Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS23940 and BWI76_RS23945 are separated by 10 nucleotides BWI76_RS23945 and BWI76_RS23950 are separated by 24 nucleotides
BWI76_RS23935: BWI76_RS23935 - methylmalonyl-CoA mutase, at 4,994,947 to 4,997,091
_RS23935
BWI76_RS23940: BWI76_RS23940 - ATPase/protein kinase, at 4,997,084 to 4,998,079
_RS23940
BWI76_RS23945: BWI76_RS23945 - methylmalonyl-CoA decarboxylase, at 4,998,090 to 4,998,875
_RS23945
BWI76_RS23950: BWI76_RS23950 - succinate CoA transferase, at 4,998,900 to 5,000,366
_RS23950
Position (kb)
4998
4999 Strain fitness (log2 ratio)
-2
-1
0
1 at 4997.148 kb on - strand at 4997.426 kb on + strand, within BWI76_RS23940 at 4997.519 kb on + strand, within BWI76_RS23940 at 4997.519 kb on + strand, within BWI76_RS23940 at 4997.701 kb on - strand, within BWI76_RS23940 at 4997.701 kb on - strand, within BWI76_RS23940 at 4997.947 kb on + strand, within BWI76_RS23940 at 4998.334 kb on - strand, within BWI76_RS23945 at 4998.407 kb on + strand, within BWI76_RS23945 at 4998.806 kb on - strand at 4998.891 kb on - strand at 4998.891 kb on - strand at 4998.891 kb on - strand at 4999.206 kb on - strand, within BWI76_RS23950 at 4999.466 kb on + strand, within BWI76_RS23950 at 4999.603 kb on + strand, within BWI76_RS23950 at 4999.746 kb on - strand, within BWI76_RS23950 at 4999.746 kb on - strand, within BWI76_RS23950 at 4999.850 kb on - strand, within BWI76_RS23950 at 4999.873 kb on + strand, within BWI76_RS23950
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 4,997,148 - +0.4 4,997,426 + BWI76_RS23940 0.34 -0.1 4,997,519 + BWI76_RS23940 0.44 +0.4 4,997,519 + BWI76_RS23940 0.44 -0.1 4,997,701 - BWI76_RS23940 0.62 -0.1 4,997,701 - BWI76_RS23940 0.62 +0.1 4,997,947 + BWI76_RS23940 0.87 +0.5 4,998,334 - BWI76_RS23945 0.31 -0.9 4,998,407 + BWI76_RS23945 0.40 -0.7 4,998,806 - +0.7 4,998,891 - -2.1 4,998,891 - +0.9 4,998,891 - -0.0 4,999,206 - BWI76_RS23950 0.21 -0.5 4,999,466 + BWI76_RS23950 0.39 -1.3 4,999,603 + BWI76_RS23950 0.48 +0.0 4,999,746 - BWI76_RS23950 0.58 -1.5 4,999,746 - BWI76_RS23950 0.58 -0.7 4,999,850 - BWI76_RS23950 0.65 -0.3 4,999,873 + BWI76_RS23950 0.66 -0.8
Or see this region's nucleotide sequence