Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10245 and BWI76_RS10250 are separated by 68 nucleotides BWI76_RS10250 and BWI76_RS10255 overlap by 17 nucleotides BWI76_RS10255 and BWI76_RS10260 are separated by 10 nucleotides
BWI76_RS10245: BWI76_RS10245 - aspartate aminotransferase, at 2,169,600 to 2,170,808
_RS10245
BWI76_RS10250: BWI76_RS10250 - glutamine amidotransferase, at 2,170,877 to 2,171,974
_RS10250
BWI76_RS10255: BWI76_RS10255 - dihydrodipicolinate synthase family protein, at 2,171,958 to 2,172,881
_RS10255
BWI76_RS10260: BWI76_RS10260 - creatinase, at 2,172,892 to 2,174,061
_RS10260
Position (kb)
2170
2171
2172 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2170.526 kb on + strand, within BWI76_RS10245 at 2170.729 kb on + strand at 2171.157 kb on - strand, within BWI76_RS10250 at 2171.184 kb on - strand, within BWI76_RS10250 at 2171.448 kb on + strand, within BWI76_RS10250 at 2171.456 kb on - strand, within BWI76_RS10250 at 2171.456 kb on - strand, within BWI76_RS10250 at 2171.552 kb on + strand, within BWI76_RS10250 at 2171.900 kb on - strand at 2172.023 kb on - strand at 2172.023 kb on - strand at 2172.023 kb on - strand at 2172.668 kb on - strand, within BWI76_RS10255 at 2172.750 kb on + strand, within BWI76_RS10255 at 2172.750 kb on + strand, within BWI76_RS10255
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 2,170,526 + BWI76_RS10245 0.77 +0.6 2,170,729 + +0.2 2,171,157 - BWI76_RS10250 0.26 +0.5 2,171,184 - BWI76_RS10250 0.28 +0.5 2,171,448 + BWI76_RS10250 0.52 +0.7 2,171,456 - BWI76_RS10250 0.53 +0.1 2,171,456 - BWI76_RS10250 0.53 +0.7 2,171,552 + BWI76_RS10250 0.61 -0.0 2,171,900 - -3.2 2,172,023 - +0.2 2,172,023 - +1.3 2,172,023 - +0.1 2,172,668 - BWI76_RS10255 0.77 +1.6 2,172,750 + BWI76_RS10255 0.86 +0.3 2,172,750 + BWI76_RS10255 0.86 -0.6
Or see this region's nucleotide sequence