Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27350 and BWI76_RS27355 are separated by 198 nucleotides BWI76_RS27355 and BWI76_RS27360 are separated by 20 nucleotides BWI76_RS27360 and BWI76_RS27365 are separated by 216 nucleotides BWI76_RS27365 and BWI76_RS27370 are separated by 175 nucleotides
BWI76_RS27350: BWI76_RS27350 - hypothetical protein, at 5,677,498 to 5,678,175
_RS27350
BWI76_RS27355: BWI76_RS27355 - 50S ribosomal protein L33, at 5,678,374 to 5,678,541
_RS27355
BWI76_RS27360: BWI76_RS27360 - 50S ribosomal protein L28, at 5,678,562 to 5,678,798
_RS27360
BWI76_RS27365: BWI76_RS27365 - hypothetical protein, at 5,679,015 to 5,679,680
_RS27365
BWI76_RS27370: BWI76_RS27370 - bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate synthase, at 5,679,856 to 5,681,067
_RS27370
Position (kb)
5679
5680 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 5678.120 kb on + strand at 5678.121 kb on - strand at 5678.130 kb on + strand at 5678.321 kb on - strand at 5679.146 kb on + strand, within BWI76_RS27365 at 5679.146 kb on + strand, within BWI76_RS27365 at 5679.188 kb on - strand, within BWI76_RS27365 at 5679.397 kb on + strand, within BWI76_RS27365 at 5679.447 kb on - strand, within BWI76_RS27365 at 5679.480 kb on - strand, within BWI76_RS27365 at 5679.605 kb on + strand, within BWI76_RS27365 at 5679.711 kb on - strand at 5679.755 kb on + strand at 5679.816 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 5,678,120 + +0.1 5,678,121 - -0.1 5,678,130 + -0.4 5,678,321 - -0.1 5,679,146 + BWI76_RS27365 0.20 -1.9 5,679,146 + BWI76_RS27365 0.20 +2.9 5,679,188 - BWI76_RS27365 0.26 -1.4 5,679,397 + BWI76_RS27365 0.57 +0.1 5,679,447 - BWI76_RS27365 0.65 -1.1 5,679,480 - BWI76_RS27365 0.70 -0.6 5,679,605 + BWI76_RS27365 0.89 +0.2 5,679,711 - -1.3 5,679,755 + -0.7 5,679,816 - -0.9
Or see this region's nucleotide sequence