Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12145 and BWI76_RS12150 are separated by 550 nucleotides BWI76_RS12150 and BWI76_RS12155 are separated by 78 nucleotides BWI76_RS12155 and BWI76_RS12160 are separated by 57 nucleotides BWI76_RS12160 and BWI76_RS12165 overlap by 1 nucleotides BWI76_RS12165 and BWI76_RS12170 are separated by 2 nucleotides
BWI76_RS12145: BWI76_RS12145 - hypothetical protein, at 2,546,659 to 2,547,765
_RS12145
BWI76_RS12150: BWI76_RS12150 - DNA damage-inducible protein I, at 2,548,316 to 2,548,549
_RS12150
BWI76_RS12155: BWI76_RS12155 - hypothetical protein, at 2,548,628 to 2,548,849
_RS12155
BWI76_RS12160: BWI76_RS12160 - hypothetical protein, at 2,548,907 to 2,549,506
_RS12160
BWI76_RS12165: BWI76_RS12165 - hypothetical protein, at 2,549,506 to 2,549,712
_RS12165
BWI76_RS12170: BWI76_RS12170 - hypothetical protein, at 2,549,715 to 2,550,011
_RS12170
Position (kb)
2548
2549 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2547.788 kb on + strand at 2547.875 kb on + strand at 2547.879 kb on + strand at 2547.883 kb on + strand at 2547.884 kb on + strand at 2547.902 kb on - strand at 2547.909 kb on + strand at 2547.909 kb on + strand at 2547.909 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.920 kb on + strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.928 kb on - strand at 2547.944 kb on + strand at 2547.959 kb on - strand at 2547.963 kb on - strand at 2547.969 kb on + strand at 2547.972 kb on + strand at 2547.977 kb on + strand at 2547.977 kb on + strand at 2547.977 kb on + strand at 2547.977 kb on + strand at 2547.977 kb on + strand at 2547.980 kb on - strand at 2547.985 kb on - strand at 2547.985 kb on - strand at 2547.990 kb on + strand at 2548.004 kb on - strand at 2548.004 kb on - strand at 2548.145 kb on + strand at 2548.145 kb on + strand at 2548.153 kb on - strand at 2548.306 kb on + strand at 2548.362 kb on + strand, within BWI76_RS12150 at 2548.373 kb on + strand, within BWI76_RS12150 at 2548.456 kb on + strand, within BWI76_RS12150 at 2548.457 kb on - strand, within BWI76_RS12150 at 2548.464 kb on - strand, within BWI76_RS12150 at 2548.471 kb on - strand, within BWI76_RS12150 at 2548.587 kb on + strand at 2548.608 kb on - strand at 2548.687 kb on - strand, within BWI76_RS12155 at 2548.687 kb on - strand, within BWI76_RS12155 at 2548.801 kb on + strand, within BWI76_RS12155 at 2548.801 kb on + strand, within BWI76_RS12155 at 2548.878 kb on + strand at 2548.890 kb on + strand at 2548.898 kb on + strand at 2548.925 kb on - strand at 2548.934 kb on - strand at 2548.944 kb on - strand at 2548.956 kb on + strand at 2548.969 kb on - strand, within BWI76_RS12160 at 2548.969 kb on - strand, within BWI76_RS12160 at 2548.970 kb on - strand, within BWI76_RS12160 at 2549.023 kb on + strand, within BWI76_RS12160 at 2549.023 kb on + strand, within BWI76_RS12160 at 2549.023 kb on + strand, within BWI76_RS12160 at 2549.031 kb on - strand, within BWI76_RS12160 at 2549.133 kb on + strand, within BWI76_RS12160 at 2549.134 kb on + strand, within BWI76_RS12160 at 2549.313 kb on - strand, within BWI76_RS12160 at 2549.452 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 2,547,788 + +0.1 2,547,875 + +1.3 2,547,879 + +0.4 2,547,883 + -0.3 2,547,884 + -1.1 2,547,902 - -0.5 2,547,909 + +0.5 2,547,909 + +0.4 2,547,909 + -0.5 2,547,920 + -1.8 2,547,920 + +0.5 2,547,920 + -0.3 2,547,920 + +0.3 2,547,920 + +0.0 2,547,920 + -1.0 2,547,920 + -0.4 2,547,920 + +0.3 2,547,920 + +2.2 2,547,920 + +1.5 2,547,920 + +0.6 2,547,920 + -0.0 2,547,920 + +0.2 2,547,920 + +0.9 2,547,920 + +0.6 2,547,928 - -1.1 2,547,928 - +1.5 2,547,928 - -0.9 2,547,928 - +0.5 2,547,928 - -0.2 2,547,928 - +1.1 2,547,944 + -0.9 2,547,959 - -0.2 2,547,963 - -0.9 2,547,969 + -0.4 2,547,972 + -0.4 2,547,977 + -0.2 2,547,977 + +0.2 2,547,977 + +0.2 2,547,977 + -0.3 2,547,977 + +0.6 2,547,980 - +0.3 2,547,985 - +0.0 2,547,985 - -0.9 2,547,990 + -1.2 2,548,004 - -1.1 2,548,004 - -2.3 2,548,145 + -0.8 2,548,145 + +0.1 2,548,153 - +1.0 2,548,306 + -0.6 2,548,362 + BWI76_RS12150 0.20 -1.3 2,548,373 + BWI76_RS12150 0.24 +0.1 2,548,456 + BWI76_RS12150 0.60 +0.1 2,548,457 - BWI76_RS12150 0.60 -1.6 2,548,464 - BWI76_RS12150 0.63 -3.4 2,548,471 - BWI76_RS12150 0.66 -0.7 2,548,587 + -0.1 2,548,608 - +0.1 2,548,687 - BWI76_RS12155 0.27 +1.5 2,548,687 - BWI76_RS12155 0.27 +0.8 2,548,801 + BWI76_RS12155 0.78 +1.1 2,548,801 + BWI76_RS12155 0.78 +0.5 2,548,878 + -0.0 2,548,890 + +0.5 2,548,898 + +0.6 2,548,925 - -1.1 2,548,934 - +1.2 2,548,944 - -0.1 2,548,956 + +0.5 2,548,969 - BWI76_RS12160 0.10 -2.9 2,548,969 - BWI76_RS12160 0.10 +1.4 2,548,970 - BWI76_RS12160 0.10 -1.8 2,549,023 + BWI76_RS12160 0.19 -1.5 2,549,023 + BWI76_RS12160 0.19 -1.1 2,549,023 + BWI76_RS12160 0.19 +0.5 2,549,031 - BWI76_RS12160 0.21 +0.5 2,549,133 + BWI76_RS12160 0.38 -1.7 2,549,134 + BWI76_RS12160 0.38 +0.5 2,549,313 - BWI76_RS12160 0.68 -0.4 2,549,452 - -2.6
Or see this region's nucleotide sequence