Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS21225 and BWI76_RS21230 overlap by 14 nucleotides BWI76_RS21230 and BWI76_RS21235 overlap by 15 nucleotides BWI76_RS21235 and BWI76_RS21240 overlap by 56 nucleotides
BWI76_RS21225: BWI76_RS21225 - serine hydroxymethyltransferase, at 4,445,622 to 4,446,875
_RS21225
BWI76_RS21230: BWI76_RS21230 - hypothetical protein, at 4,446,862 to 4,447,091
_RS21230
BWI76_RS21235: BWI76_RS21235 - hypothetical protein, at 4,447,077 to 4,447,256
_RS21235
BWI76_RS21240: BWI76_RS21240 - flavohemoprotein, at 4,447,201 to 4,448,391
_RS21240
Position (kb) 4447
4448 Strain fitness (log2 ratio) -1
0
1
2 at 4446.973 kb on - strand, within BWI76_RS21230 at 4447.198 kb on + strand, within BWI76_RS21235 at 4447.259 kb on - strand at 4447.357 kb on - strand, within BWI76_RS21240 at 4447.482 kb on + strand, within BWI76_RS21240 at 4447.482 kb on + strand, within BWI76_RS21240 at 4447.584 kb on - strand, within BWI76_RS21240 at 4447.854 kb on - strand, within BWI76_RS21240 at 4448.039 kb on - strand, within BWI76_RS21240 at 4448.094 kb on - strand, within BWI76_RS21240 at 4448.109 kb on - strand, within BWI76_RS21240 at 4448.201 kb on - strand, within BWI76_RS21240
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 4,446,973 - BWI76_RS21230 0.48 +1.0 4,447,198 + BWI76_RS21235 0.67 -1.3 4,447,259 - +2.1 4,447,357 - BWI76_RS21240 0.13 +0.1 4,447,482 + BWI76_RS21240 0.24 +0.3 4,447,482 + BWI76_RS21240 0.24 -0.3 4,447,584 - BWI76_RS21240 0.32 +0.5 4,447,854 - BWI76_RS21240 0.55 +0.3 4,448,039 - BWI76_RS21240 0.70 +0.5 4,448,094 - BWI76_RS21240 0.75 +0.9 4,448,109 - BWI76_RS21240 0.76 +0.3 4,448,201 - BWI76_RS21240 0.84 -0.4
Or see this region's nucleotide sequence