Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS14455 and BWI76_RS14460 are separated by 132 nucleotides BWI76_RS14460 and BWI76_RS14465 are separated by 329 nucleotides BWI76_RS14465 and BWI76_RS14470 are separated by 67 nucleotides
BWI76_RS14455: BWI76_RS14455 - aspartate aminotransferase family protein, at 3,015,936 to 3,017,279
_RS14455
BWI76_RS14460: BWI76_RS14460 - MurR/RpiR family transcriptional regulator, at 3,017,412 to 3,018,269
_RS14460
BWI76_RS14465: BWI76_RS14465 - transcriptional regulator, at 3,018,599 to 3,019,042
_RS14465
BWI76_RS14470: BWI76_RS14470 - L-serine ammonia-lyase, at 3,019,110 to 3,020,477
_RS14470
Position (kb)
3017
3018
3019 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3016.431 kb on + strand, within BWI76_RS14455 at 3016.439 kb on - strand, within BWI76_RS14455 at 3016.509 kb on - strand, within BWI76_RS14455 at 3016.509 kb on - strand, within BWI76_RS14455 at 3016.630 kb on - strand, within BWI76_RS14455 at 3017.085 kb on + strand, within BWI76_RS14455 at 3017.087 kb on - strand, within BWI76_RS14455 at 3017.187 kb on + strand at 3017.245 kb on - strand at 3017.972 kb on - strand, within BWI76_RS14460 at 3018.128 kb on + strand, within BWI76_RS14460 at 3018.128 kb on + strand, within BWI76_RS14460 at 3018.223 kb on + strand at 3018.286 kb on - strand at 3018.324 kb on - strand at 3018.375 kb on - strand at 3018.387 kb on - strand at 3018.395 kb on - strand at 3018.422 kb on - strand at 3018.614 kb on + strand at 3018.617 kb on - strand at 3018.617 kb on - strand at 3018.643 kb on + strand at 3018.671 kb on + strand, within BWI76_RS14465 at 3018.671 kb on + strand, within BWI76_RS14465 at 3018.726 kb on + strand, within BWI76_RS14465 at 3018.759 kb on + strand, within BWI76_RS14465 at 3018.967 kb on + strand, within BWI76_RS14465 at 3018.968 kb on + strand, within BWI76_RS14465 at 3018.970 kb on + strand, within BWI76_RS14465 at 3018.983 kb on + strand, within BWI76_RS14465 at 3018.983 kb on + strand, within BWI76_RS14465 at 3018.983 kb on + strand, within BWI76_RS14465 at 3018.991 kb on - strand, within BWI76_RS14465 at 3019.003 kb on - strand at 3019.004 kb on - strand at 3019.050 kb on + strand at 3019.218 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 3,016,431 + BWI76_RS14455 0.37 -0.8 3,016,439 - BWI76_RS14455 0.37 -0.7 3,016,509 - BWI76_RS14455 0.43 -0.3 3,016,509 - BWI76_RS14455 0.43 +0.1 3,016,630 - BWI76_RS14455 0.52 -0.5 3,017,085 + BWI76_RS14455 0.85 -0.2 3,017,087 - BWI76_RS14455 0.86 -0.4 3,017,187 + -0.5 3,017,245 - +0.3 3,017,972 - BWI76_RS14460 0.65 -2.0 3,018,128 + BWI76_RS14460 0.83 +0.7 3,018,128 + BWI76_RS14460 0.83 -1.2 3,018,223 + -2.8 3,018,286 - -0.5 3,018,324 - -0.9 3,018,375 - -0.8 3,018,387 - -0.2 3,018,395 - +0.0 3,018,422 - -1.0 3,018,614 + -0.9 3,018,617 - +4.4 3,018,617 - +0.5 3,018,643 + -0.7 3,018,671 + BWI76_RS14465 0.16 +0.2 3,018,671 + BWI76_RS14465 0.16 -0.6 3,018,726 + BWI76_RS14465 0.29 -1.4 3,018,759 + BWI76_RS14465 0.36 -1.6 3,018,967 + BWI76_RS14465 0.83 -0.3 3,018,968 + BWI76_RS14465 0.83 -0.7 3,018,970 + BWI76_RS14465 0.84 +1.6 3,018,983 + BWI76_RS14465 0.86 -0.6 3,018,983 + BWI76_RS14465 0.86 -0.5 3,018,983 + BWI76_RS14465 0.86 -0.2 3,018,991 - BWI76_RS14465 0.88 +0.3 3,019,003 - +0.9 3,019,004 - +1.5 3,019,050 + -0.1 3,019,218 - -0.0
Or see this region's nucleotide sequence