Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS13185 and BWI76_RS13190 overlap by 1 nucleotides BWI76_RS13190 and BWI76_RS13195 are separated by 177 nucleotides BWI76_RS13195 and BWI76_RS13200 overlap by 26 nucleotides BWI76_RS13200 and BWI76_RS13205 are separated by 202 nucleotides
BWI76_RS13185: BWI76_RS13185 - MFS transporter, at 2,751,487 to 2,753,139
_RS13185
BWI76_RS13190: BWI76_RS13190 - MarR family transcriptional regulator, at 2,753,139 to 2,753,570
_RS13190
BWI76_RS13195: BWI76_RS13195 - glutamate dehydrogenase, at 2,753,748 to 2,755,022
_RS13195
BWI76_RS13200: BWI76_RS13200 - hypothetical protein, at 2,754,997 to 2,755,230
_RS13200
BWI76_RS13205: BWI76_RS13205 - hypothetical protein, at 2,755,433 to 2,756,224
_RS13205
Position (kb)
2753
2754
2755
2756 Strain fitness (log2 ratio)
-2
-1
0
1 at 2753.635 kb on - strand at 2753.659 kb on - strand at 2753.974 kb on + strand, within BWI76_RS13195 at 2754.215 kb on - strand, within BWI76_RS13195 at 2754.261 kb on - strand, within BWI76_RS13195 at 2755.074 kb on + strand, within BWI76_RS13200 at 2755.074 kb on + strand, within BWI76_RS13200 at 2755.074 kb on + strand, within BWI76_RS13200 at 2755.074 kb on + strand, within BWI76_RS13200 at 2755.074 kb on + strand, within BWI76_RS13200 at 2755.074 kb on + strand, within BWI76_RS13200 at 2755.074 kb on + strand, within BWI76_RS13200 at 2755.075 kb on + strand, within BWI76_RS13200 at 2755.082 kb on - strand, within BWI76_RS13200 at 2755.082 kb on - strand, within BWI76_RS13200 at 2755.407 kb on + strand at 2755.662 kb on + strand, within BWI76_RS13205 at 2755.757 kb on + strand, within BWI76_RS13205 at 2755.899 kb on - strand, within BWI76_RS13205 at 2755.915 kb on - strand, within BWI76_RS13205 at 2755.951 kb on + strand, within BWI76_RS13205
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 2,753,635 - -0.0 2,753,659 - +0.6 2,753,974 + BWI76_RS13195 0.18 -0.8 2,754,215 - BWI76_RS13195 0.37 -1.1 2,754,261 - BWI76_RS13195 0.40 -0.8 2,755,074 + BWI76_RS13200 0.33 -1.6 2,755,074 + BWI76_RS13200 0.33 -0.7 2,755,074 + BWI76_RS13200 0.33 +0.8 2,755,074 + BWI76_RS13200 0.33 -0.7 2,755,074 + BWI76_RS13200 0.33 -1.0 2,755,074 + BWI76_RS13200 0.33 -0.1 2,755,074 + BWI76_RS13200 0.33 -1.4 2,755,075 + BWI76_RS13200 0.33 +0.7 2,755,082 - BWI76_RS13200 0.36 -1.6 2,755,082 - BWI76_RS13200 0.36 -2.1 2,755,407 + -0.6 2,755,662 + BWI76_RS13205 0.29 -0.8 2,755,757 + BWI76_RS13205 0.41 +1.0 2,755,899 - BWI76_RS13205 0.59 +0.3 2,755,915 - BWI76_RS13205 0.61 +1.2 2,755,951 + BWI76_RS13205 0.65 -2.1
Or see this region's nucleotide sequence