Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS15850 and BWI76_RS15855 overlap by 4 nucleotides BWI76_RS15855 and BWI76_RS15860 overlap by 4 nucleotides BWI76_RS15860 and BWI76_RS15865 overlap by 4 nucleotides BWI76_RS15865 and BWI76_RS15870 overlap by 4 nucleotides BWI76_RS15870 and BWI76_RS15875 are separated by 95 nucleotides
BWI76_RS15850: BWI76_RS15850 - DUF1317 domain-containing protein, at 3,293,076 to 3,293,234
_RS15850
BWI76_RS15855: BWI76_RS15855 - hypothetical protein, at 3,293,231 to 3,293,458
_RS15855
BWI76_RS15860: BWI76_RS15860 - adenine-specific DNA methyltransferase, at 3,293,455 to 3,294,114
_RS15860
BWI76_RS15865: BWI76_RS15865 - hypothetical protein, at 3,294,111 to 3,294,332
_RS15865
BWI76_RS15870: BWI76_RS15870 - hypothetical protein, at 3,294,329 to 3,295,048
_RS15870
BWI76_RS15875: BWI76_RS15875 - hypothetical protein, at 3,295,144 to 3,295,383
_RS15875
Position (kb)
3294
3295 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 3293.200 kb on - strand, within BWI76_RS15850 at 3293.215 kb on + strand, within BWI76_RS15850 at 3293.538 kb on + strand, within BWI76_RS15860 at 3293.610 kb on + strand, within BWI76_RS15860 at 3293.677 kb on - strand, within BWI76_RS15860 at 3293.694 kb on - strand, within BWI76_RS15860 at 3293.736 kb on + strand, within BWI76_RS15860 at 3293.814 kb on - strand, within BWI76_RS15860 at 3293.968 kb on - strand, within BWI76_RS15860 at 3294.160 kb on + strand, within BWI76_RS15865 at 3294.202 kb on - strand, within BWI76_RS15865 at 3294.202 kb on - strand, within BWI76_RS15865 at 3294.223 kb on + strand, within BWI76_RS15865 at 3295.027 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 3,293,200 - BWI76_RS15850 0.78 -0.1 3,293,215 + BWI76_RS15850 0.87 -2.6 3,293,538 + BWI76_RS15860 0.13 +2.4 3,293,610 + BWI76_RS15860 0.23 +0.9 3,293,677 - BWI76_RS15860 0.34 -0.2 3,293,694 - BWI76_RS15860 0.36 -0.1 3,293,736 + BWI76_RS15860 0.43 +0.0 3,293,814 - BWI76_RS15860 0.54 +1.5 3,293,968 - BWI76_RS15860 0.78 -1.4 3,294,160 + BWI76_RS15865 0.22 -3.4 3,294,202 - BWI76_RS15865 0.41 -0.7 3,294,202 - BWI76_RS15865 0.41 -2.2 3,294,223 + BWI76_RS15865 0.50 +1.0 3,295,027 + +0.6
Or see this region's nucleotide sequence