Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS04395 and BWI76_RS04400 are separated by 92 nucleotides BWI76_RS04400 and BWI76_RS04405 are separated by 44 nucleotides BWI76_RS04405 and BWI76_RS04410 overlap by 8 nucleotides
BWI76_RS04390: BWI76_RS04390 - DUF805 domain-containing protein, at 906,636 to 906,998
_RS04390
BWI76_RS04395: BWI76_RS04395 - putative HTH-type transcriptional regulator, at 906,931 to 907,875
_RS04395
BWI76_RS04400: BWI76_RS04400 - alanyl-tRNA synthetase, at 907,968 to 908,597
_RS04400
BWI76_RS04405: BWI76_RS04405 - response regulator, at 908,642 to 909,340
_RS04405
BWI76_RS04410: BWI76_RS04410 - signal transduction histidine kinase regulating citrate/malate metabolism, at 909,333 to 910,961
_RS04410
Position (kb) 907
908
909 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 906.968 kb on - strand at 906.978 kb on - strand at 907.036 kb on - strand, within BWI76_RS04395 at 907.055 kb on + strand, within BWI76_RS04395 at 907.060 kb on - strand, within BWI76_RS04395 at 907.073 kb on - strand, within BWI76_RS04395 at 907.197 kb on + strand, within BWI76_RS04395 at 907.425 kb on + strand, within BWI76_RS04395 at 907.457 kb on - strand, within BWI76_RS04395 at 907.735 kb on + strand, within BWI76_RS04395 at 907.749 kb on - strand, within BWI76_RS04395 at 907.957 kb on + strand at 907.964 kb on + strand at 908.164 kb on + strand, within BWI76_RS04400 at 908.177 kb on - strand, within BWI76_RS04400 at 908.247 kb on + strand, within BWI76_RS04400 at 908.273 kb on + strand at 908.321 kb on - strand, within BWI76_RS04400 at 908.336 kb on - strand, within BWI76_RS04400 at 908.365 kb on - strand, within BWI76_RS04400 at 908.497 kb on - strand, within BWI76_RS04400 at 908.509 kb on + strand, within BWI76_RS04400 at 908.595 kb on - strand at 908.740 kb on + strand, within BWI76_RS04405 at 908.753 kb on - strand, within BWI76_RS04405 at 908.753 kb on - strand, within BWI76_RS04405 at 908.836 kb on - strand, within BWI76_RS04405 at 908.843 kb on + strand, within BWI76_RS04405 at 908.850 kb on - strand, within BWI76_RS04405 at 908.855 kb on + strand, within BWI76_RS04405 at 908.910 kb on + strand, within BWI76_RS04405 at 908.956 kb on + strand, within BWI76_RS04405 at 909.112 kb on + strand, within BWI76_RS04405 at 909.170 kb on - strand, within BWI76_RS04405 at 909.186 kb on + strand, within BWI76_RS04405 at 909.204 kb on - strand, within BWI76_RS04405 at 909.238 kb on - strand, within BWI76_RS04405 at 909.257 kb on - strand, within BWI76_RS04405 at 909.273 kb on + strand at 909.298 kb on + strand at 909.306 kb on - strand at 909.325 kb on + strand at 909.333 kb on - strand at 909.447 kb on - strand at 909.449 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 906,968 - -0.5 906,978 - +0.3 907,036 - BWI76_RS04395 0.11 +0.1 907,055 + BWI76_RS04395 0.13 -0.4 907,060 - BWI76_RS04395 0.14 -0.4 907,073 - BWI76_RS04395 0.15 -0.9 907,197 + BWI76_RS04395 0.28 +0.7 907,425 + BWI76_RS04395 0.52 +0.8 907,457 - BWI76_RS04395 0.56 -1.9 907,735 + BWI76_RS04395 0.85 -0.3 907,749 - BWI76_RS04395 0.87 -0.5 907,957 + -0.5 907,964 + +0.1 908,164 + BWI76_RS04400 0.31 -0.6 908,177 - BWI76_RS04400 0.33 +1.0 908,247 + BWI76_RS04400 0.44 +0.0 908,273 + +2.2 908,321 - BWI76_RS04400 0.56 +0.7 908,336 - BWI76_RS04400 0.58 -0.1 908,365 - BWI76_RS04400 0.63 +0.6 908,497 - BWI76_RS04400 0.84 +1.6 908,509 + BWI76_RS04400 0.86 +2.5 908,595 - -0.3 908,740 + BWI76_RS04405 0.14 -0.2 908,753 - BWI76_RS04405 0.16 -0.3 908,753 - BWI76_RS04405 0.16 +1.3 908,836 - BWI76_RS04405 0.28 +0.2 908,843 + BWI76_RS04405 0.29 -1.7 908,850 - BWI76_RS04405 0.30 -0.1 908,855 + BWI76_RS04405 0.30 -0.1 908,910 + BWI76_RS04405 0.38 +0.1 908,956 + BWI76_RS04405 0.45 -0.1 909,112 + BWI76_RS04405 0.67 -0.5 909,170 - BWI76_RS04405 0.76 +3.1 909,186 + BWI76_RS04405 0.78 -0.4 909,204 - BWI76_RS04405 0.80 -1.3 909,238 - BWI76_RS04405 0.85 -1.0 909,257 - BWI76_RS04405 0.88 -0.3 909,273 + -0.0 909,298 + +0.4 909,306 - +0.1 909,325 + +0.4 909,333 - -0.6 909,447 - +0.2 909,449 + -0.2
Or see this region's nucleotide sequence