Experiment: KLEB15 MOI 1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13180 and BWI76_RS13185 are separated by 19 nucleotides BWI76_RS13185 and BWI76_RS13190 overlap by 1 nucleotides BWI76_RS13190 and BWI76_RS13195 are separated by 177 nucleotides
BWI76_RS13180: BWI76_RS13180 - multidrug transporter EmrA, at 2,750,382 to 2,751,467
_RS13180
BWI76_RS13185: BWI76_RS13185 - MFS transporter, at 2,751,487 to 2,753,139
_RS13185
BWI76_RS13190: BWI76_RS13190 - MarR family transcriptional regulator, at 2,753,139 to 2,753,570
_RS13190
BWI76_RS13195: BWI76_RS13195 - glutamate dehydrogenase, at 2,753,748 to 2,755,022
_RS13195
Position (kb)
2751
2752
2753
2754 Strain fitness (log2 ratio)
-1
0
1 at 2750.911 kb on + strand, within BWI76_RS13180 at 2750.945 kb on - strand, within BWI76_RS13180 at 2751.173 kb on + strand, within BWI76_RS13180 at 2751.181 kb on - strand, within BWI76_RS13180 at 2751.449 kb on - strand at 2751.889 kb on - strand, within BWI76_RS13185 at 2752.351 kb on - strand, within BWI76_RS13185 at 2752.508 kb on - strand, within BWI76_RS13185 at 2753.635 kb on - strand at 2753.659 kb on - strand at 2753.974 kb on + strand, within BWI76_RS13195
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 1 remove 2,750,911 + BWI76_RS13180 0.49 +1.0 2,750,945 - BWI76_RS13180 0.52 -1.2 2,751,173 + BWI76_RS13180 0.73 +0.0 2,751,181 - BWI76_RS13180 0.74 +1.7 2,751,449 - -1.7 2,751,889 - BWI76_RS13185 0.24 -0.4 2,752,351 - BWI76_RS13185 0.52 -1.6 2,752,508 - BWI76_RS13185 0.62 -0.5 2,753,635 - -0.6 2,753,659 - -0.5 2,753,974 + BWI76_RS13195 0.18 -1.3
Or see this region's nucleotide sequence