Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS07050 and BWI76_RS07055 are separated by 10 nucleotides BWI76_RS07055 and BWI76_RS07060 are separated by 9 nucleotides BWI76_RS07060 and BWI76_RS07065 are separated by 220 nucleotides
BWI76_RS07050: BWI76_RS07050 - putative cytoplasmic protein, at 1,483,353 to 1,484,612
_RS07050
BWI76_RS07055: BWI76_RS07055 - putative cytoplasmic protein, at 1,484,623 to 1,485,426
_RS07055
BWI76_RS07060: BWI76_RS07060 - carbamate kinase, at 1,485,436 to 1,486,329
_RS07060
BWI76_RS07065: BWI76_RS07065 - 5-(carboxyamino)imidazole ribonucleotide synthase, at 1,486,550 to 1,487,617
_RS07065
Position (kb)
1485
1486
1487 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1484.473 kb on + strand, within BWI76_RS07050 at 1484.606 kb on + strand at 1484.693 kb on + strand at 1484.753 kb on + strand, within BWI76_RS07055 at 1484.761 kb on - strand, within BWI76_RS07055 at 1484.877 kb on + strand, within BWI76_RS07055 at 1485.024 kb on - strand, within BWI76_RS07055 at 1485.050 kb on + strand, within BWI76_RS07055 at 1485.056 kb on + strand, within BWI76_RS07055 at 1485.238 kb on + strand, within BWI76_RS07055 at 1485.246 kb on - strand, within BWI76_RS07055 at 1485.246 kb on - strand, within BWI76_RS07055 at 1485.485 kb on + strand at 1485.605 kb on - strand, within BWI76_RS07060 at 1485.928 kb on - strand, within BWI76_RS07060 at 1486.079 kb on - strand, within BWI76_RS07060 at 1486.271 kb on - strand at 1486.360 kb on + strand at 1486.362 kb on + strand at 1486.540 kb on - strand at 1486.753 kb on - strand, within BWI76_RS07065 at 1486.967 kb on - strand, within BWI76_RS07065 at 1487.019 kb on + strand, within BWI76_RS07065 at 1487.154 kb on - strand, within BWI76_RS07065 at 1487.222 kb on + strand, within BWI76_RS07065 at 1487.293 kb on - strand, within BWI76_RS07065 at 1487.293 kb on - strand, within BWI76_RS07065
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 1,484,473 + BWI76_RS07050 0.89 +1.0 1,484,606 + +0.1 1,484,693 + -0.7 1,484,753 + BWI76_RS07055 0.16 +1.0 1,484,761 - BWI76_RS07055 0.17 -0.2 1,484,877 + BWI76_RS07055 0.32 -0.2 1,485,024 - BWI76_RS07055 0.50 -0.4 1,485,050 + BWI76_RS07055 0.53 +0.7 1,485,056 + BWI76_RS07055 0.54 -0.6 1,485,238 + BWI76_RS07055 0.76 -0.1 1,485,246 - BWI76_RS07055 0.77 -3.7 1,485,246 - BWI76_RS07055 0.77 +0.1 1,485,485 + +1.0 1,485,605 - BWI76_RS07060 0.19 +0.2 1,485,928 - BWI76_RS07060 0.55 +0.8 1,486,079 - BWI76_RS07060 0.72 +1.0 1,486,271 - -1.1 1,486,360 + -0.4 1,486,362 + -0.2 1,486,540 - +0.1 1,486,753 - BWI76_RS07065 0.19 -0.1 1,486,967 - BWI76_RS07065 0.39 -0.5 1,487,019 + BWI76_RS07065 0.44 +0.4 1,487,154 - BWI76_RS07065 0.57 -0.9 1,487,222 + BWI76_RS07065 0.63 -1.1 1,487,293 - BWI76_RS07065 0.70 -1.0 1,487,293 - BWI76_RS07065 0.70 -0.2
Or see this region's nucleotide sequence