Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05130 and BWI76_RS05135 overlap by 1 nucleotides BWI76_RS05135 and BWI76_RS05140 overlap by 4 nucleotides BWI76_RS05140 and BWI76_RS05145 are separated by 118 nucleotides
BWI76_RS05130: BWI76_RS05130 - iron-hydroxamate transporter ATP-binding subunit, at 1,078,385 to 1,079,182
_RS05130
BWI76_RS05135: BWI76_RS05135 - iron-hydroxamate transporter substrate-binding subunit, at 1,079,182 to 1,080,072
_RS05135
BWI76_RS05140: BWI76_RS05140 - Fe3+-hydroxamate ABC transporter permease FhuB, at 1,080,069 to 1,082,051
_RS05140
BWI76_RS05145: BWI76_RS05145 - aspartate aminotransferase family protein, at 1,082,170 to 1,083,450
_RS05145
Position (kb) 1080
1081
1082
1083 Strain fitness (log2 ratio) -1
0
1 at 1079.425 kb on + strand, within BWI76_RS05135 at 1079.502 kb on - strand, within BWI76_RS05135 at 1081.107 kb on + strand, within BWI76_RS05140 at 1081.190 kb on - strand, within BWI76_RS05140 at 1081.242 kb on - strand, within BWI76_RS05140 at 1081.299 kb on + strand, within BWI76_RS05140 at 1081.338 kb on - strand, within BWI76_RS05140 at 1081.557 kb on + strand, within BWI76_RS05140 at 1082.143 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 1,079,425 + BWI76_RS05135 0.27 +0.2 1,079,502 - BWI76_RS05135 0.36 +0.0 1,081,107 + BWI76_RS05140 0.52 +1.3 1,081,190 - BWI76_RS05140 0.57 +1.4 1,081,242 - BWI76_RS05140 0.59 +0.6 1,081,299 + BWI76_RS05140 0.62 +0.4 1,081,338 - BWI76_RS05140 0.64 +1.0 1,081,557 + BWI76_RS05140 0.75 -0.5 1,082,143 - +0.2
Or see this region's nucleotide sequence