730 0.40 -0.2 553,524 + BWI76_RS02730 0.55 -0.0 553,902 + BWI76_RS02730 0.74 -0.8 553,991 + BWI76_RS02730 0.79 -0.9 554,159 + BWI76_RS02730 0.88 -1.6 554,203 - BWI76_RS02730 0.90 -1.1 554,227 + +0.5 554,227 + +0.4 554,267 + -0.3 554,380 - +1.1 554,390 - +1.8 554,431 + -0.9 554,567 + -0.3 554,567 + -0.7 554,736 - BWI76_RS02735 0.20 -3.3 554,803 - BWI76_RS02735 0.29 -0.4 554,963 - BWI76_RS02735 0.50 -1.0 555,079 - BWI76_RS02735 0.66 -0.7 555,173 - BWI76_RS02735 0.78 +1.1 555,207 - BWI76_RS02735 0.83 +3.0
Or see this region's nucleotide sequence