Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10365 and BWI76_RS10370 are separated by 15 nucleotides BWI76_RS10370 and BWI76_RS10375 are separated by 16 nucleotides BWI76_RS10375 and BWI76_RS10380 are separated by 13 nucleotides BWI76_RS10380 and BWI76_RS10385 are separated by 142 nucleotides
BWI76_RS10365: BWI76_RS10365 - class IV aminotransferase, at 2,195,080 to 2,196,045
_RS10365
BWI76_RS10370: BWI76_RS10370 - ABC transporter-like protein, at 2,196,061 to 2,196,840
_RS10370
BWI76_RS10375: BWI76_RS10375 - polar amino acid ABC transporter inner membrane subunit, at 2,196,857 to 2,197,522
_RS10375
BWI76_RS10380: BWI76_RS10380 - polar amino acid ABC transporter inner membrane subunit, at 2,197,536 to 2,198,207
_RS10380
BWI76_RS10385: BWI76_RS10385 - MmgE/PrpD family protein, at 2,198,350 to 2,199,759
_RS10385
Position (kb)
2196
2197
2198 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2196.102 kb on - strand at 2196.210 kb on + strand, within BWI76_RS10370 at 2196.293 kb on - strand, within BWI76_RS10370 at 2196.369 kb on + strand, within BWI76_RS10370 at 2196.452 kb on - strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.630 kb on + strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.638 kb on - strand, within BWI76_RS10370 at 2196.844 kb on - strand at 2196.909 kb on + strand at 2196.924 kb on + strand, within BWI76_RS10375 at 2197.144 kb on - strand, within BWI76_RS10375 at 2197.233 kb on - strand, within BWI76_RS10375 at 2197.334 kb on - strand, within BWI76_RS10375 at 2197.551 kb on - strand at 2197.617 kb on - strand, within BWI76_RS10380 at 2197.683 kb on - strand, within BWI76_RS10380 at 2198.137 kb on + strand, within BWI76_RS10380 at 2198.140 kb on + strand at 2198.209 kb on + strand at 2198.262 kb on + strand at 2198.433 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 2,196,102 - +0.2 2,196,210 + BWI76_RS10370 0.19 +0.3 2,196,293 - BWI76_RS10370 0.30 -0.8 2,196,369 + BWI76_RS10370 0.39 -0.3 2,196,452 - BWI76_RS10370 0.50 -0.2 2,196,630 + BWI76_RS10370 0.73 +0.3 2,196,630 + BWI76_RS10370 0.73 +0.4 2,196,630 + BWI76_RS10370 0.73 +0.3 2,196,638 - BWI76_RS10370 0.74 +2.9 2,196,638 - BWI76_RS10370 0.74 +0.1 2,196,638 - BWI76_RS10370 0.74 +0.2 2,196,844 - +0.1 2,196,909 + -3.1 2,196,924 + BWI76_RS10375 0.10 -1.2 2,197,144 - BWI76_RS10375 0.43 -0.1 2,197,233 - BWI76_RS10375 0.56 -1.2 2,197,334 - BWI76_RS10375 0.72 -0.1 2,197,551 - +0.2 2,197,617 - BWI76_RS10380 0.12 +0.7 2,197,683 - BWI76_RS10380 0.22 -0.7 2,198,137 + BWI76_RS10380 0.89 -0.4 2,198,140 + -0.6 2,198,209 + -0.4 2,198,262 + -0.0 2,198,433 + +1.6
Or see this region's nucleotide sequence