Experiment: KLEB16 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS01250 and BWI76_RS01255 are separated by 246 nucleotides BWI76_RS01255 and BWI76_RS01260 are separated by 109 nucleotides BWI76_RS01260 and BWI76_RS01265 are separated by 43 nucleotides BWI76_RS01265 and BWI76_RS01270 are separated by 88 nucleotides
BWI76_RS01250: BWI76_RS01250 - threonine export protein RhtC, at 256,483 to 257,106
_RS01250
BWI76_RS01255: BWI76_RS01255 - homoserine/homoserine lactone efflux protein, at 257,353 to 257,973
_RS01255
BWI76_RS01260: BWI76_RS01260 - lysophospholipase L2, at 258,083 to 259,075
_RS01260
BWI76_RS01265: BWI76_RS01265 - sugar/pyridoxal phosphate phosphatase YigL, at 259,119 to 259,919
_RS01265
BWI76_RS01270: BWI76_RS01270 - hypothetical protein, at 260,008 to 260,907
_RS01270
Position (kb) 258
259
260 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 257.129 kb on + strand at 257.137 kb on - strand at 257.179 kb on + strand at 257.379 kb on - strand at 257.379 kb on - strand at 257.464 kb on - strand, within BWI76_RS01255 at 257.533 kb on + strand, within BWI76_RS01255 at 257.543 kb on + strand, within BWI76_RS01255 at 257.551 kb on - strand, within BWI76_RS01255 at 257.687 kb on - strand, within BWI76_RS01255 at 257.689 kb on + strand, within BWI76_RS01255 at 257.696 kb on + strand, within BWI76_RS01255 at 257.761 kb on + strand, within BWI76_RS01255 at 257.769 kb on - strand, within BWI76_RS01255 at 257.811 kb on + strand, within BWI76_RS01255 at 257.853 kb on - strand at 258.014 kb on - strand at 258.080 kb on - strand at 258.332 kb on - strand, within BWI76_RS01260 at 258.485 kb on + strand, within BWI76_RS01260 at 258.620 kb on + strand, within BWI76_RS01260 at 258.620 kb on + strand, within BWI76_RS01260 at 258.628 kb on - strand, within BWI76_RS01260 at 258.689 kb on + strand, within BWI76_RS01260 at 258.907 kb on - strand, within BWI76_RS01260 at 258.955 kb on - strand, within BWI76_RS01260 at 258.963 kb on + strand, within BWI76_RS01260 at 259.069 kb on + strand at 259.099 kb on + strand at 259.099 kb on + strand at 259.186 kb on + strand at 259.508 kb on - strand, within BWI76_RS01265 at 259.635 kb on + strand, within BWI76_RS01265 at 259.660 kb on + strand, within BWI76_RS01265 at 259.701 kb on - strand, within BWI76_RS01265 at 259.750 kb on - strand, within BWI76_RS01265 at 259.760 kb on + strand, within BWI76_RS01265 at 259.769 kb on - strand, within BWI76_RS01265 at 259.806 kb on + strand, within BWI76_RS01265 at 259.846 kb on - strand at 260.025 kb on - strand at 260.056 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB16 MOI 10 remove 257,129 + -2.6 257,137 - +0.7 257,179 + +1.4 257,379 - -3.1 257,379 - +0.3 257,464 - BWI76_RS01255 0.18 +1.3 257,533 + BWI76_RS01255 0.29 +0.1 257,543 + BWI76_RS01255 0.31 +0.4 257,551 - BWI76_RS01255 0.32 -0.6 257,687 - BWI76_RS01255 0.54 +0.3 257,689 + BWI76_RS01255 0.54 +1.6 257,696 + BWI76_RS01255 0.55 -0.3 257,761 + BWI76_RS01255 0.66 +1.5 257,769 - BWI76_RS01255 0.67 +0.7 257,811 + BWI76_RS01255 0.74 +0.3 257,853 - -1.0 258,014 - -2.0 258,080 - -1.5 258,332 - BWI76_RS01260 0.25 +0.9 258,485 + BWI76_RS01260 0.40 +0.7 258,620 + BWI76_RS01260 0.54 +0.3 258,620 + BWI76_RS01260 0.54 -1.6 258,628 - BWI76_RS01260 0.55 +0.9 258,689 + BWI76_RS01260 0.61 +0.3 258,907 - BWI76_RS01260 0.83 +1.1 258,955 - BWI76_RS01260 0.88 -0.1 258,963 + BWI76_RS01260 0.89 +0.3 259,069 + +1.0 259,099 + -0.9 259,099 + +0.3 259,186 + +0.5 259,508 - BWI76_RS01265 0.49 +0.0 259,635 + BWI76_RS01265 0.64 -0.0 259,660 + BWI76_RS01265 0.68 +0.5 259,701 - BWI76_RS01265 0.73 +0.7 259,750 - BWI76_RS01265 0.79 +0.4 259,760 + BWI76_RS01265 0.80 -0.1 259,769 - BWI76_RS01265 0.81 +1.6 259,806 + BWI76_RS01265 0.86 +0.6 259,846 - +0.8 260,025 - +0.2 260,056 - -2.5
Or see this region's nucleotide sequence