Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS00325 and BWI76_RS00330 overlap by 4 nucleotides BWI76_RS00330 and BWI76_RS00335 are separated by 71 nucleotides BWI76_RS00335 and BWI76_RS00340 are separated by 75 nucleotides
BWI76_RS00325: BWI76_RS00325 - molybdopterin-guanine dinucleotide biosynthesis protein MobB, at 69,712 to 70,218
_RS00325
BWI76_RS00330: BWI76_RS00330 - molybdenum cofactor guanylyltransferase, at 70,215 to 70,796
_RS00330
BWI76_RS00335: BWI76_RS00335 - hypothetical protein, at 70,868 to 71,137
_RS00335
BWI76_RS00340: BWI76_RS00340 - stress response kinase A, at 71,213 to 72,199
_RS00340
Position (kb) 70
71
72 Strain fitness (log2 ratio) -1
0
1
2 at 69.926 kb on - strand, within BWI76_RS00325 at 70.056 kb on + strand, within BWI76_RS00325 at 70.124 kb on - strand, within BWI76_RS00325 at 70.364 kb on - strand, within BWI76_RS00330 at 70.386 kb on + strand, within BWI76_RS00330 at 70.525 kb on + strand, within BWI76_RS00330 at 70.791 kb on - strand at 70.829 kb on - strand at 70.835 kb on + strand at 70.844 kb on - strand at 70.913 kb on + strand, within BWI76_RS00335 at 71.051 kb on - strand, within BWI76_RS00335 at 71.115 kb on - strand at 71.188 kb on + strand at 71.224 kb on + strand at 71.232 kb on - strand at 71.303 kb on - strand at 71.317 kb on - strand, within BWI76_RS00340 at 71.529 kb on + strand, within BWI76_RS00340 at 71.637 kb on - strand, within BWI76_RS00340 at 72.120 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 69,926 - BWI76_RS00325 0.42 -0.5 70,056 + BWI76_RS00325 0.68 -1.4 70,124 - BWI76_RS00325 0.81 -0.8 70,364 - BWI76_RS00330 0.26 +0.4 70,386 + BWI76_RS00330 0.29 -0.0 70,525 + BWI76_RS00330 0.53 -0.5 70,791 - -0.4 70,829 - -0.5 70,835 + +2.3 70,844 - -0.6 70,913 + BWI76_RS00335 0.17 -1.0 71,051 - BWI76_RS00335 0.68 -0.4 71,115 - -0.3 71,188 + -0.7 71,224 + +0.1 71,232 - -0.9 71,303 - -1.7 71,317 - BWI76_RS00340 0.11 -0.5 71,529 + BWI76_RS00340 0.32 -0.4 71,637 - BWI76_RS00340 0.43 -0.3 72,120 + +0.1
Or see this region's nucleotide sequence