Experiment: KLEB15 MOI 0.1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS09850 and BWI76_RS09855 overlap by 64 nucleotides BWI76_RS09855 and BWI76_RS09860 are separated by 149 nucleotides BWI76_RS09860 and BWI76_RS09865 are separated by 13 nucleotides BWI76_RS09865 and BWI76_RS09870 are separated by 60 nucleotides
BWI76_RS09850: BWI76_RS09850 - L,D-transpeptidase, at 2,069,449 to 2,071,242
_RS09850
BWI76_RS09855: BWI76_RS09855 - hypothetical protein, at 2,071,179 to 2,071,393
_RS09855
BWI76_RS09860: BWI76_RS09860 - hypothetical protein, at 2,071,543 to 2,072,094
_RS09860
BWI76_RS09865: BWI76_RS09865 - MBL fold hydrolase, at 2,072,108 to 2,072,755
_RS09865
BWI76_RS09870: BWI76_RS09870 - aromatic amino acid aminotransferase, at 2,072,816 to 2,074,006
_RS09870
Position (kb)
2072
2073 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2071.332 kb on + strand, within BWI76_RS09855 at 2071.433 kb on + strand at 2071.455 kb on + strand at 2071.455 kb on + strand at 2071.478 kb on + strand at 2071.818 kb on + strand, within BWI76_RS09860 at 2071.818 kb on + strand, within BWI76_RS09860 at 2071.826 kb on - strand, within BWI76_RS09860 at 2071.826 kb on - strand, within BWI76_RS09860 at 2072.025 kb on - strand, within BWI76_RS09860 at 2072.244 kb on + strand, within BWI76_RS09865 at 2072.262 kb on - strand, within BWI76_RS09865 at 2072.312 kb on + strand, within BWI76_RS09865 at 2072.317 kb on + strand, within BWI76_RS09865 at 2072.317 kb on + strand, within BWI76_RS09865 at 2072.317 kb on + strand, within BWI76_RS09865 at 2072.325 kb on - strand, within BWI76_RS09865 at 2073.464 kb on - strand, within BWI76_RS09870 at 2073.577 kb on + strand, within BWI76_RS09870 at 2073.605 kb on + strand, within BWI76_RS09870
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 0.1 remove 2,071,332 + BWI76_RS09855 0.71 +1.1 2,071,433 + -0.2 2,071,455 + -0.4 2,071,455 + -0.2 2,071,478 + +0.1 2,071,818 + BWI76_RS09860 0.50 +0.5 2,071,818 + BWI76_RS09860 0.50 +0.0 2,071,826 - BWI76_RS09860 0.51 +2.3 2,071,826 - BWI76_RS09860 0.51 -0.9 2,072,025 - BWI76_RS09860 0.87 +0.2 2,072,244 + BWI76_RS09865 0.21 -3.1 2,072,262 - BWI76_RS09865 0.24 +0.5 2,072,312 + BWI76_RS09865 0.31 +0.2 2,072,317 + BWI76_RS09865 0.32 -0.6 2,072,317 + BWI76_RS09865 0.32 -1.2 2,072,317 + BWI76_RS09865 0.32 -2.6 2,072,325 - BWI76_RS09865 0.33 -0.7 2,073,464 - BWI76_RS09870 0.54 -1.9 2,073,577 + BWI76_RS09870 0.64 -1.2 2,073,605 + BWI76_RS09870 0.66 -1.2
Or see this region's nucleotide sequence