Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS02730

Experiment: KLEB16 MOI 10

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS02720 and BWI76_RS02725 are separated by 84 nucleotidesBWI76_RS02725 and BWI76_RS02730 are separated by 52 nucleotidesBWI76_RS02730 and BWI76_RS02735 are separated by 191 nucleotidesBWI76_RS02735 and BWI76_RS02740 overlap by 11 nucleotides BWI76_RS02720: BWI76_RS02720 - NAD(P)-dependent oxidoreductase, at 551,086 to 551,934 _RS02720 BWI76_RS02725: BWI76_RS02725 - HxlR family transcriptional regulator, at 552,019 to 552,402 _RS02725 BWI76_RS02730: BWI76_RS02730 - 2',3'-cyclic-nucleotide 2'-phosphodiesterase, at 552,455 to 554,398 _RS02730 BWI76_RS02735: BWI76_RS02735 - 3'(2'),5'-bisphosphate nucleotidase CysQ, at 554,590 to 555,333 _RS02735 BWI76_RS02740: BWI76_RS02740 - putative transcriptional regulator, at 555,323 to 555,880 _RS02740 Position (kb) 552 553 554 555Strain fitness (log2 ratio) -3 -2 -1 0 1 2 3at 551.543 kb on - strand, within BWI76_RS02720at 551.604 kb on - strand, within BWI76_RS02720at 551.666 kb on + strand, within BWI76_RS02720at 551.854 kb on - strandat 551.901 kb on - strandat 551.937 kb on + strandat 552.264 kb on - strand, within BWI76_RS02725at 552.409 kb on + strandat 552.437 kb on - strandat 552.688 kb on + strand, within BWI76_RS02730at 553.210 kb on + strand, within BWI76_RS02730at 553.225 kb on + strand, within BWI76_RS02730at 553.524 kb on + strand, within BWI76_RS02730at 553.902 kb on + strand, within BWI76_RS02730at 553.991 kb on + strand, within BWI76_RS02730at 554.159 kb on + strand, within BWI76_RS02730at 554.203 kb on - strand, within BWI76_RS02730at 554.227 kb on + strandat 554.227 kb on + strandat 554.267 kb on + strandat 554.380 kb on - strandat 554.390 kb on - strandat 554.431 kb on + strandat 554.567 kb on + strandat 554.567 kb on + strandat 554.736 kb on - strand, within BWI76_RS02735at 554.803 kb on - strand, within BWI76_RS02735at 554.963 kb on - strand, within BWI76_RS02735at 555.079 kb on - strand, within BWI76_RS02735at 555.173 kb on - strand, within BWI76_RS02735at 555.207 kb on - strand, within BWI76_RS02735

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB16 MOI 10
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551,543 - BWI76_RS02720 0.54 -0.4
551,604 - BWI76_RS02720 0.61 +0.5
551,666 + BWI76_RS02720 0.68 -1.2
551,854 - -0.1
551,901 - +2.3
551,937 + +1.3
552,264 - BWI76_RS02725 0.64 -0.4
552,409 + +1.3
552,437 - +0.1
552,688 + BWI76_RS02730 0.12 -2.6
553,210 + BWI76_RS02730 0.39 -2.2
553,225 + BWI76_RS02730 0.40 -0.2
553,524 + BWI76_RS02730 0.55 -0.0
553,902 + BWI76_RS02730 0.74 -0.8
553,991 + BWI76_RS02730 0.79 -0.9
554,159 + BWI76_RS02730 0.88 -1.6
554,203 - BWI76_RS02730 0.90 -1.1
554,227 + +0.5
554,227 + +0.4
554,267 + -0.3
554,380 - +1.1
554,390 - +1.8
554,431 + -0.9
554,567 + -0.3
554,567 + -0.7
554,736 - BWI76_RS02735 0.20 -3.3
554,803 - BWI76_RS02735 0.29 -0.4
554,963 - BWI76_RS02735 0.50 -1.0
555,079 - BWI76_RS02735 0.66 -0.7
555,173 - BWI76_RS02735 0.78 +1.1
555,207 - BWI76_RS02735 0.83 +3.0

Or see this region's nucleotide sequence