Experiment: KLEB15 MOI 10
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt BWI76_RS27595 and BWI76_RS27600 are separated by 291 nucleotides BWI76_RS27600 and BWI76_RS27605 are separated by 38 nucleotides BWI76_RS27605 and BWI76_RS27610 are separated by 326 nucleotides BWI76_RS27610 and BWI76_RS27615 are separated by 95 nucleotides
BWI76_RS27595: BWI76_RS27595 - glycoside-pentoside-hexuronide family transporter, at 5,728,796 to 5,730,190
_RS27595
BWI76_RS27600: BWI76_RS27600 - tRNA-Sec, at 5,730,482 to 5,730,576
_RS27600
BWI76_RS27605: BWI76_RS27605 - hypothetical protein, at 5,730,615 to 5,730,806
_RS27605
BWI76_RS27610: BWI76_RS27610 - hydroxyacid dehydrogenase, at 5,731,133 to 5,731,647
_RS27610
BWI76_RS27615: BWI76_RS27615 - putative Shikimate kinase, at 5,731,743 to 5,732,348
_RS27615
Position (kb)
5730
5731 Strain fitness (log2 ratio)
-1
0
1
2 at 5729.699 kb on - strand, within BWI76_RS27595 at 5729.754 kb on + strand, within BWI76_RS27595 at 5729.764 kb on + strand, within BWI76_RS27595 at 5729.767 kb on - strand, within BWI76_RS27595 at 5729.792 kb on - strand, within BWI76_RS27595 at 5729.820 kb on + strand, within BWI76_RS27595 at 5729.820 kb on + strand, within BWI76_RS27595 at 5729.820 kb on + strand, within BWI76_RS27595 at 5729.820 kb on + strand, within BWI76_RS27595 at 5729.828 kb on - strand, within BWI76_RS27595 at 5729.847 kb on - strand at 5729.865 kb on + strand, within BWI76_RS27595 at 5729.895 kb on + strand, within BWI76_RS27595 at 5729.903 kb on - strand, within BWI76_RS27595 at 5730.549 kb on - strand, within BWI76_RS27600 at 5730.559 kb on - strand, within BWI76_RS27600 at 5730.559 kb on - strand, within BWI76_RS27600 at 5730.602 kb on - strand at 5730.673 kb on - strand, within BWI76_RS27605 at 5730.689 kb on + strand, within BWI76_RS27605 at 5730.856 kb on + strand at 5730.866 kb on + strand at 5730.987 kb on - strand at 5731.123 kb on - strand at 5731.161 kb on + strand at 5731.265 kb on + strand, within BWI76_RS27610 at 5731.273 kb on - strand, within BWI76_RS27610 at 5731.298 kb on + strand, within BWI76_RS27610 at 5731.576 kb on - strand, within BWI76_RS27610 at 5731.627 kb on - strand at 5731.627 kb on - strand at 5731.650 kb on - strand at 5731.710 kb on + strand at 5731.718 kb on - strand at 5731.718 kb on - strand at 5731.727 kb on - strand at 5731.727 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction KLEB15 MOI 10 remove 5,729,699 - BWI76_RS27595 0.65 -1.7 5,729,754 + BWI76_RS27595 0.69 +0.3 5,729,764 + BWI76_RS27595 0.69 +0.3 5,729,767 - BWI76_RS27595 0.70 -0.8 5,729,792 - BWI76_RS27595 0.71 +0.2 5,729,820 + BWI76_RS27595 0.73 -0.2 5,729,820 + BWI76_RS27595 0.73 +0.4 5,729,820 + BWI76_RS27595 0.73 -0.6 5,729,820 + BWI76_RS27595 0.73 +0.0 5,729,828 - BWI76_RS27595 0.74 +0.7 5,729,847 - +2.1 5,729,865 + BWI76_RS27595 0.77 -0.3 5,729,895 + BWI76_RS27595 0.79 +1.1 5,729,903 - BWI76_RS27595 0.79 +0.1 5,730,549 - BWI76_RS27600 0.71 +0.0 5,730,559 - BWI76_RS27600 0.81 +0.2 5,730,559 - BWI76_RS27600 0.81 -0.6 5,730,602 - +0.4 5,730,673 - BWI76_RS27605 0.30 -1.7 5,730,689 + BWI76_RS27605 0.39 -0.9 5,730,856 + -1.0 5,730,866 + +0.3 5,730,987 - -0.3 5,731,123 - -1.0 5,731,161 + -0.1 5,731,265 + BWI76_RS27610 0.26 +0.3 5,731,273 - BWI76_RS27610 0.27 +0.3 5,731,298 + BWI76_RS27610 0.32 +0.3 5,731,576 - BWI76_RS27610 0.86 +0.0 5,731,627 - +0.4 5,731,627 - -0.6 5,731,650 - -0.9 5,731,710 + +0.7 5,731,718 - -0.9 5,731,718 - +0.3 5,731,727 - +0.0 5,731,727 - +0.8
Or see this region's nucleotide sequence