Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15965 and BWI76_RS15970 are separated by 419 nucleotides BWI76_RS15970 and BWI76_RS15975 are separated by 176 nucleotides BWI76_RS15975 and BWI76_RS15980 are separated by 149 nucleotides
BWI76_RS15965: BWI76_RS15965 - MFS transporter, at 3,311,408 to 3,312,658
_RS15965
BWI76_RS15970: BWI76_RS15970 - carboxypeptidase M32, at 3,313,078 to 3,314,565
_RS15970
BWI76_RS15975: BWI76_RS15975 - hypothetical protein, at 3,314,742 to 3,315,167
_RS15975
BWI76_RS15980: BWI76_RS15980 - lysine transporter LysE, at 3,315,317 to 3,315,579
_RS15980
BWI76_RS15985: BWI76_RS15985 - LysR family transcriptional regulator, at 3,315,560 to 3,315,784
_RS15985
Position (kb) 3313
3314
3315 Strain fitness (log2 ratio) -1
0
1 at 3312.323 kb on + strand at 3312.842 kb on - strand at 3313.022 kb on + strand at 3313.144 kb on - strand at 3313.493 kb on - strand, within BWI76_RS15970 at 3314.303 kb on - strand, within BWI76_RS15970 at 3314.645 kb on + strand at 3314.730 kb on + strand at 3314.782 kb on - strand at 3314.982 kb on + strand, within BWI76_RS15975 at 3315.261 kb on - strand at 3315.433 kb on - strand, within BWI76_RS15980
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 3,312,323 + +1.5 3,312,842 - -0.7 3,313,022 + +0.4 3,313,144 - +0.2 3,313,493 - BWI76_RS15970 0.28 +1.3 3,314,303 - BWI76_RS15970 0.82 +0.5 3,314,645 + +1.0 3,314,730 + +0.8 3,314,782 - +0.6 3,314,982 + BWI76_RS15975 0.56 +1.5 3,315,261 - +0.5 3,315,433 - BWI76_RS15980 0.44 +0.4
Or see this region's nucleotide sequence