Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS16170

Experiment: KLEB15 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS16165 and BWI76_RS16170 overlap by 1 nucleotidesBWI76_RS16170 and BWI76_RS16175 are separated by 111 nucleotides BWI76_RS16165: BWI76_RS16165 - peptidase M20, at 3,353,027 to 3,354,337 _RS16165 BWI76_RS16170: BWI76_RS16170 - amidohydrolase, at 3,354,337 to 3,355,782 _RS16170 BWI76_RS16175: BWI76_RS16175 - p-aminobenzoyl-glutamate transporter, at 3,355,894 to 3,357,420 _RS16175 Position (kb) 3354 3355 3356Strain fitness (log2 ratio) -2 -1 0 1at 3353.544 kb on - strand, within BWI76_RS16165at 3353.690 kb on + strand, within BWI76_RS16165at 3353.833 kb on - strand, within BWI76_RS16165at 3354.553 kb on - strand, within BWI76_RS16170at 3355.081 kb on + strand, within BWI76_RS16170at 3355.197 kb on - strand, within BWI76_RS16170at 3355.441 kb on - strand, within BWI76_RS16170at 3355.724 kb on - strandat 3355.724 kb on - strandat 3355.766 kb on - strandat 3355.905 kb on + strandat 3355.912 kb on + strandat 3355.943 kb on - strandat 3356.652 kb on + strand, within BWI76_RS16175at 3356.652 kb on + strand, within BWI76_RS16175at 3356.677 kb on - strand, within BWI76_RS16175at 3356.680 kb on + strand, within BWI76_RS16175

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 1
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3,353,544 - BWI76_RS16165 0.39 +0.2
3,353,690 + BWI76_RS16165 0.51 -0.6
3,353,833 - BWI76_RS16165 0.61 -0.7
3,354,553 - BWI76_RS16170 0.15 -1.4
3,355,081 + BWI76_RS16170 0.51 -0.9
3,355,197 - BWI76_RS16170 0.59 -1.9
3,355,441 - BWI76_RS16170 0.76 -0.6
3,355,724 - -0.1
3,355,724 - -0.7
3,355,766 - +0.3
3,355,905 + +1.0
3,355,912 + -0.4
3,355,943 - -1.3
3,356,652 + BWI76_RS16175 0.50 -0.1
3,356,652 + BWI76_RS16175 0.50 -0.8
3,356,677 - BWI76_RS16175 0.51 +1.1
3,356,680 + BWI76_RS16175 0.51 +0.6

Or see this region's nucleotide sequence