Experiment: KLEB16 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS02115 and BWI76_RS02120 are separated by 198 nucleotides BWI76_RS02120 and BWI76_RS02125 overlap by 4 nucleotides
BWI76_RS02115: BWI76_RS02115 - formate dehydrogenase subunit alpha, at 436,441 to 438,588
_RS02115
BWI76_RS02120: BWI76_RS02120 - multidrug resistance transporter, at 438,787 to 440,250
_RS02120
BWI76_RS02125: BWI76_RS02125 - multidrug efflux system protein MdtO, at 440,247 to 442,289
_RS02125
Position (kb) 438
439
440
441 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 437.851 kb on - strand, within BWI76_RS02115 at 437.911 kb on - strand, within BWI76_RS02115 at 437.911 kb on - strand, within BWI76_RS02115 at 438.064 kb on - strand, within BWI76_RS02115 at 438.438 kb on + strand at 438.849 kb on + strand at 438.986 kb on + strand, within BWI76_RS02120 at 439.067 kb on + strand, within BWI76_RS02120 at 439.091 kb on - strand, within BWI76_RS02120 at 439.113 kb on - strand, within BWI76_RS02120 at 439.132 kb on - strand, within BWI76_RS02120 at 439.168 kb on + strand, within BWI76_RS02120 at 439.249 kb on - strand, within BWI76_RS02120 at 439.309 kb on + strand, within BWI76_RS02120 at 439.388 kb on - strand, within BWI76_RS02120 at 439.582 kb on - strand, within BWI76_RS02120 at 439.582 kb on - strand, within BWI76_RS02120 at 439.582 kb on - strand, within BWI76_RS02120 at 439.603 kb on + strand, within BWI76_RS02120 at 439.632 kb on + strand, within BWI76_RS02120 at 439.803 kb on + strand, within BWI76_RS02120 at 439.803 kb on + strand, within BWI76_RS02120 at 439.803 kb on + strand, within BWI76_RS02120 at 439.818 kb on + strand, within BWI76_RS02120 at 439.828 kb on - strand, within BWI76_RS02120 at 439.875 kb on + strand, within BWI76_RS02120 at 439.957 kb on - strand, within BWI76_RS02120 at 440.057 kb on + strand, within BWI76_RS02120 at 440.094 kb on - strand, within BWI76_RS02120 at 440.138 kb on + strand at 440.141 kb on - strand at 440.209 kb on - strand at 440.245 kb on + strand at 440.246 kb on - strand at 440.428 kb on - strand at 440.505 kb on - strand, within BWI76_RS02125 at 440.910 kb on + strand, within BWI76_RS02125 at 440.910 kb on + strand, within BWI76_RS02125 at 441.021 kb on - strand, within BWI76_RS02125 at 441.152 kb on - strand, within BWI76_RS02125 at 441.156 kb on + strand, within BWI76_RS02125 at 441.164 kb on - strand, within BWI76_RS02125
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 10 remove 437,851 - BWI76_RS02115 0.66 -0.7 437,911 - BWI76_RS02115 0.68 -2.1 437,911 - BWI76_RS02115 0.68 -0.8 438,064 - BWI76_RS02115 0.76 +0.6 438,438 + -0.3 438,849 + -1.3 438,986 + BWI76_RS02120 0.14 -1.4 439,067 + BWI76_RS02120 0.19 -0.4 439,091 - BWI76_RS02120 0.21 +0.4 439,113 - BWI76_RS02120 0.22 -0.7 439,132 - BWI76_RS02120 0.24 +0.5 439,168 + BWI76_RS02120 0.26 +0.0 439,249 - BWI76_RS02120 0.32 -2.3 439,309 + BWI76_RS02120 0.36 -0.2 439,388 - BWI76_RS02120 0.41 -3.4 439,582 - BWI76_RS02120 0.54 +0.5 439,582 - BWI76_RS02120 0.54 -0.9 439,582 - BWI76_RS02120 0.54 -3.5 439,603 + BWI76_RS02120 0.56 -0.1 439,632 + BWI76_RS02120 0.58 -0.7 439,803 + BWI76_RS02120 0.69 -1.6 439,803 + BWI76_RS02120 0.69 -0.8 439,803 + BWI76_RS02120 0.69 +0.1 439,818 + BWI76_RS02120 0.70 -0.9 439,828 - BWI76_RS02120 0.71 -0.5 439,875 + BWI76_RS02120 0.74 +0.3 439,957 - BWI76_RS02120 0.80 -1.2 440,057 + BWI76_RS02120 0.87 -1.2 440,094 - BWI76_RS02120 0.89 -0.1 440,138 + +0.1 440,141 - -0.7 440,209 - -0.9 440,245 + -0.2 440,246 - +0.5 440,428 - +0.3 440,505 - BWI76_RS02125 0.13 -0.2 440,910 + BWI76_RS02125 0.32 -0.2 440,910 + BWI76_RS02125 0.32 +0.7 441,021 - BWI76_RS02125 0.38 +0.0 441,152 - BWI76_RS02125 0.44 +1.2 441,156 + BWI76_RS02125 0.44 +1.2 441,164 - BWI76_RS02125 0.45 -1.2
Or see this region's nucleotide sequence