Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS20520 and BWI76_RS20525 are separated by 176 nucleotides BWI76_RS20525 and BWI76_RS20530 are separated by 130 nucleotides BWI76_RS20530 and BWI76_RS20535 are separated by 48 nucleotides
BWI76_RS20520: BWI76_RS20520 - indolepyruvate decarboxylase, at 4,297,529 to 4,299,190
_RS20520
BWI76_RS20525: BWI76_RS20525 - glyceraldehyde 3-phosphate reductase, at 4,299,367 to 4,300,359
_RS20525
BWI76_RS20530: BWI76_RS20530 - hypothetical protein, at 4,300,490 to 4,300,849
_RS20530
BWI76_RS20535: BWI76_RS20535 - manganese transporter MntH, at 4,300,898 to 4,302,133
_RS20535
Position (kb)
4299
4300
4301 Strain fitness (log2 ratio)
-2
-1
0
1 at 4298.629 kb on + strand, within BWI76_RS20520 at 4298.672 kb on - strand, within BWI76_RS20520 at 4298.885 kb on - strand, within BWI76_RS20520 at 4298.905 kb on - strand, within BWI76_RS20520 at 4298.914 kb on - strand, within BWI76_RS20520 at 4299.045 kb on - strand at 4299.245 kb on + strand at 4299.253 kb on - strand at 4299.253 kb on - strand at 4299.253 kb on - strand at 4299.386 kb on - strand at 4299.756 kb on - strand, within BWI76_RS20525 at 4299.825 kb on + strand, within BWI76_RS20525 at 4299.979 kb on - strand, within BWI76_RS20525 at 4300.019 kb on + strand, within BWI76_RS20525 at 4300.207 kb on + strand, within BWI76_RS20525 at 4300.215 kb on - strand, within BWI76_RS20525 at 4300.215 kb on - strand, within BWI76_RS20525 at 4300.241 kb on + strand, within BWI76_RS20525 at 4300.275 kb on + strand at 4300.417 kb on - strand at 4300.434 kb on + strand at 4300.447 kb on + strand at 4300.488 kb on - strand at 4300.563 kb on + strand, within BWI76_RS20530 at 4300.704 kb on - strand, within BWI76_RS20530 at 4300.726 kb on + strand, within BWI76_RS20530
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 4,298,629 + BWI76_RS20520 0.66 +1.6 4,298,672 - BWI76_RS20520 0.69 -0.6 4,298,885 - BWI76_RS20520 0.82 +1.0 4,298,905 - BWI76_RS20520 0.83 +1.3 4,298,914 - BWI76_RS20520 0.83 +1.0 4,299,045 - +1.0 4,299,245 + -0.1 4,299,253 - -0.2 4,299,253 - -0.7 4,299,253 - -1.3 4,299,386 - -0.1 4,299,756 - BWI76_RS20525 0.39 +0.1 4,299,825 + BWI76_RS20525 0.46 -1.3 4,299,979 - BWI76_RS20525 0.62 -0.1 4,300,019 + BWI76_RS20525 0.66 +0.2 4,300,207 + BWI76_RS20525 0.85 -0.4 4,300,215 - BWI76_RS20525 0.85 -0.7 4,300,215 - BWI76_RS20525 0.85 -0.5 4,300,241 + BWI76_RS20525 0.88 +0.2 4,300,275 + -0.2 4,300,417 - -2.2 4,300,434 + +0.6 4,300,447 + +0.4 4,300,488 - +0.5 4,300,563 + BWI76_RS20530 0.20 -0.8 4,300,704 - BWI76_RS20530 0.59 -0.7 4,300,726 + BWI76_RS20530 0.66 +0.1
Or see this region's nucleotide sequence