Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20965 and BWI76_RS20970 overlap by 4 nucleotides BWI76_RS20970 and BWI76_RS20975 are separated by 43 nucleotides BWI76_RS20975 and BWI76_RS20980 are separated by 422 nucleotides
BWI76_RS20965: BWI76_RS20965 - phosphoribosylformylglycinamidine cyclo-ligase, at 4,382,370 to 4,383,407
_RS20965
BWI76_RS20970: BWI76_RS20970 - phosphoribosylglycinamide formyltransferase, at 4,383,404 to 4,384,045
_RS20970
BWI76_RS20975: BWI76_RS20975 - hypothetical protein, at 4,384,089 to 4,385,447
_RS20975
BWI76_RS20980: BWI76_RS20980 - MFS transporter, at 4,385,870 to 4,387,324
_RS20980
Position (kb) 4384
4385
4386 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4383.163 kb on - strand, within BWI76_RS20965 at 4383.337 kb on - strand at 4383.346 kb on - strand at 4383.379 kb on + strand at 4383.431 kb on + strand at 4383.859 kb on + strand, within BWI76_RS20970 at 4384.255 kb on + strand, within BWI76_RS20975 at 4384.435 kb on - strand, within BWI76_RS20975 at 4384.529 kb on - strand, within BWI76_RS20975 at 4384.759 kb on - strand, within BWI76_RS20975 at 4384.787 kb on - strand, within BWI76_RS20975 at 4384.884 kb on + strand, within BWI76_RS20975 at 4384.922 kb on - strand, within BWI76_RS20975 at 4384.923 kb on - strand, within BWI76_RS20975 at 4384.953 kb on - strand, within BWI76_RS20975 at 4384.978 kb on + strand, within BWI76_RS20975 at 4384.983 kb on + strand, within BWI76_RS20975 at 4385.068 kb on - strand, within BWI76_RS20975 at 4385.205 kb on + strand, within BWI76_RS20975 at 4385.213 kb on - strand, within BWI76_RS20975 at 4385.213 kb on - strand, within BWI76_RS20975 at 4385.231 kb on - strand, within BWI76_RS20975 at 4385.407 kb on + strand at 4385.408 kb on - strand at 4385.512 kb on + strand at 4385.515 kb on - strand at 4385.522 kb on + strand at 4385.651 kb on + strand at 4385.717 kb on + strand at 4385.717 kb on + strand at 4385.717 kb on + strand at 4385.764 kb on + strand at 4385.785 kb on - strand at 4385.820 kb on + strand at 4385.863 kb on - strand at 4385.966 kb on + strand at 4386.027 kb on - strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.104 kb on + strand, within BWI76_RS20980 at 4386.107 kb on - strand, within BWI76_RS20980 at 4386.112 kb on - strand, within BWI76_RS20980 at 4386.117 kb on - strand, within BWI76_RS20980 at 4386.143 kb on - strand, within BWI76_RS20980 at 4386.187 kb on - strand, within BWI76_RS20980 at 4386.195 kb on + strand, within BWI76_RS20980 at 4386.195 kb on + strand, within BWI76_RS20980 at 4386.195 kb on + strand, within BWI76_RS20980 at 4386.203 kb on - strand, within BWI76_RS20980 at 4386.203 kb on - strand, within BWI76_RS20980 at 4386.203 kb on - strand, within BWI76_RS20980 at 4386.272 kb on - strand, within BWI76_RS20980 at 4386.369 kb on - strand, within BWI76_RS20980 at 4386.374 kb on + strand, within BWI76_RS20980 at 4386.393 kb on - strand, within BWI76_RS20980 at 4386.408 kb on + strand, within BWI76_RS20980
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 4,383,163 - BWI76_RS20965 0.76 -0.1 4,383,337 - -0.4 4,383,346 - -0.3 4,383,379 + +0.2 4,383,431 + -0.1 4,383,859 + BWI76_RS20970 0.71 -0.5 4,384,255 + BWI76_RS20975 0.12 +0.2 4,384,435 - BWI76_RS20975 0.25 -0.5 4,384,529 - BWI76_RS20975 0.32 +0.6 4,384,759 - BWI76_RS20975 0.49 +1.1 4,384,787 - BWI76_RS20975 0.51 +0.2 4,384,884 + BWI76_RS20975 0.58 -0.9 4,384,922 - BWI76_RS20975 0.61 -0.1 4,384,923 - BWI76_RS20975 0.61 -0.8 4,384,953 - BWI76_RS20975 0.64 -1.3 4,384,978 + BWI76_RS20975 0.65 -0.3 4,384,983 + BWI76_RS20975 0.66 +1.1 4,385,068 - BWI76_RS20975 0.72 +0.0 4,385,205 + BWI76_RS20975 0.82 -0.1 4,385,213 - BWI76_RS20975 0.83 +0.9 4,385,213 - BWI76_RS20975 0.83 +0.1 4,385,231 - BWI76_RS20975 0.84 -2.1 4,385,407 + -1.3 4,385,408 - +0.2 4,385,512 + -1.3 4,385,515 - -0.5 4,385,522 + -0.1 4,385,651 + -0.0 4,385,717 + -0.0 4,385,717 + -0.1 4,385,717 + -0.2 4,385,764 + -0.7 4,385,785 - +0.3 4,385,820 + -0.1 4,385,863 - -0.2 4,385,966 + +1.8 4,386,027 - BWI76_RS20980 0.11 -0.4 4,386,104 + BWI76_RS20980 0.16 -1.4 4,386,104 + BWI76_RS20980 0.16 +0.0 4,386,104 + BWI76_RS20980 0.16 +0.1 4,386,104 + BWI76_RS20980 0.16 +0.1 4,386,104 + BWI76_RS20980 0.16 -0.5 4,386,107 - BWI76_RS20980 0.16 +0.4 4,386,112 - BWI76_RS20980 0.17 +0.7 4,386,117 - BWI76_RS20980 0.17 -0.6 4,386,143 - BWI76_RS20980 0.19 +0.1 4,386,187 - BWI76_RS20980 0.22 +0.3 4,386,195 + BWI76_RS20980 0.22 -1.4 4,386,195 + BWI76_RS20980 0.22 +0.5 4,386,195 + BWI76_RS20980 0.22 +0.3 4,386,203 - BWI76_RS20980 0.23 -0.1 4,386,203 - BWI76_RS20980 0.23 +0.1 4,386,203 - BWI76_RS20980 0.23 -0.3 4,386,272 - BWI76_RS20980 0.28 -0.2 4,386,369 - BWI76_RS20980 0.34 -0.5 4,386,374 + BWI76_RS20980 0.35 +0.9 4,386,393 - BWI76_RS20980 0.36 +0.7 4,386,408 + BWI76_RS20980 0.37 +0.7
Or see this region's nucleotide sequence