Strain Fitness in Klebsiella michiganensis M5al around BWI76_RS14625

Experiment: KLEB15 MOI 1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntBWI76_RS14620 and BWI76_RS14625 are separated by 171 nucleotidesBWI76_RS14625 and BWI76_RS14630 are separated by 51 nucleotides BWI76_RS14620: BWI76_RS14620 - acetylornithine deacetylase or succinyl-diaminopimelate desuccinylase, at 3,068,313 to 3,069,425 _RS14620 BWI76_RS14625: BWI76_RS14625 - protein TolA, at 3,069,597 to 3,070,025 _RS14625 BWI76_RS14630: BWI76_RS14630 - TonB-dependent receptor, at 3,070,077 to 3,072,041 _RS14630 Position (kb) 3069 3070 3071Strain fitness (log2 ratio) -1 0 1at 3068.897 kb on + strand, within BWI76_RS14620at 3069.389 kb on + strandat 3069.850 kb on - strand, within BWI76_RS14625at 3070.008 kb on - strandat 3070.193 kb on + strandat 3070.506 kb on - strand, within BWI76_RS14630at 3070.637 kb on + strand, within BWI76_RS14630at 3070.851 kb on - strand, within BWI76_RS14630at 3070.858 kb on - strand, within BWI76_RS14630

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Per-strain Table

Position Strand Gene LocusTag Fraction KLEB15 MOI 1
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3,068,897 + BWI76_RS14620 0.52 +0.1
3,069,389 + -0.8
3,069,850 - BWI76_RS14625 0.59 +1.7
3,070,008 - -1.2
3,070,193 + +0.6
3,070,506 - BWI76_RS14630 0.22 -1.4
3,070,637 + BWI76_RS14630 0.28 -0.5
3,070,851 - BWI76_RS14630 0.39 -1.0
3,070,858 - BWI76_RS14630 0.40 +0.8

Or see this region's nucleotide sequence