Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20520 and BWI76_RS20525 are separated by 176 nucleotides BWI76_RS20525 and BWI76_RS20530 are separated by 130 nucleotides BWI76_RS20530 and BWI76_RS20535 are separated by 48 nucleotides
BWI76_RS20520: BWI76_RS20520 - indolepyruvate decarboxylase, at 4,297,529 to 4,299,190
_RS20520
BWI76_RS20525: BWI76_RS20525 - glyceraldehyde 3-phosphate reductase, at 4,299,367 to 4,300,359
_RS20525
BWI76_RS20530: BWI76_RS20530 - hypothetical protein, at 4,300,490 to 4,300,849
_RS20530
BWI76_RS20535: BWI76_RS20535 - manganese transporter MntH, at 4,300,898 to 4,302,133
_RS20535
Position (kb)
4299
4300
4301 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4298.629 kb on + strand, within BWI76_RS20520 at 4298.672 kb on - strand, within BWI76_RS20520 at 4298.885 kb on - strand, within BWI76_RS20520 at 4298.905 kb on - strand, within BWI76_RS20520 at 4298.914 kb on - strand, within BWI76_RS20520 at 4299.045 kb on - strand at 4299.245 kb on + strand at 4299.253 kb on - strand at 4299.253 kb on - strand at 4299.253 kb on - strand at 4299.386 kb on - strand at 4299.756 kb on - strand, within BWI76_RS20525 at 4299.825 kb on + strand, within BWI76_RS20525 at 4299.979 kb on - strand, within BWI76_RS20525 at 4300.019 kb on + strand, within BWI76_RS20525 at 4300.207 kb on + strand, within BWI76_RS20525 at 4300.215 kb on - strand, within BWI76_RS20525 at 4300.215 kb on - strand, within BWI76_RS20525 at 4300.241 kb on + strand, within BWI76_RS20525 at 4300.275 kb on + strand at 4300.417 kb on - strand at 4300.434 kb on + strand at 4300.447 kb on + strand at 4300.488 kb on - strand at 4300.563 kb on + strand, within BWI76_RS20530 at 4300.704 kb on - strand, within BWI76_RS20530 at 4300.726 kb on + strand, within BWI76_RS20530
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 4,298,629 + BWI76_RS20520 0.66 +3.2 4,298,672 - BWI76_RS20520 0.69 +1.3 4,298,885 - BWI76_RS20520 0.82 +0.7 4,298,905 - BWI76_RS20520 0.83 +2.0 4,298,914 - BWI76_RS20520 0.83 -0.2 4,299,045 - +0.5 4,299,245 + -0.9 4,299,253 - -0.2 4,299,253 - -0.6 4,299,253 - -1.2 4,299,386 - -0.5 4,299,756 - BWI76_RS20525 0.39 +0.7 4,299,825 + BWI76_RS20525 0.46 +0.1 4,299,979 - BWI76_RS20525 0.62 +0.2 4,300,019 + BWI76_RS20525 0.66 +0.2 4,300,207 + BWI76_RS20525 0.85 -0.6 4,300,215 - BWI76_RS20525 0.85 -0.4 4,300,215 - BWI76_RS20525 0.85 +0.2 4,300,241 + BWI76_RS20525 0.88 +0.1 4,300,275 + -0.6 4,300,417 - -1.8 4,300,434 + +0.1 4,300,447 + -0.4 4,300,488 - +0.9 4,300,563 + BWI76_RS20530 0.20 +0.3 4,300,704 - BWI76_RS20530 0.59 +0.0 4,300,726 + BWI76_RS20530 0.66 +0.8
Or see this region's nucleotide sequence