Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS12085 and BWI76_RS12090 are separated by 11 nucleotides BWI76_RS12090 and BWI76_RS12095 are separated by 332 nucleotides BWI76_RS12095 and BWI76_RS12100 overlap by 4 nucleotides BWI76_RS12100 and BWI76_RS12105 overlap by 8 nucleotides
BWI76_RS12085: BWI76_RS12085 - regulatory protein CII from bacteriophage origin, at 2,538,780 to 2,539,316
_RS12085
BWI76_RS12090: BWI76_RS12090 - HNH endonuclease, at 2,539,328 to 2,539,831
_RS12090
BWI76_RS12095: BWI76_RS12095 - hypothetical protein, at 2,540,164 to 2,541,084
_RS12095
BWI76_RS12100: BWI76_RS12100 - replication protein P, at 2,541,081 to 2,541,824
_RS12100
BWI76_RS12105: BWI76_RS12105 - hypothetical protein, at 2,541,817 to 2,542,152
_RS12105
Position (kb)
2540
2541
2542 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2539.183 kb on + strand, within BWI76_RS12085 at 2539.188 kb on + strand, within BWI76_RS12085 at 2539.258 kb on + strand, within BWI76_RS12085 at 2539.273 kb on - strand at 2539.278 kb on + strand at 2539.302 kb on - strand at 2539.339 kb on - strand at 2539.339 kb on - strand at 2539.500 kb on - strand, within BWI76_RS12090 at 2539.558 kb on + strand, within BWI76_RS12090 at 2539.566 kb on - strand, within BWI76_RS12090 at 2539.723 kb on + strand at 2539.890 kb on - strand at 2539.908 kb on - strand at 2540.163 kb on - strand at 2540.163 kb on - strand at 2540.166 kb on + strand at 2540.505 kb on + strand, within BWI76_RS12095 at 2540.758 kb on + strand, within BWI76_RS12095 at 2540.766 kb on + strand, within BWI76_RS12095 at 2540.766 kb on + strand, within BWI76_RS12095 at 2540.774 kb on - strand, within BWI76_RS12095 at 2540.774 kb on - strand, within BWI76_RS12095 at 2540.842 kb on - strand, within BWI76_RS12095 at 2541.008 kb on + strand at 2541.008 kb on + strand at 2541.016 kb on - strand at 2541.104 kb on + strand at 2541.133 kb on - strand at 2541.426 kb on - strand, within BWI76_RS12100 at 2541.524 kb on + strand, within BWI76_RS12100 at 2541.532 kb on - strand, within BWI76_RS12100 at 2541.618 kb on + strand, within BWI76_RS12100 at 2541.626 kb on - strand, within BWI76_RS12100 at 2541.636 kb on - strand, within BWI76_RS12100 at 2541.709 kb on + strand, within BWI76_RS12100 at 2542.011 kb on - strand, within BWI76_RS12105 at 2542.050 kb on + strand, within BWI76_RS12105
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 2,539,183 + BWI76_RS12085 0.75 +0.5 2,539,188 + BWI76_RS12085 0.76 +0.5 2,539,258 + BWI76_RS12085 0.89 -0.1 2,539,273 - +0.1 2,539,278 + -1.4 2,539,302 - +3.0 2,539,339 - -0.7 2,539,339 - -0.1 2,539,500 - BWI76_RS12090 0.34 +1.5 2,539,558 + BWI76_RS12090 0.46 +1.1 2,539,566 - BWI76_RS12090 0.47 -0.8 2,539,723 + +1.5 2,539,890 - +2.5 2,539,908 - +0.6 2,540,163 - +0.2 2,540,163 - +0.2 2,540,166 + -0.6 2,540,505 + BWI76_RS12095 0.37 -1.6 2,540,758 + BWI76_RS12095 0.64 -0.1 2,540,766 + BWI76_RS12095 0.65 -2.2 2,540,766 + BWI76_RS12095 0.65 -1.2 2,540,774 - BWI76_RS12095 0.66 +0.5 2,540,774 - BWI76_RS12095 0.66 -0.3 2,540,842 - BWI76_RS12095 0.74 -0.9 2,541,008 + -0.9 2,541,008 + +1.9 2,541,016 - +0.9 2,541,104 + -1.0 2,541,133 - -1.6 2,541,426 - BWI76_RS12100 0.46 +0.2 2,541,524 + BWI76_RS12100 0.60 +0.4 2,541,532 - BWI76_RS12100 0.61 +0.7 2,541,618 + BWI76_RS12100 0.72 +1.8 2,541,626 - BWI76_RS12100 0.73 -0.5 2,541,636 - BWI76_RS12100 0.75 -2.2 2,541,709 + BWI76_RS12100 0.84 -0.3 2,542,011 - BWI76_RS12105 0.58 -0.2 2,542,050 + BWI76_RS12105 0.69 +0.7
Or see this region's nucleotide sequence