Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21390 and BWI76_RS21395 are separated by 69 nucleotides BWI76_RS21395 and BWI76_RS21400 are separated by 35 nucleotides BWI76_RS21400 and BWI76_RS21405 are separated by 93 nucleotides
BWI76_RS21390: BWI76_RS21390 - thiol disulfide reductase thioredoxin, at 4,477,662 to 4,478,087
_RS21390
BWI76_RS21395: BWI76_RS21395 - DTW domain-containing protein, at 4,478,157 to 4,478,855
_RS21395
BWI76_RS21400: BWI76_RS21400 - protein lysine acetyltransferase, at 4,478,891 to 4,481,569
_RS21400
BWI76_RS21405: BWI76_RS21405 - phosphatidylserine synthase, at 4,481,663 to 4,483,018
_RS21405
Position (kb) 4478
4479
4480
4481
4482 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4
5
6 at 4477.899 kb on + strand, within BWI76_RS21390 at 4477.936 kb on - strand, within BWI76_RS21390 at 4478.299 kb on - strand, within BWI76_RS21395 at 4478.651 kb on + strand, within BWI76_RS21395 at 4478.789 kb on + strand at 4478.813 kb on + strand at 4478.874 kb on + strand at 4479.064 kb on + strand at 4479.262 kb on + strand, within BWI76_RS21400 at 4479.267 kb on + strand, within BWI76_RS21400 at 4479.275 kb on - strand, within BWI76_RS21400 at 4479.437 kb on + strand, within BWI76_RS21400 at 4479.445 kb on - strand, within BWI76_RS21400 at 4479.520 kb on + strand, within BWI76_RS21400 at 4479.694 kb on + strand, within BWI76_RS21400 at 4479.859 kb on - strand, within BWI76_RS21400 at 4480.091 kb on - strand, within BWI76_RS21400 at 4480.295 kb on - strand, within BWI76_RS21400 at 4480.340 kb on + strand, within BWI76_RS21400 at 4480.845 kb on + strand, within BWI76_RS21400 at 4480.930 kb on - strand, within BWI76_RS21400 at 4481.019 kb on + strand, within BWI76_RS21400 at 4481.022 kb on - strand, within BWI76_RS21400 at 4481.086 kb on + strand, within BWI76_RS21400 at 4481.112 kb on - strand, within BWI76_RS21400 at 4481.470 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 4,477,899 + BWI76_RS21390 0.56 +0.2 4,477,936 - BWI76_RS21390 0.64 -0.2 4,478,299 - BWI76_RS21395 0.20 +0.3 4,478,651 + BWI76_RS21395 0.71 +0.4 4,478,789 + +0.6 4,478,813 + +0.4 4,478,874 + -2.8 4,479,064 + -0.3 4,479,262 + BWI76_RS21400 0.14 +0.7 4,479,267 + BWI76_RS21400 0.14 +0.1 4,479,275 - BWI76_RS21400 0.14 +0.7 4,479,437 + BWI76_RS21400 0.20 +0.4 4,479,445 - BWI76_RS21400 0.21 -3.8 4,479,520 + BWI76_RS21400 0.23 +0.2 4,479,694 + BWI76_RS21400 0.30 -0.6 4,479,859 - BWI76_RS21400 0.36 -0.5 4,480,091 - BWI76_RS21400 0.45 +1.1 4,480,295 - BWI76_RS21400 0.52 +0.6 4,480,340 + BWI76_RS21400 0.54 +0.6 4,480,845 + BWI76_RS21400 0.73 +0.4 4,480,930 - BWI76_RS21400 0.76 +0.7 4,481,019 + BWI76_RS21400 0.79 +0.8 4,481,022 - BWI76_RS21400 0.80 +0.8 4,481,086 + BWI76_RS21400 0.82 +1.2 4,481,112 - BWI76_RS21400 0.83 +5.9 4,481,470 + -1.4
Or see this region's nucleotide sequence