Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS05050 and BWI76_RS05055 overlap by 8 nucleotides BWI76_RS05055 and BWI76_RS05060 overlap by 4 nucleotides BWI76_RS05060 and BWI76_RS05065 overlap by 4 nucleotides BWI76_RS05065 and BWI76_RS05070 overlap by 4 nucleotides BWI76_RS05070 and BWI76_RS05075 overlap by 1 nucleotides
BWI76_RS05050: BWI76_RS05050 - general secretion pathway protein GspL, at 1,058,509 to 1,059,705
_RS05050
BWI76_RS05055: BWI76_RS05055 - type II secretion system protein K, at 1,059,698 to 1,060,678
_RS05055
BWI76_RS05060: BWI76_RS05060 - type II secretion system protein GspJ, at 1,060,675 to 1,061,271
_RS05060
BWI76_RS05065: BWI76_RS05065 - type II secretion system protein GspI, at 1,061,268 to 1,061,633
_RS05065
BWI76_RS05070: BWI76_RS05070 - type II secretion system protein GspH, at 1,061,630 to 1,062,142
_RS05070
BWI76_RS05075: BWI76_RS05075 - type II secretion system protein GspG, at 1,062,142 to 1,062,564
_RS05075
Position (kb)
1060
1061
1062 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1059.797 kb on + strand, within BWI76_RS05055 at 1059.970 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.020 kb on + strand, within BWI76_RS05055 at 1060.106 kb on - strand, within BWI76_RS05055 at 1060.181 kb on - strand, within BWI76_RS05055 at 1060.242 kb on + strand, within BWI76_RS05055 at 1060.267 kb on + strand, within BWI76_RS05055 at 1060.311 kb on + strand, within BWI76_RS05055 at 1060.348 kb on + strand, within BWI76_RS05055 at 1060.371 kb on - strand, within BWI76_RS05055 at 1060.438 kb on + strand at 1060.445 kb on + strand, within BWI76_RS05055 at 1060.453 kb on - strand, within BWI76_RS05055 at 1060.607 kb on + strand at 1060.649 kb on - strand at 1060.776 kb on + strand, within BWI76_RS05060 at 1060.835 kb on + strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.850 kb on - strand, within BWI76_RS05060 at 1060.854 kb on + strand, within BWI76_RS05060 at 1060.879 kb on + strand, within BWI76_RS05060 at 1060.979 kb on - strand, within BWI76_RS05060 at 1061.022 kb on + strand, within BWI76_RS05060 at 1061.244 kb on + strand at 1061.244 kb on + strand at 1061.244 kb on + strand at 1061.252 kb on - strand at 1061.451 kb on - strand, within BWI76_RS05065 at 1061.780 kb on - strand, within BWI76_RS05070 at 1062.031 kb on - strand, within BWI76_RS05070 at 1062.129 kb on - strand at 1062.201 kb on + strand, within BWI76_RS05075
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 1,059,797 + BWI76_RS05055 0.10 +0.6 1,059,970 + BWI76_RS05055 0.28 +0.4 1,060,020 + BWI76_RS05055 0.33 -0.0 1,060,020 + BWI76_RS05055 0.33 +0.5 1,060,020 + BWI76_RS05055 0.33 +1.8 1,060,106 - BWI76_RS05055 0.42 +0.8 1,060,181 - BWI76_RS05055 0.49 +0.2 1,060,242 + BWI76_RS05055 0.55 +0.7 1,060,267 + BWI76_RS05055 0.58 -0.4 1,060,311 + BWI76_RS05055 0.62 +0.9 1,060,348 + BWI76_RS05055 0.66 +1.5 1,060,371 - BWI76_RS05055 0.69 +0.1 1,060,438 + +1.9 1,060,445 + BWI76_RS05055 0.76 +1.2 1,060,453 - BWI76_RS05055 0.77 +0.5 1,060,607 + -0.3 1,060,649 - -0.2 1,060,776 + BWI76_RS05060 0.17 -2.2 1,060,835 + BWI76_RS05060 0.27 +2.2 1,060,850 - BWI76_RS05060 0.29 +1.3 1,060,850 - BWI76_RS05060 0.29 +1.2 1,060,850 - BWI76_RS05060 0.29 +0.4 1,060,854 + BWI76_RS05060 0.30 +1.0 1,060,879 + BWI76_RS05060 0.34 +1.4 1,060,979 - BWI76_RS05060 0.51 +1.5 1,061,022 + BWI76_RS05060 0.58 +0.5 1,061,244 + -1.3 1,061,244 + +0.3 1,061,244 + +0.5 1,061,252 - -0.1 1,061,451 - BWI76_RS05065 0.50 -0.9 1,061,780 - BWI76_RS05070 0.29 +0.6 1,062,031 - BWI76_RS05070 0.78 -0.3 1,062,129 - -0.6 1,062,201 + BWI76_RS05075 0.14 -0.2
Or see this region's nucleotide sequence