Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS08270 and BWI76_RS08275 are separated by 50 nucleotides BWI76_RS08275 and BWI76_RS08280 are separated by 133 nucleotides BWI76_RS08280 and BWI76_RS08285 are separated by 184 nucleotides BWI76_RS08285 and BWI76_RS08290 are separated by 32 nucleotides
BWI76_RS08270: BWI76_RS08270 - flavodoxin, at 1,750,879 to 1,751,508
_RS08270
BWI76_RS08275: BWI76_RS08275 - LexA regulated protein, at 1,751,559 to 1,751,852
_RS08275
BWI76_RS08280: BWI76_RS08280 - acyl-CoA esterase, at 1,751,986 to 1,752,759
_RS08280
BWI76_RS08285: BWI76_RS08285 - replication initiation regulator SeqA, at 1,752,944 to 1,753,492
_RS08285
BWI76_RS08290: BWI76_RS08290 - phosphoglucomutase, alpha-D-glucose phosphate-specific, at 1,753,525 to 1,755,165
_RS08290
Position (kb)
1751
1752
1753 Strain fitness (log2 ratio)
-1
0
1 at 1751.518 kb on + strand at 1751.526 kb on - strand at 1751.571 kb on - strand at 1751.722 kb on + strand, within BWI76_RS08275 at 1751.754 kb on - strand, within BWI76_RS08275 at 1752.008 kb on - strand at 1752.163 kb on - strand, within BWI76_RS08280 at 1752.589 kb on + strand, within BWI76_RS08280 at 1752.605 kb on - strand, within BWI76_RS08280 at 1752.857 kb on + strand at 1752.862 kb on + strand at 1752.936 kb on + strand at 1753.237 kb on + strand, within BWI76_RS08285 at 1753.371 kb on - strand, within BWI76_RS08285
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 1,751,518 + +0.0 1,751,526 - -0.7 1,751,571 - +1.2 1,751,722 + BWI76_RS08275 0.55 +0.2 1,751,754 - BWI76_RS08275 0.66 -1.2 1,752,008 - -0.3 1,752,163 - BWI76_RS08280 0.23 -0.2 1,752,589 + BWI76_RS08280 0.78 +1.6 1,752,605 - BWI76_RS08280 0.80 +0.8 1,752,857 + +0.1 1,752,862 + +0.3 1,752,936 + -0.5 1,753,237 + BWI76_RS08285 0.53 +1.3 1,753,371 - BWI76_RS08285 0.78 +1.3
Or see this region's nucleotide sequence