Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS17670

Experiment: KLEB15 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS17660 and BWI76_RS17665 overlap by 16 nucleotidesBWI76_RS17665 and BWI76_RS17670 are separated by 18 nucleotidesBWI76_RS17670 and BWI76_RS17675 overlap by 4 nucleotidesBWI76_RS17675 and BWI76_RS17680 overlap by 7 nucleotides BWI76_RS17660: BWI76_RS17660 - AraC/XylS familytranscription regulator, at 3,668,737 to 3,669,504 _RS17660 BWI76_RS17665: BWI76_RS17665 - proteasome-type protease, at 3,669,489 to 3,670,241 _RS17665 BWI76_RS17670: BWI76_RS17670 - transglutaminase, at 3,670,260 to 3,671,075 _RS17670 BWI76_RS17675: BWI76_RS17675 - hypothetical protein, at 3,671,072 to 3,672,001 _RS17675 BWI76_RS17680: BWI76_RS17680 - hypothetical protein, at 3,671,995 to 3,673,434 _RS17680 Position (kb) 3670 3671 3672Strain fitness (log2 ratio) -1 0 1at 3669.751 kb on + strand, within BWI76_RS17665at 3669.899 kb on - strand, within BWI76_RS17665at 3670.295 kb on + strandat 3670.295 kb on + strandat 3670.366 kb on - strand, within BWI76_RS17670at 3670.908 kb on + strand, within BWI76_RS17670at 3670.950 kb on + strand, within BWI76_RS17670at 3671.105 kb on + strandat 3671.377 kb on - strand, within BWI76_RS17675at 3671.538 kb on - strand, within BWI76_RS17675at 3671.694 kb on - strand, within BWI76_RS17675at 3671.967 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 1
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3,669,751 + BWI76_RS17665 0.35 -1.3
3,669,899 - BWI76_RS17665 0.54 -0.3
3,670,295 + +0.3
3,670,295 + -0.0
3,670,366 - BWI76_RS17670 0.13 +0.9
3,670,908 + BWI76_RS17670 0.79 +0.4
3,670,950 + BWI76_RS17670 0.85 +1.5
3,671,105 + +0.4
3,671,377 - BWI76_RS17675 0.33 +0.1
3,671,538 - BWI76_RS17675 0.50 +0.3
3,671,694 - BWI76_RS17675 0.67 -0.8
3,671,967 + +0.4

Or see this region's nucleotide sequence