Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13045 and BWI76_RS13050 are separated by 148 nucleotides BWI76_RS13050 and BWI76_RS13055 are separated by 128 nucleotides BWI76_RS13055 and BWI76_RS13060 are separated by 226 nucleotides BWI76_RS13060 and BWI76_RS13065 are separated by 120 nucleotides
BWI76_RS13045: BWI76_RS13045 - hypothetical protein, at 2,722,147 to 2,722,542
_RS13045
BWI76_RS13050: BWI76_RS13050 - transcriptional regulator, at 2,722,691 to 2,723,125
_RS13050
BWI76_RS13055: BWI76_RS13055 - NmrA family protein, at 2,723,254 to 2,724,165
_RS13055
BWI76_RS13060: BWI76_RS13060 - glutathione S-transferase, at 2,724,392 to 2,724,997
_RS13060
BWI76_RS13065: BWI76_RS13065 - LysR family transcriptional regulator, at 2,725,118 to 2,725,993
_RS13065
Position (kb)
2723
2724
2725 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2722.350 kb on - strand, within BWI76_RS13045 at 2722.350 kb on - strand, within BWI76_RS13045 at 2722.356 kb on + strand, within BWI76_RS13045 at 2722.393 kb on + strand, within BWI76_RS13045 at 2722.416 kb on - strand, within BWI76_RS13045 at 2722.472 kb on - strand, within BWI76_RS13045 at 2722.590 kb on + strand at 2722.672 kb on + strand at 2722.673 kb on + strand at 2722.701 kb on + strand at 2722.920 kb on - strand, within BWI76_RS13050 at 2723.196 kb on + strand at 2723.535 kb on - strand, within BWI76_RS13055 at 2723.740 kb on + strand, within BWI76_RS13055 at 2723.740 kb on + strand, within BWI76_RS13055 at 2723.809 kb on + strand, within BWI76_RS13055 at 2724.098 kb on - strand at 2724.139 kb on + strand at 2724.509 kb on + strand, within BWI76_RS13060 at 2724.509 kb on + strand, within BWI76_RS13060 at 2724.647 kb on - strand, within BWI76_RS13060 at 2724.649 kb on + strand, within BWI76_RS13060 at 2724.954 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 2,722,350 - BWI76_RS13045 0.51 +0.4 2,722,350 - BWI76_RS13045 0.51 -0.5 2,722,356 + BWI76_RS13045 0.53 -1.3 2,722,393 + BWI76_RS13045 0.62 +0.4 2,722,416 - BWI76_RS13045 0.68 +0.2 2,722,472 - BWI76_RS13045 0.82 -1.8 2,722,590 + -0.2 2,722,672 + +1.0 2,722,673 + +1.9 2,722,701 + -0.2 2,722,920 - BWI76_RS13050 0.53 -0.5 2,723,196 + -1.2 2,723,535 - BWI76_RS13055 0.31 -1.5 2,723,740 + BWI76_RS13055 0.53 -1.4 2,723,740 + BWI76_RS13055 0.53 -0.6 2,723,809 + BWI76_RS13055 0.61 +0.1 2,724,098 - -0.7 2,724,139 + -0.0 2,724,509 + BWI76_RS13060 0.19 -1.1 2,724,509 + BWI76_RS13060 0.19 -0.2 2,724,647 - BWI76_RS13060 0.42 -0.1 2,724,649 + BWI76_RS13060 0.42 -1.6 2,724,954 - +0.4
Or see this region's nucleotide sequence