Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS10045 and BWI76_RS10050 are separated by 190 nucleotides BWI76_RS10050 and BWI76_RS10055 are separated by 15 nucleotides BWI76_RS10055 and BWI76_RS10060 are separated by 51 nucleotides
BWI76_RS10045: BWI76_RS10045 - 23S rRNA (cytosine(1962)-C(5))-methyltransferase, at 2,128,323 to 2,129,525
_RS10045
BWI76_RS10050: BWI76_RS10050 - outer membrane protein, at 2,129,716 to 2,130,270
_RS10050
BWI76_RS10055: BWI76_RS10055 - AraC family transcriptional regulator, at 2,130,286 to 2,131,074
_RS10055
BWI76_RS10060: BWI76_RS10060 - acylphosphatase, at 2,131,126 to 2,131,407
_RS10060
Position (kb)
2129
2130
2131 Strain fitness (log2 ratio)
-1
0
1 at 2128.762 kb on - strand, within BWI76_RS10045 at 2128.900 kb on - strand, within BWI76_RS10045 at 2129.803 kb on + strand, within BWI76_RS10050 at 2129.811 kb on - strand, within BWI76_RS10050 at 2129.980 kb on + strand, within BWI76_RS10050 at 2130.069 kb on - strand, within BWI76_RS10050 at 2130.140 kb on + strand, within BWI76_RS10050 at 2130.164 kb on + strand, within BWI76_RS10050 at 2130.246 kb on + strand at 2130.784 kb on + strand, within BWI76_RS10055 at 2130.866 kb on + strand, within BWI76_RS10055 at 2131.088 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 2,128,762 - BWI76_RS10045 0.36 -1.1 2,128,900 - BWI76_RS10045 0.48 -0.1 2,129,803 + BWI76_RS10050 0.16 +0.8 2,129,811 - BWI76_RS10050 0.17 -0.3 2,129,980 + BWI76_RS10050 0.48 -0.1 2,130,069 - BWI76_RS10050 0.64 -0.0 2,130,140 + BWI76_RS10050 0.76 +0.1 2,130,164 + BWI76_RS10050 0.81 -1.1 2,130,246 + -0.4 2,130,784 + BWI76_RS10055 0.63 -0.2 2,130,866 + BWI76_RS10055 0.74 +0.0 2,131,088 + -0.2
Or see this region's nucleotide sequence